Biophysical studies of membrane proteins/peptides. Interaction with ...

More documents

Recommendations

Info

Ao Pedro e Hugo, amigos de longa data, por me conseguirem arrancar de casa e sempre ajudarem a manter a vida em perspectiva. E finalmente gostava de agradecer aos meus irmãos e meus pais, pelo exemplo, encorajamento e amor, sem os quais esta tese não seria certamente produzida.
CONTENTS CONTENTS Prefácio Contents Abbreviations and Symbol List Resumo Palavras-Chave Abstract Keywords Sinopse Outline I - INTRODUCTION I V IX XI XI XIII XIII XV XXI 1 1.- Biomembranes 1.1. – Function and architecture of biomembranes 1.2. – Molecular composition of biomembranes 1.2.1. – Lipid composition 1.2.1.1. – Glycerolipids 1.2.1.2. – Sphingolipids 1.2.1.3. – Glycolipids 1.2.1.4. – Sterols 1.3. – Lipid asymmetry across the bilayer 1.4. – Lipid structure and curvature 1.5. – Lamellar phase transitions in lipid bilayers 1.6. – Membrane thickness 1.7. – Lateral heterogeneity in lipid bilayer 1.8. – Membrane proteins 1.9. – Lateral dynamics in biomembranes 1.10. – Membrane model systems 1 1 3 3 3 5 6 6 7 8 10 12 14 17 20 21 2. – Lipid-Protein Interactions 2.1. – Membrane protein reconstitution 23 23 v
Page 1: UNIVERSIDADE TÉCNICA DE LISBOA INS
Page 4 and 5: Fernandes, F., Loura, L. M. S., Fed
Page 8 and 9: 2.2. - Peptides as models 2.3. - Am
Page 11 and 12: ABBREVIATIONS AND SYMBOL LIST ABBRE
Page 13: RESUMO RESUMO As biomembranas são
Page 17 and 18: SINOPSE SINOPSE Nas últimas duas d
Page 19 and 20: SINOPSE podem fornecer informação
Page 21 and 22: SINOPSE aceitantes. Dadores mais pr
Page 23 and 24: OUTLINE OUTLINE The last two decade
Page 25 and 26: OUTLINE membranes with a distributi
Page 27: OUTLINE BAR domains (tubulation of
Page 30 and 31: ion diffusion, as the energy requir
Page 32 and 33: acid. If no more groups are linked
Page 34 and 35: sphingomyelin, the most abundant sp
Page 36 and 37: signal for neighbouring cells to ph
Page 38 and 39: functional role in process such as
Page 40 and 41: in the L α phase, while lateral di
Page 42 and 43: 1.7. Lateral heterogeneity in lipid
Page 44 and 45: the vesicle through bilayer deforma
Page 46 and 47: Figure I.9 - Depiction of the sever
Page 48 and 49: Figure I.11 - Experimentally obtain
Page 50 and 51: drying into a film and ressuspensio
Page 52 and 53: deactivating agents. Zwitterionic d
Page 54 and 55: amphipatic helices (see Section 2.3
Page 56 and 57:
size corresponding to the hydrophob
Page 58 and 59:
changes abruptly in the interfacial
Page 60 and 61:
thickness of the bilayer (Section 1
Page 62 and 63:
some α-helical membrane proteins a
Page 64 and 65:
The formation of a lipid population
Page 66 and 67:
homogeneous distribution of lipids
Page 68 and 69:
Figure I.20 - Relative binding cons
Page 70 and 71:
2.9. Lipid phase preferential parti
Page 72 and 73:
In Figure I.21, theoretical simulat
Page 75 and 76:
PROTEIN-PROTEIN AND PROTEIN-LIPID I
Page 77 and 78:
Page 79:
Page 83 and 84:
2430 Biophysical Journal Volume 85
Page 85 and 86:
2432 Fernandes et al. Coat protein
Page 87 and 88:
2434 Fernandes et al. leads to an a
Page 89 and 90:
2436 Fernandes et al. FIGURE 4 (A)
Page 91 and 92:
2438 Fernandes et al. section of th
Page 93 and 94:
2440 Fernandes et al. tein oligomer
Page 95:
QUANTIFICATION OF PROTEIN-LIPID SEL
Page 98 and 99:
FRET Study of Protein-Lipid Selecti
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 107 and 108:
BINDING OF INHIBITORS TO A PUTATIVE
Page 109 and 110:
BINDING OF INHIBITORS TO A PUTATIVE
Page 111:
INTERACTION OF THE INDOLE CLASS OF
Page 114 and 115:
5272 Biochemistry, Vol. 45, No. 16,
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 123:
BINDING ASSAYS OF INHIBITORS TOWARD
Page 126 and 127:
1778 F. Fernandes et al. / Biochimi
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
apparently the most significant as
Page 138 and 139:
110
Page 140 and 141:
Introduction Quinolones are broad-s
Page 142 and 143:
[9-12]. Having this into considerat
Page 144 and 145:
any case, very similar, probably wi
Page 146 and 147:
placement of the protein around the
Page 148 and 149:
⎛ II t ⎛ R ⎞ 0 ρ () t = exp
Page 150 and 151:
APPENDIX - Derivation of the FRET r
Page 152 and 153:
2 2w J ( t) = '2 R − R + w / 1
Page 154 and 155:
[14] L. Plançon, M. Chami, L. Lete
Page 156 and 157:
acceptors) (Eqs. 4-6) (⋅-⋅-⋅)
Page 158 and 159:
FIGURE 1A FIGURE 1B 130
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
136
Page 166 and 167:
dynamin. Soon after, the same group
Page 168 and 169:
140
Page 170 and 171:
142
Page 172 and 173:
Introduction Control of membrane re
Page 174 and 175:
Steady-state fluorescence measureme
Page 176 and 177:
Rho-DOPE (acceptor). The increase o
Page 178 and 179:
where η is the viscosity of the me
Page 180 and 181:
ound conformation of the BAR domain
Page 182 and 183:
19. Fernandes, F., L. M. S. Loura,
Page 184 and 185:
Figure Legends Fig.1: N-terminal am
Page 186 and 187:
FIG. 1A FIG.1B 17
Page 188 and 189:
FIG. 3A FIG. 3B 19
Page 190 and 191:
FIG.5A 21
Page 192 and 193:
FIG 6. 23
Page 194 and 195:
FIG. 8 A B 25
Page 196 and 197:
The signalling functions of PI(4,5)
Page 198 and 199:
172
Page 200 and 201:
174
Page 202 and 203:
zoxadiazol (NBD)-PC were obtained f
Page 204 and 205:
Fig. 3. Fluorescence decays of diph
Page 206 and 207:
CONCLUSIONS VII CONCLUSIONS Chapter
Page 208 and 209:
CONCLUSIONS constants recovered by
Page 210 and 211:
CONCLUSIONS Chapter IV ‐ Binding
Page 212 and 213:
CONCLUSIONS Chapter VI - Clustering
Page 214 and 215:
FINAL CONSIDERATIONS AND PROSPECTS
Page 216 and 217:
BIBLIOGRAPHY IX BIBLIOGRAPHY Albert
Page 218 and 219:
BIBLIOGRAPHY Phosphatidylinositol 4
Page 220 and 221:
BIBLIOGRAPHY Glaubitz, C., Grobner,
Page 222 and 223:
BIBLIOGRAPHY Koehorst, R. B. M., Sp
Page 224 and 225:
BIBLIOGRAPHY Mishra, V. K., Palguna
Page 226 and 227:
BIBLIOGRAPHY Pluschke, G., Hirota,
Page 228 and 229:
BIBLIOGRAPHY Sperotto, M. M., and M
Page 230:
BIBLIOGRAPHY Williamson, I. M., Alv
show all

Biophysical studies of membrane proteins/peptides. Interaction with ...

Create successful ePaper yourself

Delete template?

Save as template?