High performance capillary electrophoresis - T.E.A.M.

Introduction
sis, methods for on-capillary sample preconcentration to
improve sensitivity, and development of capillary coatings
to both control electroosmotic flow and limit solute-wall
interactions. Undoubtedly, as more researchers use CE and
disseminate results, it will become a standard technique in
separation science.
1.3 Characteristics
of CE
• Electrophoresis is performed in narrow-bore
(25- to 75-mm id), fused silica capillaries
• High voltages (10 to 30 kV) and high electric fields
(100 to 500 V/cm) are applied across the capillary
• High resistance of the capillary limits current generation
and internal heating
• High efficiency (N>10 5 to 10 6 ) and short analysis time
• Detection performed on-capillary
(no external detection cell)
• Small sample volume required (1 to 50 nl injected)
• Numerous modes to vary selectivity and wide
application range
• Operates in aqueous media
• Simple methods development
• Automated instrumentation
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