High performance capillary electrophoresis - T.E.A.M.

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A primer High performance capillary electrophoresis An introduction David Heiger 3
Page 1: An introduction High performance ca
Page 5 and 6: Acknowledgement The author sincerel
Page 7 and 8: CE is characterized by imaginative
Page 9 and 10: 3.4 Capillary isoelectric focusing
Page 11 and 12: Chapter 1 Introduction 11
Page 13 and 14: separation, detection, quantitative
Page 15 and 16: Chapter 2 Principles of capillary e
Page 17 and 18: 75-µm id fused silica capillaries.
Page 19 and 20: 0 µ Full charge Zero charge α = 0
Page 21 and 22: a) Principles b) c) Figure 5 Develo
Page 23 and 24: e disrupted, however, if the capill
Page 25 and 26: Variable Result Comment Principles
Page 27 and 28: time and other experimental paramet
Page 29 and 30: Under ideal conditions (that is, sm
Page 31 and 32: Source Longitudinal diffusion Joule
Page 33 and 34: Temperature Principles Capillary wa
Page 35 and 36: ent) with radius. Capillaries with
Page 37 and 38: occur. The primary causes of adsorp
Page 39 and 40: Figure 17 Influence of buffer conce
Page 41 and 42: These peak shape distortions are ca
Page 43 and 44: The resolution of two components ca
Page 45 and 46: Chapter 3 Modes of operation 45
Page 47 and 48: Modes 3.1 Capillary zone electropho
Page 49 and 50: Modes Additive Example Use Surfacta
Page 51 and 52: Modes pH 2.6 pH 8.2 32 115 98 104 t
Page 53 and 54:
Modes Absorbance 0 10 20 30 40 Rete
Page 55 and 56:
Modes 1 2 3 45 6 16 20 24 28 32 36
Page 57 and 58:
Modes EOF [mm/min] 50 40 30 20 10 T
Page 59 and 60:
Modes a) CH 3COONa-CH 3COOH difficu
Page 61 and 62:
Modes CA a) liquid chromatography,
Page 63 and 64:
Modes EOF. Since the EOF is general
Page 65 and 66:
Modes From equation (25), resolutio
Page 67 and 68:
Modes micelle migration varies and
Page 69 and 70:
Modes The versatility of MEKC is fu
Page 71 and 72:
Modes smaller mesh spacings (or por
Page 73 and 74:
Modes CGE can be altered by the add
Page 75 and 76:
Modes 3.4 Capillary isoelectric foc
Page 77 and 78:
Modes 3.4.1 Applications of CIEF CI
Page 79 and 80:
Modes UV 254 nm 0.001 AU 1 2 3 ITP
Page 81 and 82:
Chapter 4 Instrumental and operatio
Page 83 and 84:
Start Start a) Stop b) Stop c) volu
Page 85 and 86:
where DP = pressure difference acro
Page 87 and 88:
4.1.2 Electrokinetic injection Elec
Page 89 and 90:
The simplest way to perform a stack
Page 91 and 92:
as optic cells and GC columns. Simi
Page 93 and 94:
Batch-to-batch reproducibility is h
Page 95 and 96:
4.2.4 Migration time/mobility repro
Page 97 and 98:
pre-concentration methods are often
Page 99 and 100:
detectors the absorbance of a solut
Page 101 and 102:
Detector response [arbitrary units]
Page 103 and 104:
Peak height is generally not as use
Page 105 and 106:
Figure 62 Photograph of a dye front
Page 107 and 108:
Figure 64 Electropherogram and corr
Page 109 and 110:
4.3.7.4 Confirming peak identity Mi
Page 111 and 112:
a) 0.005 AU 19 23 p(dA) 20 0.001 AU
Page 113 and 114:
Chapter 5 List of abbreviations 113
Page 115 and 116:
Abbreviation Full Name Unit (SI) Un
Page 117 and 118:
Abbreviation Full Name Unit (SI) Un
Page 119 and 120:
Chapter 6 References and bibliograp
Page 121 and 122:
8 M.T. Ackermans, F.M. Everaerts, J
Page 123 and 124:
22 D.N. Heiger, A.S. Cohen, B.L. Ka
Page 125 and 126:
Guzmann, Norberto A. (Ed)., “Capi
Page 127 and 128:
Chapter 7 Index 127
Page 129 and 130:
Index C Calibration curve, 97, 100,
Page 131 and 132:
Index magnitude, 22, 21, 93 marker,
Page 133 and 134:
Index identity, 109 purity, 108 sha
Page 135 and 136:
Index V Viscosity, 24, 32, 84, 85,
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