欧洲鳗鲡外周血细胞的显微和超微结构3

48 (3) :393401 , 2002
A cta Zoologica S inica
3
(
, 130062)
( A nguilla anguilla)
: ;
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:
E2mail : zhouyu69 @sina1com

94 3 48
1 1
( )
Table 1 The size ( long diameter and short diameter) of different blood cells and
classif ied leucocyte counts ( DLC) of one2year2old European eel
Items Erythrocyte Small lymphocyte Large lymphocyte Neutrophil Monocyte Thrombocyte
(10 4 / mm 3 ) 128153 19179
(Number of red blood cell)
( %) 62122 37150 6145 6133 13157 8110 17174 18163
( White blood cell)
(m)
(Long diameter of cell)
(m)
(Short diameter of cell)
(m)
(Long diameter of nuclei)
(m)
(Short diameter of nuclei)
11182 0175 11180 0171 8133 1121 11173 1145 10178 1155 4197 0171
7184 0183 5152 0188 7143 1155 10173 1141 9147 1175 4102 0174
4161 0168 4164 0161 6127 1106 7123 1116 7145 1149 3124 0177
2137 0147 4150 0196 5163 1101 5137 0168 5135 0198 2168 0178
(Data in the table
are all shown as means SD)
(: 9)
, ,
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,
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(: 2 , 3)
21113
21118 ,
21114
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: 1113) ,
21115 ,
,
,
(: 4)
(: 2)
21116
, ,
,
,
1/ 22/ 3 , (: 15)
,
(
,
(: 14)
,
, 212
,
(:
21211
5)
(: 6) ,
21117
5 : , ,
,
,
, , ,
(: 7) (: 16)
,
, ,
, , ,
,
(
,
: 8)
1/ 31/ 2
, ,
,

3 : 395
(: 17)
21212
, , ,
,
, C (: 8)
21213
,
,
,
, 1/ 2 , 311
, 1
(: 18)
21214
,
12
, , ,
,
,
( , 1998) , 1
,
,
,
( , 1985) ( , 1998)
(: 19)
21215
,
( , 1999)
, , Ellis (1977)
,
,
,
,
, (: 20)
Barber (1981)
(: 1)
21216
, ,
,
, , ,
( , 1991 ;
, 1983 ;
, 1985 ; Ellis , 1977 ;
,
(:
Roubal , 1986)
2)
, ( , 1996 ; Yoffey , 1967 ; Parrott et
,
, al . , 1971)
, (: 3)
21217
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,
,
,
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(: 4)
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1
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(: 5 , 6)
(: 7)
3
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, : (Boyar , 1962) (Conroy , 1972)
,
(Mcknight , 1966 ) ( Watson ,
1956) (Watson ,
1962)
, A C Weinreb (1969)

96 3 48
, (
, 1997) ,
, : ( Yuki , 1957)
(Cannon et al . , 1983) ( Ellis , 1976)
,
(Savage , 1983)
Watson (1962)
(Morrow , 1980) ( Ferguson , 1976) Weinreb (1963)
(Savage , 1983) ( Cannon , 1980)
, (1991) 100
,
,
,
1
, Williams (1976)
(: 1) ,
, Cannon (1980)
,
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, Watson
,
(1962) ,
,
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, ( ,
, 1998)
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1999)
( ,
, 1997) , ( Ellis , 1977)
,
,
,
A , B ,
, ( Cannon , 1980) ( Parish
, 1986) (Barber , 1981)
, ,
( , 1998)
,
,
: ,
(Savage , 1983)
Ellis (1977)
,
,
, Barber (1981)
Ferguson
,
(1976)
, , ,
Kusuda (1987)
,
; ,
,
, /
(Cannon , 1980)
314 ,
DLC ,
312 , ( , 1996) ,
,
, ( , 1996) ,
,
,
,

3 : 397
( References)
Barber , D. L . , J . E. M. Westmann and M. G. White 1981 The blood cells of the Antarctic icefish Chaenocephal u acerat us lonnberg : light
and electron microscopic observations. J . Fish Biol . 19 : 1128.
Boyar , H. C. 1962 Blood cell types and differential counts in Atlantic herring , Cl uupea harengus. Copeia. 1 962 : 463465.
Cannon , M. S. , H. Mollenhuer and C. Tompkins 1980 An ulstructural study of the leukocytes of the channel fish , Ictal urus punctat us. J .
Morphology .
164 : 123.
Conroy , D. A 1972 Studies on haematology of the Atlantic salmon ( S al mo salar L . ) . In : Mawdesley , L . and E. Thomas ed. Diseases of
Fish. S ym p. Zool . Soc. L ondon. , No. 30 , London : Academic Press , 101127.
Ellis A E. 1977 The leukocytes of fish : A review. J . Fish Biol . 11 : 453491.
Feng , H. L . W. W. Li and T. H. Wang 1991 Observation on the mocromorphological and super micromorphological structures of the blood
cell of carp. Journal of Fisheries of Chi na. 15 (3) : 241224. [ , , 1991
. 15 (3) : 241244. ]
Ferguson , H. W. 1976 The ultrastructure of plaice Pleuronectes platessa leukocytes. J . Fish Biol . 8 : 139142.
Huang , J . , G. L . Yan and J . P. Jiang 1999 Observation on the microstructrure and ultrastructure of the blood cells of A nguilla anguilla.
Journal of FuJian Teachers U niversity (Natural Science Edition) . 15 (1) : 9094. [ , , 1999
.
(
) 15 (1) : 9094. ]
Kusuda , R. and Yuji Ikeda 1987 Studies on classification of eel leukocytes. Japan. Soc. Sci . Fish 53 (2) : 205210.
Li , Y. N. , Q. Z. Chen and J . Z. Shao 1995 Advance on fish immunology research. Zoological Research 16 : 8394. [ , ,
1995
. 16 : 8394. ]
Lin , G. H. 1996 Ultrastructure of peripheral blood cells of adult grass carp ( Ctenopharyngodon i dell us) . Acta Zoologica Si nica 44 (2) : 123
128. [ 1996
. 42 (2) : 123128. ]
Lin , G. H. and F. W. Zhang 1987 On hematological studies of Xingguo red carp Differential blood count and normal size of blood cells.
Journal of Jiangxi U niversity (Natural Science Edition) 11 (1) : 4148.
. (
) 11 (1) : 4148. ]
[ , 1987
Lin , G. H. , F. W. Zhang and Y. J . Hong 1991 The annual devation of the blood indices of the catfish , Clarias lazera. Acta Zool . Si n.
37 (3) : 341342. [ , , 1991
. 37 (3) : 341342. ]
Lin , G. H. , F. W. Zhang and Y. J . Hong 1998 Hematologenesis in Xingguo red carp. Acta Zool . Si n. 44 (4) : 488489. [ ,
, 1998
. 44 (4) : 488489. ]
Mcknight , I. M. 1966 A haematological study on the mountain whitefish Prosopi um williamsoni . J . Fish. Res. B d. Can. 23 : 4564.
Morrow , W. J . W. and A. Pulsford 1980 Identification of peripheral blood leukocytes of the dogfish Scyliorhi nus canicula L . by electron
microscopy. J . Fish Biol . 17 : 461475.
Mulcahy , M. F. , A. G. Savage and N. Casey 1983 The leukocytes of the pike Esox l uci us L . Advances in fish boilogy in Ireland. Irisheries
Investigations (A) : 24.
Parish , N. 1986 The leukocytes of the elasmobranch Scyliorhi nus canicula L . A morphological study. J . Fish. Biol . 28 : 545561.
Parrott , D. M. V. and M. A. B. Desousa 1971 Thymus2dependent and thymus2independent populations. Origins , migratory patterns and
life span. Cli n. Ex p. I m m unol . 8 : 663684.
Roubal , F. R. 1986 Blood and other possible inflammatory cells in the sparid Acanthopagrus aust ralis ( Gunther) J . Fish Biol . 28 : 573
593.
Savage , A. G. 1983 The ultrastructure of the blood cells of the pike , Esox L uci us L . J . Morphology 178 : 187206.
Wang , Y. Q. Z. Yin and B. Bo 1996 Studies on the blood indices of Misgurnus aruillicaudat us. Acta Sci . N at . U niv . Norm . Hunan.
19 (3) : 7175. [ , , 1996
.
19 (3) : 7175. ]
Watson , L . J . , I. L .
Shechmeister and L . L . Jackson 1962 The haemology of gold fish , Carassi us aurat us. Cyttologia 28 : 118130.
Watson , M. E. , R. W. Guenther and R. D. Royce 1956 Hemotology of healthy and virus diseased sockeye salmon , Onchorhynchus nerka.
Zoologica , N . Y . Zool . Soc. 41 : 2737.
Weinreb , E. L . 1963 Studies on the fine structure of teleost blood cells Iperipheral blood. A nat . Rec. , 147 (2) : 219238.
Williams , R. W. and M. C. Warner 1976 Some observations on the stained cellular elements of channel catfish , Ictalarus punctat us. J .
Fish Biol .
9 : 491497.
Xie , Y. X. and G. H. Lin 1996 Studise on the microstructure and ultrastructure of peripheral blood cell of carassius auratus. Chi nese J . of
Zoology 31 (1) : 1216.
[ , 1996
. 31 (1) : 1216. ]
Xu , H. and Z. Y. Zhang 1983 The fine structure of blood cells in four species of fresh water fishes. Acta Hydrobiologica Si nica 8 (1) : 85
91. [ , 1983
. 8 (1) : 8591. ]

98 3 48
Yan , G. L . J . Huang and D. T. Jiang 1996 Comparative observation on the submicroscopic structures of the blood cells of four species of
vertebrates. Acta A natomica Si nica 27 (1) : 100103. [ , , 1996
. 27 (1) : 100103. ]
Yoffey , J . M. 1967 The fourth circulation. In : Yoffey , J . M. ed. The Lymphocyte in Immunology and Haemopoiesis. London : Edward
Arnold Publ. , 110.
Yuan , S. Q. , Y. A. Zhang and W. J . Yao 1998 Micro and ultrastructure of peripheral blood cells of the mandarin fish , Si niperca chuatsi
(Basilewsky) . Acta Hydrobiologica Si nica 22 (1) : 3945.
.
22 (1) : 3945. ]
Yuki , R. 1957 Blood cell constituents in fish.
3644.
[ , , 1998
. Peroxidase staining of leukocytes in rainbow trout . B ull . Fac. Fish. Hokkai do U niv . 8 :
Zhang , Q. Y. , Z. Q. Li and X. C. Luo 1999 Comparison of the microstructure in the blood cells of the Chinese soft2shelled turtle , frog and
fish. Reservioi r f isheries 19 (3) : 13. [ , , 1999
. 19 (3) : 1
3. ]
Zhu , H. W. , H. Wang and G. Q. Qin 1985 Studies on the blood cell morphoology of crucian carp ( Carassi us aurat us L . ) . Zoological
Research 6 (2) : 147153.
153. ]
[ , , 1985
. 6 (2) : 147
Zhu , Y. X. and G. L . Cao 1997 Studies on the blood cells of eel. Journal of A quacult ure 4 : 1820. [ , 1997
. 4 : 1820. ]
( Abstract)
MICROSTRUCTURE AND ULTRASTRUCTURE OF THE PERIPHERAL
BLOOD CELLS OF EUROPEAN EEL ( AN GU ILL A AN GU ILL A) 3
ZHOU Yu GUO Wen2Chang YAN G Zhen2Guo ZOU Xiao2Huan
ZHAN G Kai WEN Xing2Hao WAN G Tie2Dong
( Faculty of A ni mal Sciences and Veteri nary Medici ne , The Quartermaster U niversity of PL A , Changchun 130062 , Chi na)
We studied the peripheral blood cells of one2year2old European eel ( A nguilla anguilla ) using light
microscopy and transmission electron microscopy. For morphology , peroxidase2Giemsa was used. The
morphological study revealed that the peripheral blood has the following cell types : erythrocyte , neutrophil ,
lymphocyte and thrombocyte. Erythrocytes are 11182 0175m in length and 7184 0183m in width , oval
shape and contain oval nuclei and dark gray cytoplasm. They were almost completely devoid of organelle ,
except for a few vesicles and small mitochondria.
Neutrophils were 11173 1145 m in length and 10173
1114m in width , round or oval , containing round , bilobed or trilobed nuclei and whitish cytoplasm.
were three special granules in neutrophils. Special granules A were round or oval shape , possess thread2like ,
cotton2like or crystalline structrures , special granules B were long oval or rod2like , dark stained and possess
cotton2like structures , which paralleled to the long axia.
gray structures.
There
Special granules C were round or oval , possessed light
The ultrastructural study revealed that the neutrophils include three types of cells according to
their special granules (A , B and C special granules) : type (included A , B and C special granules) type
(included A and C special granules) and type (included C special granules) . A greater amount of
mitochondria , small tubules which resemble smooth endoplasmic reticulum , and numerous oval on elongated
granules which contain a striated or crystalline structure , were observed in neutrophils although neutrophils are
similar to monocytes in size and shape.
Monocytes were of 10178 1155m in length and 9174 1175m in
3 This study was supproted by the Minsitry of Agriculture of China (No. 952B297206201)

3 : 399
width , containing round or oval nuclei. A greater amount of vesicles and a few mitochondria were observed.
Thrombocytes of 1197 0171 m in length and 4102 0174 m in width , were round , oval , spindle or
tadpole2like shape. Deep indentations of the plasmalemma , which gave appearance of vacuoles , were often
seen. Lymphocytes were clearly different from thrombocytes by the presence of large number of free ribosomes.
Neither eosinophils nor basophils were found directly in peripheral blood. The nuclear division of erythrocyte
and monocyte were observed in peripheral blood indicating that erythroblasts and young monocytes could enter
the peripheral blood. The size of thrombocyte was the smallest while that of the neutrophil was the largest and
the number of neutrophils was the least while that of lymphocytes was the most in the blood cells.
Key words European eel ( A nguilla anguilla) , Blood cell , Microstructure , Ultrastructure
( Explanation of Plates)
( Plate
)
1. ( ) ( ) , ( The early stage of growth in the development of erythroblast and small
lymphoidocyte showing pseudopodia) 2 000
2.
( )
( )
( The late stage of growth in the development of erythroblast , the
thrombocyte and the direct nuclear division of red blood cell) 2 000
3.
( The direct nuclear division of red blood cell) 2 000
4.
(Large thrombocyte showing pseudopodia) 2 000
5.
(Monocyte showing cytoplasmic vacuoles) 2 000
6.
( The direct nuclear division of monocyte) 2 000
7. ( Thrombocyte) 2 000
8.
( Tadpole shape thrombocyte) 2 000
9.
( Short stick shape thrombocyte) 2 000
10.
( Spindle shape thrombocyte) 2 000
11.
(Neutrophil showingkidney shapenucleus) 2 000
12.
(Neutrophil showingbell shapenucleus) 2 000
13.
(Neutrophil showing bilobed nuclei) 2 000
14.
(Neutrophil showing round nucleus) 2 000
15.
(Neutrophil showing trilobed nuclei) 2 000
16.
(N) (M) (Np) [ Erythrocyte showing nucleus (N) , cytoplasmic vacuoles (V) and nuclear pore (Np) ]
12 000
17.
(N)
(V) [ Erythrocyte showing nucleus (N) , cytoplasmic vacuoles (V) ] 10 000
18.
(Np)
( P) [ Small lymphocyte showing nuclear pore (Np) and pseudopodia ( P) ] 28 000
19.
( M) ( Nu)
( RER)
( P) [ Large lymphocyte showing mitochondria ( M) ,
nucleolus (Nu) , RER and pseudopodia ( P) ]
16 000
20.
(M) , ( N)
(V) [ Monocyte showing large mitochondria ( M) , nucleolus (N) and cytoplasmic
vacuoles (V) ]
20 000
( Plate
)
1.
(Monocyte showing phagocytose erythrocyte) 11 000
2.
( Thrombocyte showing cytoplasmic vacuoles) 35 000
3.
( Thrombocyte showing cytomembrane ruptured) 7 000
4.
A , B C
, ( M) ( MT) [ type neutrophil showing A , B and C special granules ,
mitochondria (M) , microtube (MT) ]
15 000
5 , 6. AC
, (Rb) [ Showing A , C special granules , free ribosome (Rb) ] 60 000
7.
A , C , (M) (MT) [ type neutrophil showing A , C special granules , mitochondria (M)
and microtube (MT) ]
16 000
8.
C
(N) [ type neutrophil showing C special granules and trilobed nuclei (N) ] 14 000

:
ZHOU Yu et al . : Microstructure and ultrastructure of the peripheral blood cells of European eel
( A nguilla anguilla) Plate

:
ZHOU Yu et al . : Microstructure and ultrastructure of the peripheral blood cells of European eel
( A nguilla anguilla) Plate