High performance capillary electrophoresis - T.E.A.M.

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Index selectivity, 47 Response time. See Detection, response time Restriction fragments, 29, 54, 70, 71-74 S Sample See slso Injection concentration, 40, 78, 79, 87, 96, 97, 98, 99, 100, 102, 105, 110 diffusion coefficient, 29, 30, 36, 37, 54, 76 dispersion, 20, 26, 28-30, 35, 40-42. See also Efficiency hydrophilic, 65 hydrophobic, 39 injection. See Injection & Autosampling on-capillary concentration, 88-90 mobility, 16, 17, 18-28, 32, 34, 40, 41, 42, 43, 48, 50, 60, 61, 65, 70, 76, 78, 87, 90, 95, 96, 101, 109 overloading. See Injection, sample loading pI, 20, 26, 50, 56, 75-77 plug length. See Injection, plug length stacking. See Injection, stacking wall interactions, 12, 29, 34, 36-39, 54-56, 66 SDS-PAGE, 74 Selectivity, 42, 47, 48, 50, 51, 53, 65, 70, 72, 96 Sensitivity. See Detection, sensitivity Separation efficiency. See Efficiency Slab gel electrophoresis, 16, 17, 59. See also SDS-PAGE Solute. See Sample Spectra. See also Detection analysis, 98, 106-109 libraries, 109 Stacking. See Injection, stacking Sulfonic acids. See Applications, sulfonic acids Surfactants, 48, 51-54, 57, 62, 65, 66, 68, 92 See also Buffer additives T Temperature, 54. See also Cooling estimation, 31-33 control, 30, 31, 32, 34, 35, 54, 86, 93, 94 effects of, 31, 32, 53, 54, 66, 86, 90, 94, 95, 96, 101, 102, 112 gradients, 30-35 Joule heating, 12, 30, 31-35, 38, 53, 55, 70, 96 thermostating, 35, 54, 65, 82, 90, 93, 95, 102 Theoretical plates (HETP), 14, 28-30 See also Efficiency Transferrin isoforms, 77 UV-Visible. See Detection, UV-Visible 134
Index V Viscosity, 24, 32, 84, 85, 86, 94, 95, 102, 112 Velocity, 17, 18, 20, 23, 24, 27, 28, 37, 43, 63, 77, 104 Voltage. See Power supply W Wall interaction. See Adsorption Wall modification. See Capillary, wall modification Z Zero injection effect, 86 Zeta potential, 20, 22, 25 Zonal electrophoresis. See CGE, CZE, MEKC Zones See Peak 135
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An introduction High performance ca
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Copyright © 2000 Agilent Technolog
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Foreword Capillary electrophoresis
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Table of content Foreword .........
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Scope The purpose of this book is t
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Introduction 1.1 High performance c
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Introduction sis, methods for on-ca
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Principles 2.1 Historical backgroun
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Principles that ion. The mobility i
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Principles the exact pI of fused si
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Principles µ EOF × 10 -4 (cm 2 /
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Principles For the analysis of smal
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Principles µ EOF ( × 10 -4 cm 2 /
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Principles Total length Effective l
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Principles Note that equation (15)
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Principles determined by the capill
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Principles Current (uA) 300 250 200
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Principles The contribution of inje
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Principles k' H N H, µm 0.001 0.58
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Principles Figure 19 Electrodispers
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Principles rapidly eluting ions, th
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Principles 44
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Modes Mode Capillary zone electroph
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Modes 3.1.1 Selectivity and the use
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Modes Name pK a Phosphate 2.12 (pK
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Modes EOF No flow Figure 22 Elimina
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Modes Absorbance 214 nm 0.05 0.04 0
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Modes Type Comment Silylation coupl
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Modes Type Result Comment Extremes
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Modes Figure 29 CZE of reversed pha
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Modes Figure 33 Ion analysis of fer
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Modes The separation mechanism of n
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Modes the stationary phase in LC. S
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Modes Amplitude 2 a) with a migrati
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Modes CGE t = 0 t > 0 Polymer matri
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Modes Crosslinked polyacrylamide, a
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Modes a) ds 500 base pairs This sam
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Modes and resolution with respect t
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Modes 3.5 Capillary isotachophoresi
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Modes 80
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Instrumentation/Operation Diode-arr
Page 84 and 85: Instrumentation/Operation Pressure
Page 86 and 87: Instrumentation/Operation If sensit
Page 88 and 89: Instrumentation/Operation Despite q
Page 90 and 91: Instrumentation/Operation T, ˚C 10
Page 92 and 93: Instrumentation/Operation 4.2.1.1 C
Page 94 and 95: Instrumentation/Operation However,
Page 96 and 97: Instrumentation/Operation Calculati
Page 98 and 99: Instrumentation/Operation Method Ma
Page 100 and 101: Instrumentation/Operation 4.3.3 Lin
Page 102 and 103: Instrumentation/Operation Area (arb
Page 104 and 105: Instrumentation/Operation 4.3.6 Ext
Page 106 and 107: Instrumentation/Operation light int
Page 108 and 109: Instrumentation/Operation 4.3.7.2 Q
Page 110 and 111: Instrumentation/Operation the capab
Page 112 and 113: Instrumentation/Operation mAU 140 1
Page 114 and 115: Abbreviations Abbreviation Full Nam
Page 120 and 121: References/bibliography References
Page 122 and 123: References/bibliography 15 W.C. Bru
Page 124 and 125: References/bibliography 29 R.L. Chi
Page 126 and 127: References/bibliography 126
Page 128 and 129: Index A Absorption. See Detection,
Page 130 and 131: Index D DAD. See Detection, diode-a
Page 132 and 133: Index I Ionic strength , 22, 25, 26
Page 136: www.agilent.com/chem Copyright © 2
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