High performance capillary electrophoresis - T.E.A.M.

Modes
EOF. Since the EOF is generally faster than the migration
velocity of the micelles at neutral or basic pH, the net
movement is in the direction of the EOF. During migration,
the micelles can interact with solutes in a chromatographic
manner through both hydrophobic and electrostatic interactions.
For neutral species, it is only partitioning in and out of the
micelle that effects the separation. The more the solute
interacts with the micelle the longer is its migration time
since the micelle carries it against the EOF. When the solute
is not in contact with the micelle it is simply carried with
the EOF. The more hydrophobic compounds interact more
strongly with the micelle and are “retained” longer. The
overall MEKC separation process is depicted schematically
in figure 35.
Figure 35
Separation in MEKC
s = solute
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