Turmeric Oleoresin

Turmeric Oleoresin
Curcumin
Definition Turmeric Oleoresin consists mainly of curcumin, obtained from the
ground rhizomes of turmeric. Commercial products may contain edible oils.
Color Value The Color Value ( E 10%
1cm ) of Turmeric Oleoresin is not less than 1,500
and is in a range of 90110 % of the labeled value.
Description Turmeric Oleoresin is yellow to dark red-brown powder, lumps,
paste, or liquid having a characteristic odor.
Identification (1) Weigh the equivalent of 0.1 g of Turmeric Oleoresin,
calculated in terms of Color Value 1,500 from the labeled value, dissolve in 200ml of
ethanol. A yellow color with a light-green fluorescence develops.
(2) Turmeric oleoresin solution in ethanol exhibits an absorption maximum at a
wavelength of 420nm430 nm.
(3) Weigh the equivalent of 1 g of Turmeric Oleoresin, calculated in terms of
Color Value 1,500 from the labeled value, dissolve in 100 ml of ethanol, and add
hydrochloric acid until the color of the solution turns slightly orange. Use this solution
as the test solution. Add boric acid to the test solution. A red-orange color develops.
(4) Weigh the equivalent of 1 g of Turmeric Oleoresin, calculated in terms of
Color Value 1,500 from the labeled value, dissolve in 100ml of ethanol, centrifuge the
solution at 3,000 rpm for 10min. Use the supernatant as the test solution. Measure 5 µl
of the test solution and perform Thin-Layer Chromatography without a control solution,
using an isoamylalcoholethanolwaterammonia mixture (4 : 4 : 2 : 1) as the
developing solvent. Two or more yellow spots are observed at Rf 0.40.85. All spots
show yellow fluorescence under UV-light. For the thin-layer plate, use a plate prepared
by applying silica gel for thin-layer chromatography, dried at 110 for 1 hour, as the
support. Stop the development when the solvent front rises about 10 cm above the
original line, air-dry, and observe under daylight and ultraviolet light (wavelength:
about 366nm)
Purity (1) Heavy metals Not more than 40 µg /g as Pb (0.50g, Method 2,
Control solution Lead standard solution 2.0ml)
(2) Lead Not more than 10 µg /g as Pb (1.0g, Method 1)
(3) Arsenic Not more than 4.0 µg /g as AS2O3 (0.50g, Method 3, Apparatus B)

Color Value test Proceed as directed under the Color Value Test.
Operating conditions
Solvent: Ethanol.
Wavelength: Absorption maximum at a wavelength of 420430nm.