Detecting Reporting Resistance in Fastidious Bacteria - SWACM
Detecting Reporting Resistance in Fastidious Bacteria - SWACM Detecting Reporting Resistance in Fastidious Bacteria - SWACM
Recommended Media M45-A2 (Broth microdilution MIC) Plain Mueller Hinton broth – Aeromonas, Plesiomonas, Vibrio, Bacillus, M. catarrhalis, some BT agents Mueller Hinton + 2.5-5% lysed horse blood – same as recommendation for streptococci – Abiotrophia/Granulicatella (+0.001% pyridoxal), Campylobacter, Coryneforms and Corynebacterium spp., Erysipelothrix, HACEK, Lactobacillus, Leuconostoc, Listeria, Pasteurella, Pediococcus (some need CO 2 or other special incubation atmosphere and/or extended incubation) Other – Helicobacter pylori, some BT agents
Can we test M45-A2 organisms with a commercial AST system? Disk diffusion - OK – Number of drug/bugs for which disk diffusion method is described is limited MICs limited – Few tests FDA cleared for M45-A2 organisms; low range of drug concentrations may not be available If NOT FDA cleared – Confirm reliability by performing in house verification / validation – Qualify results Remember: if you cannot confidently test in your laboratory, SEND to a reliable reference laboratory
- Page 1 and 2: Detecting / Reporting Resistance in
- Page 3 and 4: CLSI M45-A2 Guideline Abiotrophia /
- Page 5 and 6: Organisms Included in CLSI M45-A2 M
- Page 7: Table 17. Summary of Testing Condit
- Page 12 and 13: Qualifying Results Optional Comment
- Page 14 and 15: Abiotrophia spp. / Granulicatella s
- Page 16 and 17: CLSI M45-A2 Table 1 Abiotrophia / G
- Page 18 and 19: CLSI M45-A2 Table 1 Abiotrophia / G
- Page 20 and 21: “S” only breakpoint Abiotrophia
- Page 22 and 23: Specimen: Blood Diagnosis: Endocard
- Page 24 and 25: Coryneforms and Corynebacterium spp
- Page 26 and 27: Specimen: Bone marrow Diagnosis: Le
- Page 28 and 29: CLSI M45-A2 Table 13 Pasteurella sp
- Page 30 and 31: Why should we do -lactamase test on
- Page 32 and 33: -Lactamase Testing of M45 Bugs Reco
- Page 34 and 35: Specimen: Cerebrospinal fluid Diagn
- Page 36 and 37: Procedure used in some labs to expe
- Page 38 and 39: HACEK Group Haemophilus aphrophilus
- Page 40 and 41: Specimen: Blood Diagnosis: Endocard
- Page 42 and 43: Moraxella catarrhalis “If desired
- Page 44 and 45: Outcomes of Single Dose Azithromyci
- Page 46 and 47: Table 17. Summary of Testing Condit
- Page 48 and 49: Minimum Laboratory Requirements for
Can we test M45-A2 organisms with a<br />
commercial AST system?<br />
Disk diffusion - OK<br />
– Number of drug/bugs for which disk diffusion method<br />
is described is limited<br />
MICs limited<br />
– Few tests FDA cleared for M45-A2 organisms; low<br />
range of drug concentrations may not be available<br />
If NOT FDA cleared<br />
– Confirm reliability by perform<strong>in</strong>g <strong>in</strong> house verification /<br />
validation<br />
– Qualify results<br />
Remember: if you cannot confidently test <strong>in</strong> your<br />
laboratory, SEND to a reliable reference<br />
laboratory
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