ABI PRISM 7000 Sequence Detection Systems Relative ...
ABI PRISM 7000 Sequence Detection Systems Relative ...
ABI PRISM 7000 Sequence Detection Systems Relative ...
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Chapter 5 Performing an RQ Study<br />
Analyzing and Viewing the Results of the RQ Study<br />
Viewing Gene<br />
Expression Plots<br />
Gene Expression plots show the expression level or fold-difference of the target sample<br />
relative to the calibrator as log 10 of the 2 −∆∆Ct value. For example, if the bladder has a<br />
log10 relative quantification value of 5 relative to the kidney, then that gene (detector) is<br />
expressed at a level five times higher in the bladder than in the kidney.<br />
Note: Because the calibrator is compared to itself, the expression level for the calibrator<br />
is always 1.<br />
View/Description To view the plot... Example<br />
Gene Expression Plot<br />
Orientation: Detector<br />
Detectors are plotted on the x-axis,<br />
and each bar shows the detector<br />
value of a given sample.<br />
1. In the RQ Results panel,<br />
select the Gene<br />
Expression tab.<br />
2. In the Orientation dropdown<br />
list, select Detector.<br />
Gene Expression Plot<br />
Orientation: Sample<br />
Samples are plotted on the x-axis,<br />
and each bar shows the set of<br />
sample values of a given detector.<br />
1. In the RQ Results panel,<br />
select the Gene<br />
Expression tab.<br />
2. In the Orientation dropdown<br />
list, select Sample.<br />
DRAFT<br />
September 26, 2003 3:14 pm, C5_RQStudy.fm<br />
Notes<br />
38 <strong>Relative</strong> Quantification Getting Started Guide for <strong>7000</strong> v1.1
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