Detecting Reporting Resistance in Fastidious Bacteria - SWACM

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HACEK Group Haemophilus aphrophilus, H. paraphrophilus, H. segnis, Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans, Cardiobacterium spp., Eikenella corrodens, Kingella spp. Often associated with endocarditis – Previous treatment recommendation was ampicillin + gentamicin – Increasing numbers of -lactamase producers – Therapy now often recommend is cefotaxime / ceftriaxone Perform -lactamase test Some Haemophilus spp. and Actinobacillus spp. may be “R” to ampicillin by mechanisms other than -lactamase
MIC Testing of HACEK CAMHB plus 2.5-5% lysed horse blood Some isolates may not grow well using recommended testing conditions Ensure adequate growth in growth control well Sometimes growth enhanced with fresh and maximum amount (5%) of lysed horse blood
Page 1 and 2: Detecting / Reporting Resistance in
Page 3 and 4: CLSI M45-A2 Guideline Abiotrophia /
Page 5 and 6: Organisms Included in CLSI M45-A2 M
Page 7 and 8: Table 17. Summary of Testing Condit
Page 9 and 10: Can we test M45-A2 organisms with a
Page 12 and 13: Qualifying Results Optional Comment
Page 14 and 15: Abiotrophia spp. / Granulicatella s
Page 16 and 17: CLSI M45-A2 Table 1 Abiotrophia / G
Page 18 and 19: CLSI M45-A2 Table 1 Abiotrophia / G
Page 20 and 21: “S” only breakpoint Abiotrophia
Page 22 and 23: Specimen: Blood Diagnosis: Endocard
Page 24 and 25: Coryneforms and Corynebacterium spp
Page 26 and 27: Specimen: Bone marrow Diagnosis: Le
Page 28 and 29: CLSI M45-A2 Table 13 Pasteurella sp
Page 30 and 31: Why should we do -lactamase test on
Page 32 and 33: -Lactamase Testing of M45 Bugs Reco
Page 34 and 35: Specimen: Cerebrospinal fluid Diagn
Page 36 and 37: Procedure used in some labs to expe
Page 40 and 41: Specimen: Blood Diagnosis: Endocard
Page 42 and 43: Moraxella catarrhalis “If desired
Page 44 and 45: Outcomes of Single Dose Azithromyci
Page 46 and 47: Table 17. Summary of Testing Condit
Page 48 and 49: Minimum Laboratory Requirements for

Detecting Reporting Resistance in Fastidious Bacteria - SWACM

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