Diabetic Retinopathy & Medical Retina - aioseducation

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Diabetic Retinopathy and Medical Retina Free Papers Group I: Healthy volunteers (control) Group II: Patients with type 2 Diabetes Mellitus Group III: Diabetic retinopathy- non proliferative (NPDR) Group IV: Proliferative Diabetic Retinopathy (PDR) Group V : Macular Hole (MH) (Disease control) • Consents were obtained for vitreous and blood specimen separately • Clinical proforma was filled and kept in the Department of Biochemistry and Cell Biology. • Blood/urine samples were collected from all these groups • Routine biochemical parameters were done and if they fell into the criteria, then the samples were used for the rest of the analysis. • Based on the clinical proforma and lab investigations patients were grouped. Sample collection details The fasting blood was collected from the patient by using BD-vacutainer system. 2 ml- Fluoride tube : Plasma for fasting glucose estimation Whole blood (EDTA tube) : HBA1C 4 ml- Plain serum tube : lipid profile, /cytokines/growth factors Urine : microalbumin Undiluted Vitreous : 250-500 µL • The fasting blood samples and urine samples were collected before the surgery and then aliquoted and stored in -80°C. • The vitreous sample was centrifuged, (using a cooling centrifuge) aliquoted and then stored in -80°C. RESULTS Table 1 shows age, anthropometric measurements and the levels of biochemical parameters such as total cholesterol,triglycerides, and µ-albumin. Among all the parameters examined, HBA1C, triglycerides, and Urine µ-albumin showed a directly proportional relation to diabetic progression. Tables 2 shows the vitreous PEDF levels in PDR and MH. The vitreous PEDF levels were increased in PDR group when compared to the macular hole. Table 3 shows the vitreous VEGF levels in PDR and macular hole. VEGF levels were increased in PDR group when compared to macular hole. 881
70th AIOC Proceedings, Cochin 2012 Table 1: Anthropometric measurements and biochemical parameters in control and diabetic groups 882 Group-I Group-II Group-III Group-IV (Control) (Diabetes) (NPDR) (PDR) (n=6) (n=8) (n=4) (n=10) Age (in years) 43.26 ± 6.93 58.36 ± 13.51 48.50 ± 5.51 48.727 ± 6.326 BMI (m2/kg) 25.59± 3.07 26.27 ± 4.26 24.29 ± 3.41 24.02 ± 3.78 Fasting Glucose 94.0 ± 7.90 143 ± 28.07* 153.00 ± 84.31 158.364 ± 79.873 (mg/dL) HBA1C (%) 5.37 ± 0.45 6.76 ± 1.69 7.38 ± 1.57* 8.35 ± 2.24* Total cholesterol 182.17 ± 21.78 170.29 ±39.87 136.75 ±15.09 199.100 ± 77.331 (mg/dL) Triglycerides 116.0 ± 35.03 108 ± 37.68 110.75 ± 34.73 281.200 ± 253.244 (mg/dL) TC/HDL-C 3.87±0.72 3.63±0.91 3.38 ± 0.97 4.917 ± 1.788 μ-Albumin 8.95±6.07 49.56±70.77 110.73 ± 105.01 135.67± 97.98 *P
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