High performance capillary electrophoresis - T.E.A.M.
High performance capillary electrophoresis - T.E.A.M.
High performance capillary electrophoresis - T.E.A.M.
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Instrumentation/Operation<br />
Pressure<br />
Sample<br />
Sample<br />
Hydrodynamic<br />
Vacuum<br />
a)<br />
b)<br />
c)<br />
Siphoning<br />
Sample<br />
Electrokinetic<br />
d)<br />
Sample<br />
Figure 51<br />
Methods of sample injection<br />
The volume of sample loaded will be a function of the<br />
<strong>capillary</strong> dimensions, the viscosity of the buffer in the<br />
<strong>capillary</strong>, the applied pressure, and the time. This volume<br />
can be using the Hagen-Poiseuille equation.<br />
DPd<br />
Volume = 4 p t<br />
(28)<br />
128 hL<br />
84
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