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RA 00110.pdf - OAR@ICRISAT

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Figure 10. Autoflourescence microscopy of pearl millet sections embedded in glycol methacrylate. A - D .<br />

Autoflourescence. A. 4 0 X , bronze millet. B. 100X, bronze millet. C. 4 0 X , blue-grey millet. D. 100X, blue-grey<br />

millet. E. Congo Red, 100X, bronze millet. F. Autoflourescence with Acid Fuschin dye, 100X, tan millet.<br />

(p = peripheral endosperm; a = aleurone; s - seed coat; t - tube cells; c = cross cells; e - epicarp cells; pb =<br />

protein bodies; sg = starch granules).<br />

endosperm and partial chunks of endosperm cells<br />

from the corneous and peripheral endosperm.<br />

There may also be pieces of bran left in the fraction<br />

as well as chunks of germ tissue.<br />

The germ of pearl millet is about 17% of the total<br />

kernel mass (Table 3) (Abdelrahman et al. 1984),<br />

and contains about 25% lipid, 20% protein, and<br />

most of the phytin, vitamins, and enzymes. The protein<br />

content of the germ is of high quality with high<br />

levels of lysine, tryptophan, and theonine. The germ<br />

is firmly embedded inside the pericarp and endosperm<br />

so it is difficult to remove completely by milling<br />

processes. Many cereals contain phytin and<br />

nicotinic acid in the aleurone and germ, but pearl<br />

millet appears to contain these materials mainly in<br />

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