ABI PRISM 7000 Sequence Detection Systems Relative ...

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Chapter 5 Performing an RQ Study Omitting Samples from a Study 6. Do one of the following: • If outliers are present, select the Well Information tab, find the outlying sample, and select the Omit check box for the sample. • If outliers are not present, go to step 7. 7. Repeat steps 5 and 6 to screen the remaining replicate groups. 8. Select Analysis > Analyze ( ) to reanalyze the run without the outlying data. 9. Repeat steps 3 to 8 for other detectors you want to screen. Select the Omit check box. The outlier is removed during analysis. DRAFT September 26, 2003 3:14 pm, C5_RQStudy.fm Notes 42 Relative Quantification Getting Started Guide for 7000 v1.1
Exporting RQ Study Results Exporting RQ Study Results 1. Select File > Export > Results, then select the data type to export: • Sample Summary (*.csv) • Well Information (*.csv) • Both (*.csv) 2. Enter a file name for the export file. Note: The name of the dialog box depends on the type of data you want to export. 3. Click Save. DRAFT September 26, 2003 3:14 pm, C5_RQStudy.fm 5 Notes Relative Quantification Getting Started Guide for 7000 v1.1 43
Page 1 and 2:
5′ cDNA Reverse Primer Oligo d(T)
Page 3 and 4:
Primer Extended on mRNA 5′ 3′ 5
Page 5 and 6:
Chapter 5 Performing an RQ Study 33
Page 7 and 8:
Preface How to Use This Guide Purpo
Page 9 and 10: Safety and EMC Compliance Informati
Page 11 and 12: Symbols on Instruments Electrical S
Page 13 and 14: Chemical Safety Chemical Hazard War
Page 15 and 16: Physical Hazard Safety Ultraviolet
Page 17 and 18: Safety and Electromagnetic Compatib
Page 19 and 20: Introduction 1 About the 7000 SDS I
Page 21 and 22: About the Sample RQ Experiment Desc
Page 23 and 24: Materials and Equipment Description
Page 25 and 26: Primer Extended on mRNA 5′ 3′ 5
Page 27 and 28: Specifying the Components of an RQ
Page 29 and 30: Selecting the Sequence Detection Ch
Page 31 and 32: Choosing the Probes and Primers Abo
Page 35 and 36: 5′ 3′ 5′ cDNA Reverse Primer
Page 39 and 40: Preparing the PCR Master Mix Preven
Page 41 and 42: Creating an RQ Plate Document Detec
Page 43 and 44: Specifying Thermal Cycling Conditio
Page 45 and 46: Analyzing and Viewing RQ Plate Data
Page 47 and 48: Analyzing and Viewing RQ Plate Data
Page 49 and 50: Exporting RQ Plate Documents Reanal
Page 53 and 54: Creating an RQ Study Document 5. Co
Page 55 and 56: Analyzing and Viewing the Results o
Page 57 and 58: Analyzing and Viewing the Results o
Page 59: Omitting Samples from a Study Omitt
Page 63 and 64: References Kwok, S. and Higuchi, R.
Page 65 and 66: Creating Detectors A Before you can
Page 67 and 68: Upgrading the 7000 Software with RQ
Page 69 and 70: Index SAMPLE DOCUMENT September 26,
Page 71 and 72: Index SAMPLE DOCUMENT September 26,
Page 73 and 74: DRAFT September 26, 2003 3:17 pm, G
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