Biophysical studies of membrane proteins/peptides. Interaction with ...

2.2. – Peptides as models
2.3. – Amphipatic helix
2.4. – Peptide partitioning to the membrane
2.5. – Anchoring of transmembrane domains
2.6. – Lipid-protein hydrophobic mismatch
2.7. – Transmembrane protein-lipid interface
2.8. – Lipid selectivity at protein interfaces
2.9. – Lipid phase preferential partition of membrane proteins
2.10. – Lipid sorting by proteins and formation of lipid domains
2.11. – Lipid-mediation of protein-protein interactions
25
26
28
30
31
35
37
41
43
44
II – PROTEIN-PROTEIN AND PROTEIN-LIPID INTERACTIONS
OF M13 MAJOR COAT PROTEIN
1. – Introduction
2. – Dependence of M13 Major Coat Protein Oligomerization and Lateral
Segregation on Bilayer Composition
3. – Quantification of Protein-Lipid Selectivity Using FRET: Application to
the M13 MCP
47
47
53
67
III – BINDING OF INHIBITORS TO A PUTATIVE BINDING
DOMAIN OF V-ATPase
1. – Introduction
2. – Interaction of the Indole Class of Vacuolar H + -ATPase Inhibitors with
Lipid Bilayers
3. – Binding Assays of Inhibitors Towards Selected V-ATPase Domains
79
79
83
95
IV – BINDING OF A QUINOLONE ANTIBIOTIC TO BACTERIAL
PORIN OmpF
1. – Introduction
2. – Ciprofloxacin Interactions With Bacterial Protein OmpF: Modelling of
FRET From a Multi-Tryptophan Protein Trimer
107
107
109