High performance capillary electrophoresis - T.E.A.M.

Index
D
DAD. See Detection, diode-array
Data collection rate. See Detection, data collection rate
Deoxyribonucleic acid (DNA). 29, 54, 70, 71, 73, 74
oligonucleotides, 72, 73
restriction fragments, 73
Detection
absorption 97-99, 107. See also Detection UV-Visible
bubble cell, 104, 105
calibration curve, 94, 100
data collection rate, 103
design, 97, 100, 101
diode-array, 81, 105-109
extended light path capillary, 104, 105
indirect, 61, 98
limits, 69, 97-100, 106
linear detection range. 97, 100, 101, 104, 106
mass spectrometry, 98
methods, 97, 98
multisignal, 107
noise, 86, 99-91, 106
on-capillary, 10, 27, 50, 97
optical window, 82, 91. See also Detection, bubble cell
response time, 103
sensitivity, 88, 97-99, 100, 104-106
slit size, 97, 100, 101
stray light, 94, 100, 101
UV-Visible, 88, 97, 98, 99
UV wavelengths, 48, 99, 105, 106, 107, 108
Diffusion,
coefficient, 29, 36, 37, 54, 76
longitudinal, 29
Diode-array. See Detection, diode-array
Dispersion. See Sample, dispersion
Drugs, 52, 68. See also Applications, illicit drugs & pharmaceutical
DNA. See Deoxyribonucleic acid
E
Effective mobility, 19, 27
Efficiency, 10, 16, 24, 25, 28-37, 42, 43, 52, 54, 60, 65, 72, 93, 97, 103, 104, 109, 112.
See also Sample, dispersion
Electrodispersion, 30, 31, 40-42. See also Sample, dispersion
Electric field, 10, 16, 17, 19-21, 24, 28, 31, 65, 70, 75, 76, 78, 91. See also Field
programming
Electrolytes. See Buffers
Electro-osmotic flow (EOF), 19-27, 43, 47–53, 56–60, 61–68,76, 87, 89, 93–96, 111.
anions, 41, 47
cations, 47, 61
control, 24-26, 49, 72, 110, 111
direction, 93, 94. See also EOF, reversal
effect on sample loading, 87, 89
effect on resolution, 43, 64, 65
in Teflon capillaries, 20, 91
internal standard, 96
130