ABI PRISM 7000 Sequence Detection Systems Relative ...

Preface
How to Use This Guide
Purpose of This
Guide
Assumptions
This manual is written for principal investigators and laboratory staff who conduct
relative quantification studies for gene expression using the ABI PRISM ® 7000 Sequence
Detection System (7000 SDS instrument).
This guide assumes that you have:
• Familiarity with Microsoft ® Windows ® 2000 operating system.
• Knowledge of general techniques for handling DNA samples and preparing them
for electrophoresis.
• A general understanding of hard drives and data storage, file transfers, and copying
and pasting.
If you want to integrate the ABI PRISM ® 7000 Sequence Detection System into your
existing laboratory data flow system, you need networking experience.
Text Conventions
This guide uses the following conventions:
DRAFT
September 26, 2003 3:14 pm, RQGSG_Preface.fm
User Attention
Words
• Bold indicates user action. For example:
Type 0, then press Enter for each of the remaining fields.
• Italic text indicates new or important words and is also used for emphasis. For
example:
Before analyzing, always prepare fresh matrix.
• A right arrow bracket (>) separates successive commands you select from a dropdown
or shortcut menu. For example:
Select File > Open > Spot Set.
Right-click the sample row, then select View Filter > View All Runs.
Two user attention words appear in Applied Biosystems user documentation. Each word
implies a particular level of observation or action as described below:
Note: Provides information that may be of interest or help but is not critical to the use of
the product.
IMPORTANT! Provides information that is necessary for proper instrument operation,
accurate chemistry kit use, or safe use of a chemical.
Relative Quantification Getting Started Guide for 7000 v1.1
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