High performance capillary electrophoresis - T.E.A.M.
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High performance capillary electrophoresis - T.E.A.M.

High performance capillary electrophoresis - T.E.A.M. High performance capillary electrophoresis - T.E.A.M.

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Index D DAD. See Detection, diode-array Data collection rate. See Detection, data collection rate Deoxyribonucleic acid (DNA). 29, 54, 70, 71, 73, 74 oligonucleotides, 72, 73 restriction fragments, 73 Detection absorption 97-99, 107. See also Detection UV-Visible bubble cell, 104, 105 calibration curve, 94, 100 data collection rate, 103 design, 97, 100, 101 diode-array, 81, 105-109 extended light path capillary, 104, 105 indirect, 61, 98 limits, 69, 97-100, 106 linear detection range. 97, 100, 101, 104, 106 mass spectrometry, 98 methods, 97, 98 multisignal, 107 noise, 86, 99-91, 106 on-capillary, 10, 27, 50, 97 optical window, 82, 91. See also Detection, bubble cell response time, 103 sensitivity, 88, 97-99, 100, 104-106 slit size, 97, 100, 101 stray light, 94, 100, 101 UV-Visible, 88, 97, 98, 99 UV wavelengths, 48, 99, 105, 106, 107, 108 Diffusion, coefficient, 29, 36, 37, 54, 76 longitudinal, 29 Diode-array. See Detection, diode-array Dispersion. See Sample, dispersion Drugs, 52, 68. See also Applications, illicit drugs & pharmaceutical DNA. See Deoxyribonucleic acid E Effective mobility, 19, 27 Efficiency, 10, 16, 24, 25, 28-37, 42, 43, 52, 54, 60, 65, 72, 93, 97, 103, 104, 109, 112. See also Sample, dispersion Electrodispersion, 30, 31, 40-42. See also Sample, dispersion Electric field, 10, 16, 17, 19-21, 24, 28, 31, 65, 70, 75, 76, 78, 91. See also Field programming Electrolytes. See Buffers Electro-osmotic flow (EOF), 19-27, 43, 47–53, 56–60, 61–68,76, 87, 89, 93–96, 111. anions, 41, 47 cations, 47, 61 control, 24-26, 49, 72, 110, 111 direction, 93, 94. See also EOF, reversal effect on sample loading, 87, 89 effect on resolution, 43, 64, 65 in Teflon capillaries, 20, 91 internal standard, 96 130

Index magnitude, 22, 21, 93 marker, 27 micelle migration, 62, 63, 64-67 mobility, 20, 27, 34, 87, 96 normalization of, 93, 95 pH dependence, 20, 22, 38, 48, 50, 92, 111 process, 21 profile, 21, 22 reduction, 72, 76 reversal, 52, 56, 61, 57 temperature, 54 velocity, 17, 18, 20, 23, 27, 34, 42, 47, 63, 78, 104 Environmental analysis. See Applications, environmental EOF. See Electroosmotic flow F Field amplified sample injection, 88-90 Field programming, 94, 110, 111 Focusing, 40, 59, 75-77, 89 Fraction collection, 91, 94, 110, 111 G Gels, 16, 59, 69-74, 88 Glycoproteins, 58, 59 H Hagen-Poiseuille equation, 84 HETP, height equivalent to a theoretical plate, 28. See also Efficiency Heat removal. See Cooling Historical development, 16, 17 Agilent CE system, 82 Hydrophobic interaction. See Adsorption Injection, automatic sampling. See Autosampling effect on efficiency, 36 effect on resolution, 35 electrokinetic, 84, 87, 88 field amplified, 88-90 hydrodynamic, 72, 83-89 plug length, 33, 34, 35, 35-36, 82, 83, 86 quantity loaded, 82-86 reproducibility, 86 sample loading, 35, 36, 82, 83, 86. See also, Injection, siphoning sample concentration, 78, 92. See also, Injection, stacking siphoning, 93, 85, 86 stacking, 88-90, 103 volumes, 36, 76, 82, 83, 84-86, 96 zero injection effect, 86 Inorganic ions. See Applications, inorganic ions Internal standard, 96, 111 Instrument design, 82. See also Detection, design 131

Index<br />

magnitude, 22, 21, 93<br />

marker, 27<br />

micelle migration, 62, 63, 64-67<br />

mobility, 20, 27, 34, 87, 96<br />

normalization of, 93, 95<br />

pH dependence, 20, 22, 38, 48, 50, 92, 111<br />

process, 21<br />

profile, 21, 22<br />

reduction, 72, 76<br />

reversal, 52, 56, 61, 57<br />

temperature, 54<br />

velocity, 17, 18, 20, 23, 27, 34, 42, 47, 63, 78, 104<br />

Environmental analysis. See Applications, environmental<br />

EOF. See Electroosmotic flow<br />

F<br />

Field amplified sample injection, 88-90<br />

Field programming, 94, 110, 111<br />

Focusing, 40, 59, 75-77, 89<br />

Fraction collection, 91, 94, 110, 111<br />

G<br />

Gels, 16, 59, 69-74, 88<br />

Glycoproteins, 58, 59<br />

H<br />

Hagen-Poiseuille equation, 84<br />

HETP, height equivalent to a theoretical plate, 28. See also Efficiency<br />

Heat removal. See Cooling<br />

Historical development, 16, 17<br />

Agilent CE system, 82<br />

Hydrophobic interaction. See Adsorption<br />

Injection,<br />

automatic sampling. See Autosampling<br />

effect on efficiency, 36<br />

effect on resolution, 35<br />

electrokinetic, 84, 87, 88<br />

field amplified, 88-90<br />

hydrodynamic, 72, 83-89<br />

plug length, 33, 34, 35, 35-36, 82, 83, 86<br />

quantity loaded, 82-86<br />

reproducibility, 86<br />

sample loading, 35, 36, 82, 83, 86. See also, Injection, siphoning<br />

sample concentration, 78, 92. See also, Injection, stacking<br />

siphoning, 93, 85, 86<br />

stacking, 88-90, 103<br />

volumes, 36, 76, 82, 83, 84-86, 96<br />

zero injection effect, 86<br />

Inorganic ions. See Applications, inorganic ions<br />

Internal standard, 96, 111<br />

Instrument design, 82. See also Detection, design<br />

131

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