Biophysical studies of membrane proteins/peptides. Interaction with ...

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BIBLIOGRAPHY Williamson, I. M., Alvis, S. J., East, J. M., and Lee, A. G. (2002). Interaction of Phospholipids with the Potassium Channel KscA. Biophys. J. 83: 2026-2038. Wilson, P. T., Lentz, T.L., and Hawrot, E. (1985). Determination of the Primary Amino Acid Sequence Specifying the Alpha-Bungarotoxin Binding Site on the Alpha-Subunit of the Acetylcholine Receptor From Torpedo Californica. Proc. Natl. Acad. Sci. USA 82: 8790-8794. Wimley, W. C., and White, S. H. (1996). Experimentally Determined Hydrophobicity Scale for Proteins at Membrane Interfaces. Nat. Struct. Biol. 3: 842-848. Wimley, W.C., Creamer, T.P., and White, S.H. (1996) Solvation Energies of Amino Acid Residues and Backbone in a Family of Host Guest Pentapeptides. Biochemistry 35: 5109-5124. Yau, W.-M., Wimley, W. C., Gawrisch, K., and White, S. H. (1998). The Preference of Tryptophan for Membrane Interfaces. Biochemistry 37: 14713-14718. Yeagle, P. L. (1993). The Membranes of Cells. 2 nd Ed. Academic Press, San Diego, USA. Yeagle, P. L., Bennet, M., Lemaître, V., and Watts, A. (2007). Transmembrane Helices of Membrane Proteins May Flex to Satisfy Hydrophobic Mismatch. Biochim. Biophys. Acta 1768: 530-537. Yokoyama, K., Nakano, M., Imamura, H., Yoshida, M., and Tamakoshi, M. (2003). Rotation of the Proteolipid Ring in the V-ATPase. J. Biol. Chem. 278: 24255-24258. Yoshida, Y., Kinuta, M., Abe, T., Liang, S., Araki, K., Cremona, O., Di Paolo, G., Moriyama, Y., Yasuda, T., De Camilli, P., and Takei, K. (2004). The Stimulatory Action of Amphiphysin on Dynamin Function is Dependent on Lipid Bilayer Curvature. EMBO J. 23: 3483-3491. Zimmerberg, J. (2000). Are the Curves in All the Right Places Traffic 1: 366-368. Zimmerberg, J., and Kozlov, M. M. (2006). How Proteins Produce Cellular Membrane Curvature. Nat. Rev. Mol. Cell Biol. 7: 9-19. 205
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UNIVERSIDADE TÉCNICA DE LISBOA INS
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Fernandes, F., Loura, L. M. S., Fed
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Ao Pedro e Hugo, amigos de longa da
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2.2. - Peptides as models 2.3. - Am
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ABBREVIATIONS AND SYMBOL LIST ABBRE
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RESUMO RESUMO As biomembranas são
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SINOPSE SINOPSE Nas últimas duas d
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SINOPSE podem fornecer informação
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SINOPSE aceitantes. Dadores mais pr
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OUTLINE OUTLINE The last two decade
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OUTLINE membranes with a distributi
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OUTLINE BAR domains (tubulation of
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ion diffusion, as the energy requir
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acid. If no more groups are linked
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sphingomyelin, the most abundant sp
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signal for neighbouring cells to ph
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functional role in process such as
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in the L α phase, while lateral di
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1.7. Lateral heterogeneity in lipid
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the vesicle through bilayer deforma
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Figure I.9 - Depiction of the sever
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Figure I.11 - Experimentally obtain
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drying into a film and ressuspensio
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deactivating agents. Zwitterionic d
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amphipatic helices (see Section 2.3
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size corresponding to the hydrophob
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changes abruptly in the interfacial
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thickness of the bilayer (Section 1
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some α-helical membrane proteins a
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The formation of a lipid population
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homogeneous distribution of lipids
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Figure I.20 - Relative binding cons
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2.9. Lipid phase preferential parti
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In Figure I.21, theoretical simulat
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PROTEIN-PROTEIN AND PROTEIN-LIPID I
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2430 Biophysical Journal Volume 85
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2432 Fernandes et al. Coat protein
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2434 Fernandes et al. leads to an a
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2436 Fernandes et al. FIGURE 4 (A)
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2438 Fernandes et al. section of th
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2440 Fernandes et al. tein oligomer
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QUANTIFICATION OF PROTEIN-LIPID SEL
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FRET Study of Protein-Lipid Selecti
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BINDING OF INHIBITORS TO A PUTATIVE
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BINDING OF INHIBITORS TO A PUTATIVE
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INTERACTION OF THE INDOLE CLASS OF
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5272 Biochemistry, Vol. 45, No. 16,
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BINDING ASSAYS OF INHIBITORS TOWARD
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1778 F. Fernandes et al. / Biochimi
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apparently the most significant as
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110
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Introduction Quinolones are broad-s
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[9-12]. Having this into considerat
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any case, very similar, probably wi
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placement of the protein around the
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⎛ II t ⎛ R ⎞ 0 ρ () t = exp
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APPENDIX - Derivation of the FRET r
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2 2w J ( t) = '2 R − R + w / 1
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[14] L. Plançon, M. Chami, L. Lete
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acceptors) (Eqs. 4-6) (⋅-⋅-⋅)
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FIGURE 1A FIGURE 1B 130
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136
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dynamin. Soon after, the same group
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140
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142
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Introduction Control of membrane re
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Steady-state fluorescence measureme
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Rho-DOPE (acceptor). The increase o
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where η is the viscosity of the me
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Page 184 and 185: Figure Legends Fig.1: N-terminal am
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Page 188 and 189: FIG. 3A FIG. 3B 19
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Page 196 and 197: The signalling functions of PI(4,5)
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Page 202 and 203: zoxadiazol (NBD)-PC were obtained f
Page 204 and 205: Fig. 3. Fluorescence decays of diph
Page 206 and 207: CONCLUSIONS VII CONCLUSIONS Chapter
Page 208 and 209: CONCLUSIONS constants recovered by
Page 210 and 211: CONCLUSIONS Chapter IV ‐ Binding
Page 212 and 213: CONCLUSIONS Chapter VI - Clustering
Page 214 and 215: FINAL CONSIDERATIONS AND PROSPECTS
Page 216 and 217: BIBLIOGRAPHY IX BIBLIOGRAPHY Albert
Page 218 and 219: BIBLIOGRAPHY Phosphatidylinositol 4
Page 220 and 221: BIBLIOGRAPHY Glaubitz, C., Grobner,
Page 222 and 223: BIBLIOGRAPHY Koehorst, R. B. M., Sp
Page 224 and 225: BIBLIOGRAPHY Mishra, V. K., Palguna
Page 226 and 227: BIBLIOGRAPHY Pluschke, G., Hirota,
Page 228 and 229: BIBLIOGRAPHY Sperotto, M. M., and M
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