High performance capillary electrophoresis - T.E.A.M.

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www.agilent.com/chem Copyright © 2000 Agilent Technologies The information in this publication is subject to change without notice. All Rights Reserved. Reproduction, adaptation or translation without prior written permission is prohibited, except as allowed under the copyright laws. Printed in Germany 03/00 Publication Number 5968-9963E 136
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An introduction High performance ca
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Copyright © 2000 Agilent Technolog
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Foreword Capillary electrophoresis
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Table of content Foreword .........
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Scope The purpose of this book is t
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Introduction 1.1 High performance c
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Introduction sis, methods for on-ca
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Principles 2.1 Historical backgroun
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Principles that ion. The mobility i
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Principles the exact pI of fused si
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Principles µ EOF × 10 -4 (cm 2 /
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Principles For the analysis of smal
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Principles µ EOF ( × 10 -4 cm 2 /
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Principles Total length Effective l
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Principles Note that equation (15)
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Principles determined by the capill
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Principles Current (uA) 300 250 200
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Principles The contribution of inje
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Principles k' H N H, µm 0.001 0.58
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Principles Figure 19 Electrodispers
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Principles rapidly eluting ions, th
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Principles 44
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Modes Mode Capillary zone electroph
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Modes 3.1.1 Selectivity and the use
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Modes Name pK a Phosphate 2.12 (pK
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Modes EOF No flow Figure 22 Elimina
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Modes Absorbance 214 nm 0.05 0.04 0
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Modes Type Comment Silylation coupl
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Modes Type Result Comment Extremes
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Modes Figure 29 CZE of reversed pha
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Modes Figure 33 Ion analysis of fer
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Modes The separation mechanism of n
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Modes the stationary phase in LC. S
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Modes Amplitude 2 a) with a migrati
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Modes CGE t = 0 t > 0 Polymer matri
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Modes Crosslinked polyacrylamide, a
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Modes a) ds 500 base pairs This sam
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Modes and resolution with respect t
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Modes 3.5 Capillary isotachophoresi
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Modes 80
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Instrumentation/Operation Diode-arr
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Instrumentation/Operation Pressure
Page 86 and 87: Instrumentation/Operation If sensit
Page 88 and 89: Instrumentation/Operation Despite q
Page 90 and 91: Instrumentation/Operation T, ˚C 10
Page 92 and 93: Instrumentation/Operation 4.2.1.1 C
Page 94 and 95: Instrumentation/Operation However,
Page 96 and 97: Instrumentation/Operation Calculati
Page 98 and 99: Instrumentation/Operation Method Ma
Page 100 and 101: Instrumentation/Operation 4.3.3 Lin
Page 102 and 103: Instrumentation/Operation Area (arb
Page 104 and 105: Instrumentation/Operation 4.3.6 Ext
Page 106 and 107: Instrumentation/Operation light int
Page 108 and 109: Instrumentation/Operation 4.3.7.2 Q
Page 110 and 111: Instrumentation/Operation the capab
Page 112 and 113: Instrumentation/Operation mAU 140 1
Page 114 and 115: Abbreviations Abbreviation Full Nam
Page 120 and 121: References/bibliography References
Page 122 and 123: References/bibliography 15 W.C. Bru
Page 124 and 125: References/bibliography 29 R.L. Chi
Page 126 and 127: References/bibliography 126
Page 128 and 129: Index A Absorption. See Detection,
Page 130 and 131: Index D DAD. See Detection, diode-a
Page 132 and 133: Index I Ionic strength , 22, 25, 26
Page 134 and 135: Index selectivity, 47 Response time
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High performance capillary electrophoresis - T.E.A.M.

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