ABI PRISM 7000 Sequence Detection Systems Relative ...

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Chapter 5 Performing an RQ Study Exporting RQ Study Results DRAFT September 26, 2003 3:14 pm, C5_RQStudy.fm Notes 44 Relative Quantification Getting Started Guide for 7000 v1.1
References Kwok, S. and Higuchi, R. 1989. Avoiding false positives with PCR. Nature 339:237–238. Mullis, K.B. and Faloona, F.A. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335–350. Livak, K.J., and Schmittgen, T.D. 2001. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 –∆∆C T Method. Methods 25:402–408. Saiki, R.K., Scharf, S., Faloona, F., et al. 1985. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354. DRAFT September 26, 2003 3:17 pm, RQGSG_References.fm Relative Quantification Getting Started Guide for 7000 v1.1 45
Page 1 and 2:
5′ cDNA Reverse Primer Oligo d(T)
Page 3 and 4:
Primer Extended on mRNA 5′ 3′ 5
Page 5 and 6:
Chapter 5 Performing an RQ Study 33
Page 7 and 8:
Preface How to Use This Guide Purpo
Page 9 and 10:
Safety and EMC Compliance Informati
Page 11 and 12: Symbols on Instruments Electrical S
Page 13 and 14: Chemical Safety Chemical Hazard War
Page 15 and 16: Physical Hazard Safety Ultraviolet
Page 17 and 18: Safety and Electromagnetic Compatib
Page 19 and 20: Introduction 1 About the 7000 SDS I
Page 21 and 22: About the Sample RQ Experiment Desc
Page 23 and 24: Materials and Equipment Description
Page 25 and 26: Primer Extended on mRNA 5′ 3′ 5
Page 27 and 28: Specifying the Components of an RQ
Page 29 and 30: Selecting the Sequence Detection Ch
Page 31 and 32: Choosing the Probes and Primers Abo
Page 35 and 36: 5′ 3′ 5′ cDNA Reverse Primer
Page 39 and 40: Preparing the PCR Master Mix Preven
Page 41 and 42: Creating an RQ Plate Document Detec
Page 43 and 44: Specifying Thermal Cycling Conditio
Page 45 and 46: Analyzing and Viewing RQ Plate Data
Page 47 and 48: Analyzing and Viewing RQ Plate Data
Page 49 and 50: Exporting RQ Plate Documents Reanal
Page 53 and 54: Creating an RQ Study Document 5. Co
Page 55 and 56: Analyzing and Viewing the Results o
Page 57 and 58: Analyzing and Viewing the Results o
Page 59 and 60: Omitting Samples from a Study Omitt
Page 61: Exporting RQ Study Results Exportin
Page 65 and 66: Creating Detectors A Before you can
Page 67 and 68: Upgrading the 7000 Software with RQ
Page 69 and 70: Index SAMPLE DOCUMENT September 26,
Page 71 and 72: Index SAMPLE DOCUMENT September 26,
Page 73 and 74: DRAFT September 26, 2003 3:17 pm, G
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