High performance capillary electrophoresis - T.E.A.M.

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Introduction sis, methods for on-capillary sample preconcentration to improve sensitivity, and development of capillary coatings to both control electroosmotic flow and limit solute-wall interactions. Undoubtedly, as more researchers use CE and disseminate results, it will become a standard technique in separation science. 1.3 Characteristics of CE • Electrophoresis is performed in narrow-bore (25- to 75-mm id), fused silica capillaries • High voltages (10 to 30 kV) and high electric fields (100 to 500 V/cm) are applied across the capillary • High resistance of the capillary limits current generation and internal heating • High efficiency (N>10 5 to 10 6 ) and short analysis time • Detection performed on-capillary (no external detection cell) • Small sample volume required (1 to 50 nl injected) • Numerous modes to vary selectivity and wide application range • Operates in aqueous media • Simple methods development • Automated instrumentation 14
Chapter 2 Principles of capillary electrophoresis 15
Page 1: An introduction High performance ca
Page 4 and 5: Copyright © 2000 Agilent Technolog
Page 6 and 7: Foreword Capillary electrophoresis
Page 8 and 9: Table of content Foreword .........
Page 10 and 11: Scope The purpose of this book is t
Page 12 and 13: Introduction 1.1 High performance c
Page 16 and 17: Principles 2.1 Historical backgroun
Page 18 and 19: Principles that ion. The mobility i
Page 20 and 21: Principles the exact pI of fused si
Page 22 and 23: Principles µ EOF × 10 -4 (cm 2 /
Page 24 and 25: Principles For the analysis of smal
Page 26 and 27: Principles µ EOF ( × 10 -4 cm 2 /
Page 28 and 29: Principles Total length Effective l
Page 30 and 31: Principles Note that equation (15)
Page 32 and 33: Principles determined by the capill
Page 34 and 35: Principles Current (uA) 300 250 200
Page 36 and 37: Principles The contribution of inje
Page 38 and 39: Principles k' H N H, µm 0.001 0.58
Page 40 and 41: Principles Figure 19 Electrodispers
Page 42 and 43: Principles rapidly eluting ions, th
Page 44 and 45: Principles 44
Page 46 and 47: Modes Mode Capillary zone electroph
Page 48 and 49: Modes 3.1.1 Selectivity and the use
Page 50 and 51: Modes Name pK a Phosphate 2.12 (pK
Page 52 and 53: Modes EOF No flow Figure 22 Elimina
Page 54 and 55: Modes Absorbance 214 nm 0.05 0.04 0
Page 56 and 57: Modes Type Comment Silylation coupl
Page 58 and 59: Modes Type Result Comment Extremes
Page 60 and 61: Modes Figure 29 CZE of reversed pha
Page 62 and 63: Modes Figure 33 Ion analysis of fer
Page 64 and 65:
Modes The separation mechanism of n
Page 66 and 67:
Modes the stationary phase in LC. S
Page 68 and 69:
Modes Amplitude 2 a) with a migrati
Page 70 and 71:
Modes CGE t = 0 t > 0 Polymer matri
Page 72 and 73:
Modes Crosslinked polyacrylamide, a
Page 74 and 75:
Modes a) ds 500 base pairs This sam
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Modes and resolution with respect t
Page 78 and 79:
Modes 3.5 Capillary isotachophoresi
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Modes 80
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Instrumentation/Operation Diode-arr
Page 84 and 85:
Instrumentation/Operation Pressure
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Instrumentation/Operation If sensit
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Instrumentation/Operation Despite q
Page 90 and 91:
Instrumentation/Operation T, ˚C 10
Page 92 and 93:
Instrumentation/Operation 4.2.1.1 C
Page 94 and 95:
Instrumentation/Operation However,
Page 96 and 97:
Instrumentation/Operation Calculati
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Instrumentation/Operation Method Ma
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Instrumentation/Operation 4.3.3 Lin
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Instrumentation/Operation Area (arb
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Instrumentation/Operation 4.3.6 Ext
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Instrumentation/Operation light int
Page 108 and 109:
Instrumentation/Operation 4.3.7.2 Q
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Instrumentation/Operation the capab
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Instrumentation/Operation mAU 140 1
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Abbreviations Abbreviation Full Nam
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
References/bibliography References
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References/bibliography 15 W.C. Bru
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References/bibliography 29 R.L. Chi
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References/bibliography 126
Page 128 and 129:
Index A Absorption. See Detection,
Page 130 and 131:
Index D DAD. See Detection, diode-a
Page 132 and 133:
Index I Ionic strength , 22, 25, 26
Page 134 and 135:
Index selectivity, 47 Response time
Page 136:
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