High performance capillary electrophoresis - T.E.A.M.
High performance capillary electrophoresis - T.E.A.M.
High performance capillary electrophoresis - T.E.A.M.
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Instrumentation/Operation<br />
Method Mass detection Concentration Advantages/<br />
limit (moles) detection disadvantages<br />
limit (molar)*<br />
UV-Vis 10 -13 -10 -15 10 -5 -10 -8 • Universal<br />
absorption<br />
• Diode array offers spectral<br />
information<br />
Fluorescence 10 -15 10 -11 10 -7 -10 -9 • Sensitive<br />
• Usually requires sample<br />
derivatization<br />
Laser-induced 10 -18 -10 -20 10 -14 -10 -16 • Extremely sensitive<br />
fluorescence<br />
• Usually requires sample<br />
derivatization<br />
• Expensive<br />
Amperometry 10 -l8 -10 -19 10 -10 -10 -11 • Sensitive<br />
• Selective but useful<br />
only for electroactive<br />
analyses<br />
• Requires special electronics<br />
and <strong>capillary</strong><br />
modification<br />
Conductivity 10 -15 -10 -16 10 -7 -10 -8 • Universal<br />
• Requires special<br />
electronics and <strong>capillary</strong><br />
modification<br />
Mass 10 -16 -10 -17 10 -8 -10 -9 • Sensitive and offers<br />
spectrometry<br />
structural information<br />
• Interface between CE<br />
and MS complicated<br />
Indirect UV, 10-100 – •Universal<br />
fluorescence, times less • lower sensitivity than<br />
amperometry than direct direct methods<br />
method<br />
Others:<br />
Radioactivity, thermal lens, refractive index, circular dichroism, Raman<br />
Table 19<br />
*assume10 nl injection volume<br />
98
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