ABI PRISM 7000 Sequence Detection Systems Relative ...

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Index software, RQ Study 49 Spectra view 28 standard curves 2 standards xvi starting an RQ plate run 26 study, RQ. See RQ studies SYBR Green I dye chemistry 11 symbols xi workflow experiment design 7 reverse transcription 15 RQ experiment overview 2 RQ plates 19 RQ studies 33 workstation safety xv T TaqMan chemistry 11 TaqMan Universal PCR Master Mix 20 target associating with detectors 22, 24 definition 9 tasks. See detector tasks Technical Communications, contacting viii Technical Support, contacting viii, 2 template documents 24 text conventions vii thermal cycling conditions default for PCR 25 one-step RT-PCR 25 specifying 26 thermal cycling parameters for plates added to a study 34 RT using High Capacity cDNA Archive kit 16 threshold cycle definition 27 setting for RQ studies 35 Training, obtaining information about viii training, obtaining information about 2 types of assays 1 U ultraviolet light xv uracil-N-glycosylase 12 US safety standards xvi V VIC dye 13 W WARNING, description x Well Information tab 36 Well Inspector dialog box 23 wells, replicate 9 54 <strong>Relative</strong> Quantification Getting Started Guide for <strong>7000</strong> v1.1 SAMPLE DOCUMENT September 26, 2003 3:14 pm, RQGSG_IX.fm
DRAFT September 26, 2003 3:17 pm, GSG_BackCover.fm Back Cover
Page 1 and 2:
5′ cDNA Reverse Primer Oligo d(T)
Page 3 and 4:
Primer Extended on mRNA 5′ 3′ 5
Page 5 and 6:
Chapter 5 Performing an RQ Study 33
Page 7 and 8:
Preface How to Use This Guide Purpo
Page 9 and 10:
Safety and EMC Compliance Informati
Page 11 and 12:
Symbols on Instruments Electrical S
Page 13 and 14:
Chemical Safety Chemical Hazard War
Page 15 and 16:
Physical Hazard Safety Ultraviolet
Page 17 and 18:
Safety and Electromagnetic Compatib
Page 19 and 20:
Introduction 1 About the 7000 SDS I
Page 21 and 22: About the Sample RQ Experiment Desc
Page 23 and 24: Materials and Equipment Description
Page 25 and 26: Primer Extended on mRNA 5′ 3′ 5
Page 27 and 28: Specifying the Components of an RQ
Page 29 and 30: Selecting the Sequence Detection Ch
Page 31 and 32: Choosing the Probes and Primers Abo
Page 35 and 36: 5′ 3′ 5′ cDNA Reverse Primer
Page 39 and 40: Preparing the PCR Master Mix Preven
Page 41 and 42: Creating an RQ Plate Document Detec
Page 43 and 44: Specifying Thermal Cycling Conditio
Page 45 and 46: Analyzing and Viewing RQ Plate Data
Page 47 and 48: Analyzing and Viewing RQ Plate Data
Page 49 and 50: Exporting RQ Plate Documents Reanal
Page 53 and 54: Creating an RQ Study Document 5. Co
Page 55 and 56: Analyzing and Viewing the Results o
Page 57 and 58: Analyzing and Viewing the Results o
Page 59 and 60: Omitting Samples from a Study Omitt
Page 61 and 62: Exporting RQ Study Results Exportin
Page 63 and 64: References Kwok, S. and Higuchi, R.
Page 65 and 66: Creating Detectors A Before you can
Page 67 and 68: Upgrading the 7000 Software with RQ
Page 69 and 70: Index SAMPLE DOCUMENT September 26,
Page 71: Index SAMPLE DOCUMENT September 26,
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