High performance capillary electrophoresis - T.E.A.M.
High performance capillary electrophoresis - T.E.A.M.
High performance capillary electrophoresis - T.E.A.M.
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Instrumentation/Operation<br />
4.3.3 Linear detection range<br />
Instrumental deviations from Beer’s law ultimately limit<br />
quantitative analysis. At high analyte concentrations, the<br />
deviation usually takes the form of decreased slope of the<br />
calibration curve (figure 57). The linear detection range is<br />
mainly limited by stray light reaching the detector. Ideally,<br />
all light should pass through the center of the <strong>capillary</strong>, not<br />
through the wall. Note that the linear detection range is<br />
significantly lower than that observed in LC (0.4 to 0.7 AU<br />
in CE versus 1.2 to 1.5 AU in LC) due to the small size and<br />
curvature of the <strong>capillary</strong>.<br />
Absorbance<br />
Capillary wall<br />
Light<br />
Baseline noise<br />
Linear detection<br />
range<br />
Concentration<br />
Absorbance<br />
Capillary wall<br />
Light<br />
Baseline noise<br />
Figure 57<br />
Dependence of linear detection range and<br />
baseline noise on light path through the<br />
<strong>capillary</strong><br />
Linear detection<br />
range<br />
Concentration<br />
100
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