PRODUCT CATALOG - Grupo BioMaster

INTERNATIONAL 

PRODUCT CATALOG 

10 /11 

HPLC POST-COLUMN DERIVATIZATION | INSTRUMENTS / COLUMNS / METHODS / REAGENTS

THE CONVENTIONAL WISDOM SAID IT COULDN’T BE DONE. BUT PROFOUND 

NAIVETÉ LED ME TO THINK “HOW COULD I NOT SUCCEED.” WE THEN SET ABOUT 

REVOLUTIONIZING AMINO ACID ANALYSIS. 

Previously you could do amino acid analysis only with a dedicated amino acid analyzer which has almost no flexibility. We developed 

an eluant/column combination and the hardware necessary to transfer the analysis to a modern gradient HPLC. The result was a 

method with the most flexibility that could evolve with the hardware and thus benefit from each generational improvement. Thus 

the user may always have the benefits of the latest HPLC technology. Even today, dedicated amino acid analyzers execute the 

same method as when introduced in the late 50’s.

MICHAEL V. PICKERING, PH.D. / FOUNDER 

As we enter a new decade, I am drawn back to the beginning. After I finished two postdoctoral positions in medicinal 

chemistry, I worked four years in R&D at two different HPLC instrument companies. During that time, I came 

to the realization that the end user does not want capital equipment, solvents, chemicals, or computers. They want 

reliable chromatograms. 

As Aristotle’s theory on causality teaches, the final cause comes first. In this instance, the final chromatogram drives 

the user’s choice for equipment and chemistry. So convinced was I of this truth, I jumped into the void of selfemployment. 

Our product would be the ethereal “Guaranteed Chromatography.” I started Pickering Laboratories 

in 1980, with the patented ninhydrin reagent TRIONE®. Soon thereafter we began offering gradient Amino Acid 

Analysis methods that were achievable with modern HPLC equipment. 

Back then, Amino Acid Analysis run times were 140-minutes for Lithium cation-exchange (native fluid analysis) and 

90-minutes for Sodium cation-exchange (hydrolysate analysis). Today, the same chromatograms are achievable in 

70-minutes for Lithium and 30-minutes for Sodium. 

As you are all aware by now, we work hand in hand with the AOAC, FDA, USDA and USEPA. The relationships 

we’ve cultivated over the years with these regulatory agencies have enabled us to influence analytical methods as they 

develop, and to better understand and support you. In the same context, we are regularly invited to make custom 

formulations and develop pharmaceutical QC methods. 

Our contribution to these regulatory agencies was recently recognized in September of 2009, at the 125th Anniversary 

meeting of the AOAC International in Philadelphia. Pickering Laboratories received AOAC’s Single Laboratory 

Validation of the Year Award for Multiresidue Mycotoxin Analysis in Corn Grain by High-Performance Liquid 

Chromatography with Post-column Photochemical and Chemical Derivatization. Several colleagues and I attended 

the award ceremony – it was an honor for Pickering Laboratories. In fact, the award was doubled to include our 

Single-Laboratory Validation for the Quantification of Neomycin B and Neomycin C in Animal Feeds by Liquid 

Chromatography Fluorescence Detection with Post-column Derivatization. 

Well, here we are, 30 years later. My belief in Aristotle’s theory has turned out to be true. The relationship we have 

created has knit us together as a team: you observe/we support. 

A new range of products is presented in this catalog, so I encourage you to scan it for a solution for your needs. Or, 

call and ask for our advice. 

Your loyalty is our greatest reward. 

01 


PICKERING LABORATORIES 

10/11

TABLE OF CONTENTS 

INTRODUCTION 

3 Customer Service and Technical Support 

3 How to Place an Order 

4 Factory Repairs and Limited Warranty 

5 Instrument Service Plans 

INSTRUMENTS, HARDWARE, 

REACTORS & FLOW CONDITIONERS 

7 Pinnacle PCX Derivatization Instrument 

10 Vector PCX Derivatization Instrument 

12 CRX400 Post-column Reactors 

12 Flow Conditioner 

13 Flow Restrictors 

13 Glyphosate & AMPA Clean-up 

CHROMATOGRAMS, 

COLUMNS & GUARDS 

15 Columns & Guards Catalog Information 

16 Cation-exchange Columns & Guards 

Catalog Information 

17 Reversed-Phase Columns & Guards 

Catalog Information 

17 Specialty Columns 

18 Cation-exchange GARD Column 

Protection System 

18 Lithium Amino Acids 

25 Sodium Amino Acids 

30 Carbamate Pesticide 

34 Glyphosate Herbicide Analysis Column 

35 Alkion Column 

39 Paralytic Shelfi sh Toxins 

40 Mycotox Mycotoxin 

43 Polyether Antibiotics 

44 Analysis of Voglibose in 

Pharmaceutical Formulations 

45 Anion-exchange Column For Nitrite/ 

Nitrate & Bromate Analysis 

46 Analysis of Bromate in Drinking Water 

47 Analysis of Alendronic Acid in 

Pharmaceutical Formulations 

REAGENTS & CHEMICALS 

49 TRIONE Ninhydrin Reagent 

50 o-Phthaladehyde 

50 Naphthalene-2,3-Dicarboxaldehyde 

50 Vanillin 

51 p-Dimethylaminobenzaldehyde (DMAB) 

51 Thiofluor 

51 o-Phthalaldehyde Diluents 

52 Solutions for Sample Preparation for 

Amino Acid Analysis (SERAPREP and 

URIPREP) 

52 Hydrolysis Reagent for Carbamate 

Pesticide Analysis CB130 

52 Hydrolysis Reagent for Carbamate 

Pesticide Analysis CB130.2 

53 Hypochlorite Diluent for Glyphosate 

Herbicide Analysis and Amino Acids 

53 ChlorAC 

54 Restore 

54 Pure Water 

54 Extraction Solution 

54 Samples and Standards Diluents 

(Li220 & Na220) 

55 Calibration Standards and Test Mixtures 

57 Artifi cial Perspiration 

ELUANTS 

58 Sodium-based Solutions for Amino 

Acid Analysis 

58 Lithium-based Solutions for Amino 

Acid Analysis 

58 Glyphosate Herbicide Analysis 

58 Potassium-Based Solutions for 

ALKION Column for Aminoglycoside 

Antibiotics, Paraquat/Diquat in Water 

Analysis & Streptomycin 

58 Biogenic Amines/Polyamines Analysis 

58 Muti-Residue Mycotoxin Analysis 

APPLICATION KITS 

59 30-min High-Effi ciency 

Collagen Hydrolysate 

59 30-min High-Efficiency Protein Hydrolysate 

59 Standard 60-min Protein Hydrolysate 

59 High-Efficiency 55-min Protein Hydrolysate 

59 High-Effi ciency 55-min 


59 60-min Oxidized Feed Hydrolysate 

60 70-min High-Efficiency Physiologic 

Fluid/Native Sample 

60 Standard 185-min Physiologic 


60 High-Effi ciency 120-min Physiologic 


60 Temperature Gradient, Physiologic 

Fluid/Native Sample Kit 

60 High-Effi ciency 7-min PKU 

Detection/Physiologic Fluid 

61 Carbamate Pesticides, 

23+ Compounds 

61 Carbamate Pesticides, EPA 

Method 531.1 

61 Carbamate Pesticides, AOAC 

method 985.23 

61 Carbamate Pesticides, AOAC 

method 531.2 

61 Glyphosate Herbicide, EPA Method 

547, AOAC Method 991.08 

61 Biogenic Amines/Polyamines 

61 Aminoglycoside Antibiotics 

61 Paraquat/Diquat in Water 

61 Multi-Residue Mycotoxin 

61 Afl atoxins 

61 Polyether Antibiotics 

61 Paralytic Shellfi sh Toxins 

BECKMAN 6300/7300 

62 Analytical Columns and Guards 

62 Eluants 

62 Reagents and Chemicals 

REPLACEMENT COMPONENTS, 

FITTINGS & TUBING 

63 Pinnacle PCX Flow Diagram– 

Two-Pump System 

64 Pinnacle PCX Flow Diagram– 

One-Pump System 

65 Vector PCX Flow Diagram 

66 PCX5200 Flow Diagram 

67 Pinnacle PCX Reagent Pump and 

Valve Components 

67 Vector PCX Reagent Pump Components 

67 PCX5200 Reagent Pumps with 

Piston Wash 

67 PCX5200 Reagent Pump Components 

67 Pinnacle PCX Replacement 

Reactor Cartridge 

68 Vector PCX Substitute Reactor 

Volumes or Replacement Reators 

68 PCX5200 Substitute Reactor 

Volumes or Replacement Reactor 

68 Panels for PCX5200 

68 Reagent Reservoirs & Cap Assemblies 

68 Pressure Regulators, Valves & Guages 

69 Flow Restrictors 

69 Filters & Frits 

69 Tees & Unions 

69 Tubings, Plastic 

70 Tubing, Type 316 Stainless Steel 

70 Fittings, Plastic 

70 Fittings, Type 316 Stainless Steel 

71 Fuses & Miscellaneous Accessories 

METHOD ABSTRACTS 

73 Afl atoxins (110) 

74 Multi-Residue Mycotoxin Analysis (203.1) 

76 Multi-Residue Mycotoxin Analysis 

of Dry Grains (208) 

78 Analysis of Alendronic Acid in 

Pharmaceutical Formulations (328) 

79 30-Min Amino Acid Analysis of 

Hydrolyzed Samples (380) 

80 70-Min Amino Acid Analysis of 

Physiological Samples (382) 

81 PKU and MSUD (107) 

82 Aminoglycoside Antibiotics in Feed (205) 

84 Analysis of Biogenic Amines (102)

INTRODUCTION 

86 Bromate Analysis (120) 

88 Post-column LC Analysis of 

23 N-Methyl Carbamates (108) 

90 Expanded HPLC Method for 

N-Methyl Carbamates (112) 

92 Analysis of Formaldehyde (325) 

93 Glyphosate and AMPA Analysis in 

Crops (206) 

95 Simultaneous Determination of Nitrite & 

Nitrate in Processed Foods (123) 

98 Paralytic Shellfi sh Toxins (105.2) 

100 Polyether Antibiotics (104) 

101 Paraquat and Diquat in Water (117) 

102 Sulfonamide Artifi cial Sweetners in 

Food Products (124) 

103 Analysis of Voglibose in Pharmaceutical 

Formulations (327) 

106 General Terms and Conditions of Sale 

107 Distributors 

CUSTOMER SERVICE 

& TECHNICAL SUPPORT 

Pickering Laboratories has earned 

the reputation for strong Technical 

Support in HPLC post-column technology: 

chemistry, columns, reagents, 

and derivatization instruments. We 

start with assisting our customers in 

identifying the best HPLC configuration 

required for a successful integration 

of our HPLC post-column system. 

Then we continue our support 

through and beyond the warranty 

period to ensure our customers’ chromatograms 

reflect current standards 

of quality and reproducibility. 

HOW TO CONTACT US 

For Customer Service or Technical 

Support contact: 

sales@pickeringlabs.com 

support@pickeringlabs.com 

800-654-3330 / 650-694-6700 

Fax: 650-968-0749 

HOW TO PLACE AN ORDER 

Please contact us directly to 

place an order: 

orders@pickeringlabs.com 

800-654-3330 / 650-694-6700 

Fax: 650-968-0749 

Pickering Laboratories, Inc. 

1280 Space Park Way 

Mountain View, CA 94043 

When ordering, please provide 

the following: 

• Complete shipping and billing 

addresses 

• Catalog number and quantity 

of each item 

• Brief product description, as found 

in Ordering Information 

• Purchase order number (and contract 

number, if applicable) 

• Name and telephone number of 

person calling 

• Confirmation required 

• Special instructions, e.g., package markings, 

mode of transit if other than UPS 

• If ordering a complete post-column 

system, please specify the brand 

of HPLC with which it will be 

operating. We will advise you if 

any modifications to the HPLC are 

necessary. 

From European Countries, Please 

Contact our European Distributor: 

LCTech GmbH 

Postfach/P.O. Box 1360 

D-84403 Dorfen, Germany 

Telefon: +49(0)-80 81-93 68-0 

Telefax: +49(0)-80 81-93 68-10 

email: info@lctech.de 

Internet: www.lctech.de 

From non-European countries, please 

contact Pickering Laboratories directly. 

We will provide the names of companies 

which distribute our products. 

TERMS 

• A minimum order is not required 

• Net 30 days for established accounts, 

or prepayment in U.S. dollars, 

C.O.D., or Letter-of-Credit 

• We accept MasterCard & VISA, 

Discover Card or American Express 

• Prices are Ex-Works Seller’s Factory, 

Mountain View, California 

U.S. Destinations & Canada: 

Freight charges are prepaid and 

added to the invoice. Shipments are 

via UPS-Ground unless otherwise 

specified. 

Destinations Outside of the U.S.: 

New Accounts: Before a credit-line 

has been established, new account 

orders must be pre-paid. Freight 

charges are collect. 

Established Accounts in Good Standing: 

Freight charges are collect. Unless otherwise 

specified, shipments overseas 

will be via consolidated air-freight to 

keep the costs as low as possible. 

Prices and specifications are subject 

to change without prior notice. 

03 



10/11

INTRODUCTION 

INSTALLATION & TRAINING 

We encourage you to take advantage 

of Pickering Laboratories’ individualized 

two or three-day installation 

and training courses. For an all-inclusive 

fee we can provide post-column 

system installation and training at 

your site by an experienced chemist 

from Pickering Laboratories or it’s 

representatives. The training includes 

performing analyses, routine maintenance 

and troubleshooting, and 

chromatogram interpretation. 

Some of our authorized distributors 

offer their own training and installation 

services. 

Whenever you buy installation and 

training by Pickering Laboratories, 

please call Technical Support early 

to schedule our visit to your site. This 

is especially important if you buy 

installation through a distributor or 

HPLC Company because we need to 

hear all the details directly from you. 

Individually tailored courses are also 

given at our facility in Mountain 

View, California. Please telephone or 

fax with your requirements and we 

will advise cost and schedule. 

PRODUCT RETURNS 

Products can be returned if unopened. 

A restocking fee of 15 % of 

list price will be applied. Before returning 

a product for any reason, please: 

• Phone or fax requesting a Returned 

Goods Authorization Number. 

• Provide a Purchase Order Number 

for product returns intended for 

service and repair. 

• Package the product so that it will 

arrive undamaged. Use the original 

packing if possible. If in doubt, 

please call. Pickering Laboratories 

cannot assume responsibility for 

goods which arrive damaged in 

shipment. 

• Return the completed Health & 

Safety Declaration with the items 

intended for service or repair (See 

page 4 for details.) 

FACTORY REPAIRS 

Pickering offers factory repair for it’s 

instruments (PCX Model 5200, Pinnacle 

PCX and Vector PCX only). 

We will re-build or replace components 

as necessary in our judgment, 

then test the instrument to make 

certain that the original problem has 

been solved. 

For repairs, we will charge for shipping, 

parts and labor (current shop rate). 

What is Required: 

Obtain a Return Goods Authorization 

Number (RGA) and a Health 

and Safety Declaration form. When 

the instrument is returned it must 

have the RGA noted on the outside 

of the box and a completed Health 

and Safety Declaration, with the 

instrument. To expedite your repair 

we request the instrument is returned 

with a purchase order for the cost to 

evaluate the system (current shop rate). 

What is Done: 

Upon arrival we will evaluate your 

system to generate an estimated cost 

for repairs. Once we have your approval 

we will complete the repair. 

We will replace damaged parts, pressure 

test the instrument, and then 

test the instrument to confirm the 

system meets the products original 

manufacturer’s specification. 

What you get: 

The minimum parts replaced are: 

Restrictor(s), Seal(s) and Filters. If we 

test the instrument to determine that 

it meets the original manufacturer’s 

specification, we will warranty the 

instrument for 1 year on all the parts 

that were replaced. If you request 

that we do not complete this test we 

will not warranty our work. 

FIELD SERVICE 

Because of our limited personnel, we 

prefer to resolve problems without 

site visits, but when that just is not 

enough, we will schedule a site visit 

with you. Please call for a quotation. 

On-site service entails an initial 

evaluation on the first day, with 

repairs potentially extending to the 

second day to allow for shipment of 

parts. The shipment of parts will be 

included in the invoice. 

On-site repairs costs will include 

travel time, air fare, hotel, car rentals, 

expenses, plus parts and labor. 

COLUMN REPACKING SERVICE 

If you have determined or suspect 

that the performance of your 

Pickering cation-exchange column 

or guard has degraded, consider our 

Repacking Service before purchasing 

a new column. Columns eligible for 

repacking include the Pickering amino 

acid, glyphosate and polyamine 

columns and their respective guards. 

If you are not certain that the 

column or guard must be repacked, 

Pickering will test it for a service fee, 

freight or postage prepaid. This fee 

will be credited to any consequent 

repacking cost if tests indicate that 

repacking is needed. 

For this repacking service, Pickering 

will charge 75 % of the current list 

price of that particular column or 

guard. If the column hardware can’t be 

re-used, Pickering Labs will provide a 

new column at 85 % of list price. 

Repacked and tested columns and 

guards carry the same warranty as 

new columns and guards. 

Analytical columns and guards will 

be repacked only one time. 

LIMITED WARRANTY 

Instruments 

Pickering Laboratories, Inc., 

(Pickering) Instruments are warranted 

to be free of defects in material 

and workmanship under normal 

installation, use, and maintenance, 

for a period of one year from the 

date of delivery to the customer. 

Pickering will replace or repair, 

without cost, any defective items. Expendable 

items such as check valves, 

pistons, piston seals, and filters are 

excluded from this warranty. In addition, 

physical damage, poor-quality

INTRODUCTION 

reagent- and sample-induced damage, 

and instrument damage due to 

customer’s misuse are not covered by 

this warranty. 

Analytical Columns 

Pickering’s Analytical Columns are 

warranted for 90 days to be free of 

defects in materials and workmanship 

under normal installation, use, and 

maintenance, for the warranted time 

beginning from the date of delivery 

to the customer. Pickering will replace 

the Analytical Column under warranty 

if found defective in material or 

workmanship. However, the warranty 

is void if the Analytical Column was 

damaged due to customer’s misuse. 

How to Obtain Warranty Service 

If there is a problem with your 

Instrument or Analytical Column 

within the warranty period, immediately 

notify Pickering or your 

local authorized representative. If 

the Instrument or Analytical Column 

was not purchased directly 

from Pickering, please contact the 

vendor where it was purchased. Any 

Instrument, part of the Instrument, 

or Analytical Column returned to 

Pickering for examination or repair 

shall have Pickering’s prior approval 

(call for a Returned Goods Authorization 

Number) and be sent prepaid 

by the Customer. Return transportation 

will be at Pickering’s expense if 

the Instrument, part of the Instrument, 

or Analytical Column is found 

to be defective and under warranty. 

HEALTH & SAFETY DECLARATION 

Instruments and columns must be 

decontaminated and cleaned prior 

to returning to Pickering. To protect 

and ensure the safety of Pickering 

personnel, customer must properly 

flush instrument of any and all hazards 

(e.g. reactive chemicals, blood 

borne pathogens). Please contact 

Pickering to obtain a Health and 

Safety Declaration Form (B-0107) 

describing return instructions and 

customer responsibilities. Failure to 

do so will result in delay of service 

and/or additional charges. 

INSTRUMENT & SERVICE PLANS 

Introducing the Service Plan to 

match the quality and reliability you 

have come to expect from Pickering 

Laboratories. These offerings provide 

factory trained engineers experienced 

in HPLC and post-column 

technologies to service your Pickering 

post-column derivatization instruments. 

Each plan has the features and 

benefits most requested by customers 

to offer the best value and effectiveness 

for today’s busy laboratories. 

When you sign-up for the Pickering 

Laboratories Service Plans you are 

assuring the continued quality and 

reliability of your post-column instrument 

as well as your analysis. 

BENEFITS OF SERVICE AGREEMENTS 

• Free Software Upgrades 

• Installation of all required performance 

upgrades 

• PM/Service performed by Pickering 

Laboratory trained service specialists 

• Fixed Annual Cost – No Surprises 

• Unlimited Technical/Analytical Support 

• Multi-Unit Service Discounts Available 

05 

COMPARISON OF SERVICE PLANS 

FEATURES FACTORY WARRANTY WARRANTY UPGRADE DEPOT SERVICE ELITE SERVICE* 

Telephone support to isolate 

and resolve technical problems 

Monday - Friday 

8AM - 5PM 

Excluding Holidays 


8AM - 5PM 



8AM - 5PM 



8AM - 5PM 


Recertification Not included Included Included Included 

Site visits At prevailing rates Two (including PM) At prevailing rates Two (including PM) 

Preventative Maintenance At prevailing rates One One One 

Freight Charges Not Included Included Included Included 

Parts and Labor Included Included Included Included 

Service Plans offered for the Pickering Laboratories PCX5200, Pinnacle PCX and Vector PCX post-column derivatization instruments. 

Pre-service inspection is required for instruments that have an expired manufacturers warranty or service contracts. 

*The Service plan provides a total of two service calls that will cover parts, labor and travel for the period of one year. Included is unlimited 

telephone support from Pickering Technical Service Engineers and Applications Specialists. 

For more information and quotations, 

please contact Pickering Laboratories’ headquarters Technical Support 

at support@pickeringlabs.com or 800-654-3330. 



10/11

“A LOT HAS CHANGED SINCE PRODUCING OUR FIRST HPLC POST-COLUMN 

DERIVATIZATION SYSTEM, BUT OUR GOALS HAVE REMAINED THE SAME; PROVIDE 

TOP-NOTCH SYSTEMS WITH THE GREATEST FLEXIBILITY AND QUALITY.” 

We have optimized our instruments for post-column applications as well as ease of use, ruggedness and efficiency. 

MICHAEL V. PICKERING, PH.D. / FOUNDER 

SAREETA NERKAR / RESEARCH CHEMIST 

Chalk talk: giving words vision.

INSTRUMENTS & HARDWARE 

PINNACLE PCX DERIVATIZATION INSTRUMENT 

Part of the complete integrated system of instruments, chemicals, columns, methods and support from 

Pickering Laboratories. 

The Pinnacle PCX Sigma Series is an optimized HPLC post-column derivatization system for analysis of Amino Acids, 

Carbamates, Mycotoxins, Antibiotics and many other applications. This instrument is the culmination of Pickering 

Laboratories’ 30 years of experience in post-column analysis instrument manufacturing. Each component is specifically 

designed to optimize the sensitivity and selectivity of post-column analysis. 

Only Pickering Laboratories offers the complete package of chemicals, columns, methods and post-column systems. 

Because each part of the method is designed to work together, Pickering Laboratories can offer the extraordinary 

promise that the analysis is guaranteed to work for the intended application. 

The Pinnacle PCX reflects the ease of use, reliability and ruggedness you have come to expect from 

Pickering Laboratories. 

07 

SYSTEM DESIGN ADVANCEMENTS RESULT IN OPTIMIZED ANALYSIS: 

• The electronic syringe pump provides true pulse-free flow for superior sensitivity and consistency. The pump 

cylinder and head is made from a single piece of inert ceramic for durability and non-reactivity. 

• Electronic valves eliminate troublesome check valves and allow automated pump flushing. 

• The quick-change reactor cartridge makes application switching easy and replacements quick and inexpensive. 

• The column oven utilizes circulating air for consistency of heating and quick cooling. 

• Inert flow path extends system life and reduces maintenance. 

• The PCX control software allows for precise control of the reagent delivery and conservation. 

• Column oven temperature gradient programming improves separation and run times. Pinnacle PCX is the only 

post-column system with this feature. 

• Works with any HPLC system. 

• RoHS compliant 



10/11


FEATURE HIGHLIGHT 

The Pinnacle PCX has a column oven capable of programmable temperature gradients. This feature is useful in Amino 

Acid Analysis by enabling additional flexibility when optimizing methods. Using the temperature gradient program allows 

for improved separation, shortened analysis time and fine-tuned methods for specific compound detection. 

The Chromatogram in Figure 1 demonstrates some of the clear advantages of the temperature gradient program. Compared 

to the standard method without the temperature gradient the total run time is 42 % shorter. Additionally, separation 

is improved. 

With the temperature gradient program, you are able to focus on different areas of the chromatogram for your specific 

compound of interest. 

1 

2 

44 

5 

7 8 11 

10 

9 

20 

23 

15 

17 

16 

o 

18 

70 C 

21 

o 

65 C 

24 

19 

25 

22 

30 

45 

36 

35 

37 

39 

38 

40 

FIGURE 1 

High-efficiency Lithium 

for Physiological samples 

using temperature 

gradient 

26 27 

28 29 

3 

13 

32 

34 

43 

34 oC 

4 

6 

12 

14 

: 

31 

33 

41 

42 

0 10 20 30 40 50 60 70 min 

1 Phosphoserine 

2 Taurine 

3 Phosphoethanolamine 

4 Urea 

5 Aspartic acid 

6 Hydroxyproline 

7 Threonine 

8 Serine 

9 Asparagine 

10 Glutamic acid 

11 Glutamine 

12 Sarcosine 

13 α-Aminoadipic acid 

14 Proline 

15 Glycine 

16 Alanine 

17 Citrulline 

18 α-Amino-n-butyric acid 

19 Valine 

20 Cystine 

21 Methionine 

22 Allo-Isoleucine 

23 Cystathionine 

*Internal Standard 

24 Isoleucine 

25 Leucine 

26 Tyrosine 

27 Phenylalanine 

28 β-Alanine 

29 β-Amino-i-butyric acid 

30 Homocystine 

31 γ-Aminobutyric acid 

32 Tryptophan 

33 Ethanolamine 

34 Ammonia 

35 Hydroxylysines 

36 Ornitine 

37 Lysine 

38 1-Methylhistidine 

39 Histidine 

40 3-Methylhistidine 

41 Anserine 

42 Carnosine 

43 Arginine 

44 Glucosaminic Acid* 

45 2-Aminoethyl-cysteine (AEC)* 

PINNACLE PCX SPECIFICATIONS 

• Dimensions: (h x w x d): 54 x 26.7 x 46.4 cm 

(21.25 x 10.5 x 18.25 inches) 

• Weight: 60 lbs (27.2 Kg) – Duplex 

• Electrical: 100-120 V, 50/60 Hz, 1.7 A, 200 W or 200-240 V, 

50/60 Hz, 0.8 A, 200 W 

• Mains voltage ± 10 % of nominal 

• Installation (over voltage) category II, pollution degree 2 

• Indoor use only 

• Environmental: Altitude up to 6500 ft 

• Ambient Temperature 15 °- 25 °C 

• Relative humidity up to 80 % at 31 °C 

REAGENT PUMPS 

• True pulse-free syringe pump 

• Single piece ceramic barrel 

• Completely inert flow path 

• Maximum operating pressure 500 psi 

• Programmable flow rate


• Flow range: 50 µL to 1500 µL/minute 

• Refill cycle of 60 seconds 

• Automatic piston wash 

• Automatic reagent flush cycle 

• No check valves 

CONTROL 

• Computer controlled 

• Windows PC with 2000, NT, XP 

• USB connection or network 

REACTOR 

• Heated reactor for temperature from 5 °C above ambient 

to 130 °C 

• Easy replacement reactor coil cartridges 

• Range of reactor dwell volumes; 0.1 mL to 3 mL 

• Reaction coil withstands up to 42 bar (600 psi) inlet 

pressure at 130 °C 

• Thermal safety switch limits temperature to 150 °C to 

prevent damage 

• Fast response 

SAFEGUARDS 

• In-line check valve: Prevents reagent back flow into the column 

when HPLC pressure drops 

• Post-column system over pressure: A pre-calibrated relief valve 

opens at 35 bar (500 psi) to prevent rupture of the post-column 

reactor tubing in the event of down-stream blockage 

• Back-pressure regulator: Applies 7 bar (100 psi) to the detector 

flow cell outlet (waste) to prevent detector noise and 

precipitation due to out-gassing or boiling 

COLUMN HEATER AND REACTOR CONTROLLER 

• Heater accepts up to 6 or 8 mm OD 

(0.25 or 0.31 inch) x 50-250 mm in length (column and guard) 

• Programmable temperature gradient 

• Power cooling capability 

• Temperature holds within ± 0.4 °C from the set point. Can be set 

with 1 °C resolution from 5 °C above ambient to 75 °C 

• Easy column access 

INSTRUMENT PACKAGE AND FLOW PATH 

• Advanced fluidics valve management system 

• Easy access to internal components 

• Standard fittings 

• Post-column pressure relief valve 

• Side panels easily removed for service access 

• Integrated reagent reservoir tray 

• Corrosion proof pan and panels 

DISPLAY 

• Backlit LCD 

• Real time temperature and pressure display 

• System status icons 

• Simple system control interface 

GAS PRESSURE MANIFOLD AND REGULATOR 

• Panel mount manifold 

• Regulator maintains 0.3 bar (3-5 psi) on reagent 

reservoirs with 3-5 bar (45-75 psi) source pressure 

• Safety pressure relief valve opens at 1 bar (14 psi) 

• Manifold has two 1/4-28 tubing connections 

• Gas lines with anti-syphon valves 

PRESSURIZED REAGENT RESERVOIR 

• One liter capacity (2 and 5 L reservoirs available) 

• Maintained under inert gas pressure to inhibit 

oxidation of oxygen sensitive reagents 

• Valve built into reservoir cap permits sparging 

during reagent preparation 

• Reagent reservoirs fitted with 3.1 mm (1/8”) 

OD, oxygen-impermeable SARAN tubing for 

oxygen-sensitive reagents 

09 

PINNACLE PCX CATALOG INFORMATION 

CATALOG NO. APPLICATION REAGENT PUMP REACTOR VOLUME VOLTAGE 

1153-1011 

1153-1012 

1153-1021 

1153-1022 

1153-1031 

1153-1032 

1153-1041 

1153-1042 

1153-1051 

1153-1052 

1153-1091 

1153-1092 

1153-1061 

1153-1062 

1153-1071 

1153-1072 

1153-1081 

1153-1082 

1153-1101 

1153-1102 

Amino Acids (OPA detection), Aminoglycoside Antibiotics, Biogenic Amines, 

Polyamines, Paraquat/Diquat 

Amino Acids (TRIONE® Ninhydrin detection), Ochratoxin A, Formaldehyde, 

Bromate 

Single 0.15 mL 120 V 

240 V 

Single 0.5 mL 120 V 

240 V 

Mycotoxins including Aflatoxins & Fumonisins Single Knitted 

1.4 mL 

Cholesterol Single Knitted 

2.8 mL 

120 V 

240 V 

120 V 

240 V 

Carbamate Pesticides, Glyphosate Herbicide, Streptomycin, (OPA detection) Dual 0.5 mL 120 V 

240 V 

Micro System for Carbamate Pesticides & Glyphosate Herbicide Dual 0.1 mL 120 V 

240 V 

Paralytic Shellfish Toxins Dual Knitted 

1.0 mL 

Trichothecene Mycotoxins Dual Knitted 

1.2 & 1.6 mL 

Polyether Antibiotics Dual Knitted 

1.4 mL 

Voglibose Analysis Single Knitted 

3.5 mL 

NOTE: Columns and chemicals are sold separately 

120 V 

240 V 

120 V 

240 V 

120 V 

240 V 

120 V 

240 V 



10/11


• Indoor use only 

• Environmental: altitude up to 6,500 ft 

• Ambient temperature 15 º - 25 ºC 

• Relative humidity up to 80 % at 31 ºC 

VECTOR PCX DERIVATIZATION INSTRUMENT 

The Vector PCX instrument is the newest addition to the 

Pickering family of post-column derivatization systems. 

Introduced a year after the Pinnacle PCX launch, the new 

Vector PCX serves as another post-column choice ideal for 

application-specific methods. 

Vector PCX provides the selectivity and sensitivity required 

for most standard post-column applications while being 

reliable and easy to use. Since Vector PCX does not have a 

column oven it is important to use the HPLC column oven 

to ensure stable column temperature and prevent retention 

time drifts and separation problems. 

Designed as a step-up from our previous model PCX5200, 

the Vector PCX has several improvements and innovations. 

VECTOR PCX FEATURES 

• Inert pumps 

• PEEK liquid manifolds 

• Integrated gas manifold 

• Easy access and monitoring of fluidics 

• Simple control interface (no computer required) 

• Reduced bench space profile 

Matched with Pickering’s Chromatographic Grade ® reagents, 

eluants and columns, the Vector PCX instrument is a solid 

choice for laboratories’ standard post-column needs. 

VECTOR PCX SPECIFICATIONS 

• Dimensions: (h x w x d): 43 x 21.6 x 41.2 cm 

(17 x 8.75 x 16 inches) 

• Weight: 11.5 kg (25.3 lb.) 

• Electrical: 100 - 120 V, 50/60 Hz 1.7 A, 200 W or 

200-240 V, 50/60 Hz, 0.8 A, 200 W 

• Mains voltage ± 10 % of nominal 

• Installation (over voltage) category II, pollution degree 2 

REAGENT PUMPS 

• Independently adjustable, low-pulsation 

• Adjustable from 0.05 to 2.00 mL/minute against back-pressures 

of up to 2000 psi 

• Flow Accuracy 3 % for flowrates of 0.33 ml/min and above, 0.01 

ml/min for flowrates below 0.33 ml/min 

• Flow Precision 0.5 % RSD 

• Sapphire pistons 

• Liquid ends, including check valve housing, PEEK 

• PEEK bypass/purge valves for each pump located on front of 

instrument panel 

• Automatic piston wash 

FLOW PATH 

• Independent pressure transducer for each pump 210 bar 

(0-3000 psi) 

• Diamond-packed restrictors, matched to flow rate and viscosity 

of reagents 

• PEEK bypass/purge valves 

• Replaceable reagent filter 

• PEEK mixing manifold 

REACTOR 

• Heater reactor controls at ±0.4 ˚C for temperatures from 5 ˚C 

above ambient to 130 ˚C 

• Range of reactor dwell volumes, 0.1 mL to 3 mL 

• Reaction coil withstands up to 42 bar (600 psi) inlet pressure 

at 130 ˚C 

• LCD display of actual temperature or set point 

• Thermal safety switch limits temperature to 150 ˚C 

GAS PRESSURE MANIFOLD AND REGULATOR 

• Panel mounted manifold 

• Regulator maintains 0.3 bar (3-5 psi) on reagent reservoirs with 

3-5 bar (45-75 psi) source pressure 

• Safety pressure relief valve opens at 1 bar (14 psi) 

• Manifold has two 1/4-28 tubing connections 

• Gas line with anti-siphon valve 

PRESSURIZED REAGENT RESERVOIR 

• One liter capacity (2 and 5 L reservoirs available) 

• Maintained under inert gas pressure to inhibit oxidation of oxygensensitive 

reagents 

• Valve built into reservoir cap permits sparging during reagent 

preparation 

• Reagent reservoirs fitted with 3.1 mm (1/8’’) OD, oxygen-impermeable 

SARAN tubing for oxygen-sensitive reagents and/or with 

3.1 mm (1/8’’) OD FEP tubing.


SAFEGUARDS 

• A pressure switch installed between LC (eluant) pump and sample 

injector turns off power to reagent pumps and reactor when the 

eluant pump pressure drops to 35 bar (500 psi), ensuring that 

reagent will not flow upstream and damage the analytical column. 

Low eluant pressure can result from power failure, eluant pump 

malfunciton, automatic or intentional shut-down, or an empty 

reservoir. The Vector PCX will not restart automatically. 

• Post-column system over pressure: A pre-calibrated relief valve 

opens at 35 bar (500 psi) to prevent rupture of the post-column 

reactor tubing in the event of down-stream blockage 

• Back-pressure regulator: Applies 7 bar (100 psi) to the detector 

flow cell outlet (waste) to prevent detector noise and precipitation 

due to out-gassing or boiling 

VECTOR PCX CATALOG INFORMATION 

CATALOG NO. APPLICATION REAGENT PUMP REACTOR VOLUME VOLTAGE 

1154-4051 

1154-4052 

1154-4001 

1154-4002 

1154-4061 

1154-4062 

1154-4071 

1154-4072 

1154-4081 

1154-4082 

1154-4101 

1154-4102 

Carbamate Pesticides, Glyphosate Herbicide, Streptomycin, (OPA detection) Dual 0.5 mL 120 V 

240 V 

Streptomycin Dual 0.5 mL 120 V 

240 V 

Paralytic Shellfish Toxins Dual Knitted 

1.0 mL 

Trichothecene Mycotoxins Dual Knitted 

1.2 mL and 1.6 mL 

Polyether Antibiotics 

Inert PEEK 

Dual 

Knitted 

1.4 mL 

Voglibose Analysis Single Knitted 

3.5 mL 

NOTE: Please contact Pickering for assistance in confi guring a custom Vector PCX that will meet your specifi c analysis needs. 

All confi gurations include gas manifold, accessories, spares, reservoir(s), power cord and operation manual. 

120 V 

240 V 

120 V 

240 V 

120 V 

240 V 

120 V 

240 V 

11 

CATALOG NO. 

CONNECTION TUBING KITS 

DESCRIPTION 

1100-0450 Tubing Kit to connect Vector PCX & Pinnacle PCX to HPLC 

1100-0460 Dead-head Plumbing Kit for HPLC 

NOTE: Dead-head circuits reduce the possibility of corrosion and minimize unswept volume, resulting in 

more precise gradient control. Dead-head kits include tee, plug, tubes, fittings. 



10/11


CRX400 POST-COLUMN REACTORS 

CRX400 Post-column Reactor is designed for use with 

standard liquid chromatography equipment. 

Available standard reaction coil volumes are listed below. 

If these volumes are not satisfactory, please contact us with 

your specific requirements. 

Replacements or substitution of a reaction coil is easily 

accomplished in the laboratory. Reactor Heater and Coil 

Assemblies are insulated, self-contained units, comprising 

the reactor coil with inlet and outlet tubing and fittings, and 

heater with electrical connection. 

To prevent reactor damage we suggest the installation of a 

pressure relief valve assembly (1100-0710) 

CRX400 SPECIFICATIONS 

• Operating temperature starting from 5 °C above ambient to 130 ºC 

• Set temperature is maintained at ± 0.4 °C within control range 

• 120 or 240-Volt configurations, 50/60 Hz 

• Dimensions: (cm) 12 W X 14 H X 28.5 L 

• Shipping weight : 2 kg (4.4 lbs) 

PULSE-DAMPENER: 

FC4870B FLOW CONDITIONER 

The Pulse-Dampener is intended for use in Liquid Chromatography 

applications where continuous post-column 

derivitization is desired. Installed at the outlet of the pump, 

the model FC4870B Flow Conditioner provides the pulsedampening 

necessary to maintain a smooth baseline. Also 

the following important features are provided: 

• Continuous filtering of the pumped medium 

• Working pressure for the reagent pump: check values perform 

more reliably which results in a more stable flow 

• Pressure reading (gauge) for the reagent pump 

• Prime/purge valve 

The gauge and pressure relief valve assembly consists of a 

cross for connecting a pressure gauge, an anti-siphon valve 

and a pre-calibrated relief valve. This is used to indicate 

post-column pressure and to prevent rupture of the postcolumn 

reactor tubing in the event of an overpressure 

condition. The anti-siphon valve prevents the reservoir 

gas pressure from pumping reagent while the system is 

turned off. 

PULSE-DAMPENER/FLOW CONDITIONER CATALOG INFORMATION 


1100-0093 

DESCRIPTION 

FC4870B Flow Conditioner, with pressure gauge & prime/purge 

valve, for OPA (with 1100-0200 restrictor) 

1100-0094 

FC4870B Flow Conditioner, with pressure gauge & prime/purge 

valve, for TRIONE® (with 1100-0141 restrictor) 

1100-0710 Post-column Gauge and Pressure Relief Valve Assembly 

CRX400 CATALOG INFORMATION 

CATALOG NO. APPLICATION Max. 

Temp. 

1100-3329 

1100-3330 

1100-3331 

1100-3332 

1100-3341 

1100-3342 

1100-3323 

1100-3324 

1100-3351 

1100-3352 

1100-3343 

1100-3344 

1100-3325 

1100-3326 

1100-3321 

1100-3322 

CRX400 Post-column Reactor, 0.15 mL, 120 V 






CRX400 Post-column Reactor, 1.2 mL and 1.6 mL, 120 V 

CRX400 Post-column Reactor, 1.2 mL and 1.6 mL, 240 V 









130 ºC 

130 ºC 

130 ºC 

130 ºC 

130 ºC 

130 ºC 

75 ºC 

75 ºC


SAMPLE CLEAN-UP 

FLOW RESTRICTORS 

Pickering restrictors are tubes packed with sized diamond 

particles designed to provide a desired back-pressure, relative 

to a given flow rate and viscosity. The restrictor tube 

and fittings are type 316 stainless steel or PEEK. The diamond 

packing is chemically and chromatographically inert 

to all mobile phases. 

FEATURES 

• Pressure-calibrated to flow rate and viscosity 

• Diamond-packed tubes with nuts and ferrules 

• Improve pump efficiency 

• Essential pulse-dampening component 

• Troubleshooting aid for HPLC systems 


FLOW RESTRICTORS CATALOG INFORMATION 

DESCRIPTION 

1100-0141 Flow restrictor, TRIONE® Ninhydrin reagent, 65 psi, 6 cm 

1100-0200 Flow restrictor, OPA, Hypochlorite, or Hydrolysis reagent, 300 psi, 10 cm 

1100-0262 Flow restrictor, 1150 psi, 20 cm 




1100-0263 Flow restrictor, non-metallic (PEEK), 550 psi, 10 cm 

NOTE: Listed pressures are for a fl ow rate of 0.3 mL/min at ambient temperature 

with 100 % water and pulse-dampened fl ow. 

GLYPHOSATE AND AMPA SAMPLE 

CLEAN-UP CARTRIDGES 

Pickering SPE sample clean-up cartridges are used to treat 

samples prior to injection. The strong cation-exchange resin 

is composed of sulfonic acid functional groups attached 

to a styrene divinylbenzene copolymer lattice. The high 

selectivity and ruggedness provides a simple and universal 

method for removing matrix interference from the sample. 

The SPE sample clean-up cartridges are ideal for the cleanup 

of vegetables, fruits and crop samples in the analysis of 

Glyphosate and AMPA. 

RECOVERIES FOR GLYPHOSATE AND AMPA 

MATRIX GLYPHOSATE AMPA 

Alfalfa 129 % 116 % 

Strawberry 84 % 82 % 

Broccoli 97 % 95 % 

Sample Preparation 

Extraction: 

Take 25 g of homogenous sample and add enough water 

(after estimation of moisture content) to make the total 

volume of water 125 mL. Blend and Centrifuge. 

Matrix Specifi c Modifi cation: 

• High water content – reduce sample amount to 12.5 g 

• High protein content – add 100 μL HCl to 20 mL of 

extract, shake and centrifuge. 

• High Fat content – perform the methylene chloride 

partition twice. 

Clean-up 

Methylene Chloride Partition: 

• To 20 mL of aqueous extract add 15 mL methylene 

chloride. Shake for 2-3 min and centrifuge. 

• To 4.5 mL of aqueous layer add 0.5 mL acidic modifier 

solution. Shake and centrifuge. 

SPE Clean-up: 

• Prepare SPE cartridge (refer to product abstract PA210) 

• Transfer 1 mL portions of SPE mobile phase (refer to 

method abstract MA206 for formulation) and discard 

the effluent. 

• Elute analytes with 12 mL SPE mobile phase 

Concentration: 

• Evaporate to dryness using rotary evaporator or a vacuum 

vortex-type evaporator or lyophilize 

• Re-dissolve in 2 mL of the SPE mobile phase 

13 

CATALOG NO. DESCRIPTION QTY 

1705-0001 SPE sample clean-up cartridges 50/pkg 



10/11

“WE DON’T DESIGN OUR COLUMNS TO HAVE MILLIONS OF THEORETICAL PLATES.” 

The column is the key component of each unique assay. Stationary phases are chosen for their chemical selectivity; each 

column is optimized for a single application. Each column is tested with the analyte sets that comprise the method and the 

QC chromatogram is included with the column. After all, that’s what the user wants. 

MARIA OFITSEROVA / SENIOR RESEARCH CHEMIST SAJI GEORGE / QC MANAGER WENDY RASMUSSEN / TECHNICAL SALES REP. 

Hardware, Tubes, Chemistry and 

thoughts are gathered.

CHROMATOGRAMS, COLUMNS & GUARDS 

Pickering Laboratories columns and guards are intended for specific applications that require post-column derivatization. 

This technology guarantees detection of certain classes of compounds at very low concentrations—amino acids, carbamate 

pesticides and polyamines, for example. 

Each column is packed and tested to separate the target compounds according to Pickering’s chromatographic quality 

control standards and published analytical method. The following acceptance criteria apply to all of Pickering’s columns: 

• With guard column installed, produce a specified chromatogram 

of the compounds in a standard test mixture. 

• Separate the compounds in the established order, with specified resolution of critical pairs. 

• Operate within the specified range of back pressure. 

• Be free of contaminating material which can cause baseline artifacts. 

Only after all criteria have been met can the column’s serial number and label be applied. 

Quite simply, the column is guaranteed to produce a chromatogram for its intended application if it is operated according 

to the conditions and methods prescribed by Pickering Laboratories. 

About Guard Columns 

While it is true that any of our columns may be run without a guard, the 

practical consequence is a shorter column lifetime. Guard columns provide 

protection against contamination without affecting column efficiency. It is 

far less expensive to replace or repack a guard than an analytical column. 

15 



10/11


CATION-EXCHANGE COLUMNS & GUARDS CATALOG INFORMATION 


DESCRIPTION 

LITHIUM AMINO ACID ANALYSIS COLUMNS 

0354100T 

0354675T 

High-efficiency Lithium Cation-exchange Column 4.0 x 100 mm &1700-0070 amino acid test mixture 

70 min High-efficiency Lithium Ion-exchange Column, 4.6 x 75 mm 

0393250 Standard Lithium Cation-exchange Column 3.0 x 250 mm &1700-0070 amino acid test mixture 

0354050 High-efficiency Cation-exchange Column for PKU and MSUD screens 4.0 x 50 mm 

0352020 Lithium Guard Column, 2.0 x 20 mm (for use with 0354100T) 

0392020 Lithium Guard Column, 2.0 x 20 mm (for use with 0393250 column) 

1154150 

1154150T 

1154110T 

SODIUM AMINO ACID ANALYSIS COLUMNS 

High-efficiency Sodium Cation-exchange for protein collagen hydrolysates Column 4.0 × 150 mm & 1700-0070 

amino acid test mixture 

High-efficiency Sodium Cation-exchange for protein, collagen and oxidized feed hydrolysates Column 4.0 × 150 mm & 

1700-0070 amino acid test mixture 

30-minute High-efficiency Sodium Cation-exchange Column 4.6 × 110 mm & 1700-0070 amino acid test mixture 

1193250 Standard Sodium Cation-exchange Column 3.0 × 250 mm & 1700-0070 amino acid test mixture 

1193020 Sodium Guard Column, 3.0 × 20 mm (for use with 1154150 and 1154150T Columns) 

1192020 Sodium Guard Column, 2.0 × 20 mm (for use with 1193250 Column) 

GLYPHOSATE COLUMNS 

1954150 Cation-exchange Column for Glyphosate analysis, 4 × 150 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL 

1953020 Cation-exchange Guard Column, 3.0 × 20 mm 

1952150 Micro Cation-exchange Column, K+ form, 2.1 × 150 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL 

1954250 Column, Glyphosate 4 x 250 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL 

ALKION COLUMN 

9410917 ALKION Cation-exchange column, K form, 4.0 × 150 mm 

9493020 ALKION Guard column, K form, 3.0 × 20 mm 

GARD COLUMN PROTECTION SYSTEM (FOR USE WITH ANY CATION-EXCHANGE COLUMN) 

1700-3102 Cation-exchange GARD Assembly: Includes holder and 2 replaceable GARDS 

1700-3101 Replacement Cation-exchange GARDs (2/PK) 

1700-3100 GARD Holder


REVERSED-PHASE COLUMNS & GUARDS CATALOG INFORMATION 


DESCRIPTION 

CARBAMATE PESTICIDE COLUMNS 

0840250 Carbamate Column, C 8 

, expanded resolution, 4.0 × 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL 


, high resolution/capacity, 4.6 × 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL 


, rapid analysis, 4.6 × 150 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL 

POLYETHER ANTIBIOTICS ANALYSIS 

2381750 Polyether reversed-phase column, C 18 

, 4.6 × 250 mm 

PARALYTIC SHELLFISH TOXINS ANALYSIS 

0846150 Reversed-phase column for analysis of paralytic shellfish toxins, C 8 

, 4.6 × 150 mm 

MYCOTOXINS ANALYSIS 

1612124 MYCOTOX Reversed-phase column, C 18 

, 4.6 × 250 mm 

GUARDS FOR STANDARD REVERSED-PHASE COLUMNS 

18ECG001 

18ECG002 

Guard Cartridge Holder with 3 cartridges 

Guard Cartridges, 2/pk 

GUARDS FOR MICRO REVERSED-PHASE COLUMNS 

1700-0204 Guard Cartridge Holder with 3 guard cartridges, for use with Micro columns 

1700-0205 Guard Cartridges, 2/pk, for use with Micro columns 

SPECIALTY COLUMNS 


DESCRIPTION 

0785150 Anion-exchange Column for Bromate and Nitrite/Nitrate Analysis (4.6 × 150 mm), 5 µm 

17 

1446250 Amino Column for Voglibose Analysis (4.6 × 250 mm), 5 µm 



10/11


CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM 

The new GARD uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting the 

analytical column), but allows the analytes of interest to pass unimpeded through the GARD and onto the analytical column. 

The new GARD significantly prolongs column life without band spreading or added pressure. We demonstrated, by 

means of a performance comparison for Amino Acid Analysis, that the use of a GARD will effectively protect the analytical 

column, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications, 

and most importantly has zero band spreading. 

Wth the new GARD Column protection system from Pickering Laboratories the same cation-exchange GARD can be 

used for nearly all cation-exchange applications. 

LITHIUM AMINO ACID ANALYSIS COLUMNS 

Pickering Laboratories specialize in manufacturing of cation-exchange columns for amino acid analysis. No other techniques, 

including reversed-phase chromatography, have been shown to match post-column ion-exchange methods in quantitation 

and reproducibility. Advantages of this method, such as absence of matrix interferences, are especially important in 

analysis of native samples. 

Lithium columns and buffers systems have high selectivity and are perfect for physiological fluids and food analysis. 

Post-column Conditions For Amino Acids Analysis: 

Reagent: Trione® 

Reactor: 130 °C, 0.5 mL 

Reagent Flow Rate: 0.3 mL/min 

Detection: 

UV-Vis Detector: λ=570 nm for primary amino acids 

λ=440 nm for secondary amino acids 

or 

Reagent: 300 mg of OPA, 2 g Thiofluor, 3 mL of 30 % 

Brij® 35 solution in 950 mL of OD104 



Detection: 

Fluorometer: λ ex 

330 nm, λ em 

465 nm 

The recommended gradient conditions are subject to 

change without notice. This may happen because of lot 

changes in the columns, or improvements in the overall 

method. 

The recommended gradient for the column will always 

be included in the column package, and it supersedes the 

information in this catalog. 

Pickering Laboratories recommends six different gradient 

conditions depending on the column and type of sample. 

Use the program recommended on the column data sheet 

for the initial testing. 

The column oven temperature programming gives additional 

flexibility when optimizing methods. Using temperature 

gradient allows to improve separation, shorten analysis time 

and fine-tune the method for detecting compounds 

of interest.


70-MIN HIGH-EFFICIENCY LITHIUM CATION-EXCHANGE COLUMN (4.6 X 75 mm) 

CATALOG NUMBER 0354675T 

USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM 

USE FOR PHYSIOLOGICAL SAMPLES 

TEMPERATURE GRADIENT 

HPLC PROGRAM 

TIME 1700-1125 % Li365 % Li375 % RG003 % 

0 100 0 0 0 

10 100 0 0 0 

19 40 60 0 0 

32 0 100 0 0 

43 0 100 0 0 

43.1 0 0 100 0 

57 0 0 100 0 

57.1 0 0 70 30 

72 0 0 70 30 

72.1 100 0 0 0 

HPLC FLOW RATE: 0.55 mL/min, INITIAL TEMP.: 34 ºC 

COLUMN OVEN PROGRAM 

TIME TEMP ºC 

0 34 

6 34 

17 65 

25 70 

70 70 

71 34 

REAGENT FLOW RATE: 0.5 mL/min 

1 

2 

44 

5 

7 8 11 

10 

9 

20 

23 

15 

17 

16 

18 21 

24 

19 

25 

22 

30 

45 

36 

35 

37 

39 

38 

40 

19 

26 27 

28 29 

3 

13 

32 

34 

43 

4 

6 

12 

14 

: 

31 

33 

41 42 

0 10 20 30 40 50 60 70 min 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

12 

Phosphoserine 

Taurine 

Phosphoethanolamine 

Urea 

Aspartic acid 

Hydroxyproline 

Threonine 

Serine 

Asparagine 

Glutamic acid 

Glutamine 

Sarcosine 


14 Proline 

15 Glycine 

16 Alanine 

17 Citrulline 


19 Valine 

20 Cystine 

21 Methionine 

22 Allo-Isoleucine 


24 Isoleucine 

25 Leucine 

26 Tyrosine 


28 β-Alanine 




32 Tryptophan 


34 Ammonia 


36 Ornitine 

37 

38 

39 

40 

41 

42 

43 

44 

45 

Lysine 

1-Methylhistidine 

Histidine 


Anserine 

Carnosine 

Arginine 

Glucosaminic Acid* 

2-Aminoethyl-cysteine (AEC)* 


NOTE: This method utilizes column temperature gradient. Use Pinnacle PCX 

column oven or HPLC column oven with temperature gradient capabilities. 



10/11


HIGH-EFFICIENCY LITHIUM CATION-EXCHANGE COLUMN (4.0 X 100 mm) 


USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR LITHIUM GUARD COLUMN 035202 (2.0 X 20 mm) 

USE FOR PHYSIOLOGICAL SAMPLES AND COMPLEX MATRICES 

TEMPERATURE ISOCRATIC 

1 2 

44 

3 

5 

17 

15 16 20 

18 

22 

7 8 9 10 11 12 

13 

4 

6 

14 

19 

21 

23 

24 25 

26 27 

30 

29 

28 

31 

32 

33 34 35 

36 

37 

38 

39 

40 

41 

42 

43 

45 

0 25 50 

75 100 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

12 

Phosphoserine 

Taurine 


Urea 

Aspartic acid 


Threonine 

Serine 

Asparagine 

Glutamic acid 

Glutamine 

Sarcosine 

13 α−Aminoadipic acid 

14 Proline 

15 Glycine 

16 Alanine 

17 Citrulline 

18 α−Amino-n-butyric acid 

19 Valine 

20 Cystine 

21 Methionine 


23 Isoleucine 

24 Leucine 

25 Norleucine 

26 Tyrosine 


28 β−Alanine 

29 β−Amino-i-butyric acid 


31 γ−Aminobutyric acid 

32 Tryptophan 



35 Ammonia 

36 Creatinine 

37 Ornithine 

38 Lysine 

39 Histidine 

40 3-Methyl-histidine 


42 Carnosine 

43 Anserine 

44 α−Amino−β− 

guanidinopropionic acid 

45 Arginine 

CONDITIONS 

STEP TIME (Min) INTERVAL Li275 % Li750 % RG003 % COMMENT 

0 0 0 100 0 0 Inject 

1 8 8 100 0 0 Isocratic 

2 46 38 65 35 0 Linear Gradient 


4 90 4 0 100 0 Isocratic 


6 122 2 0 94 6 Isocratic 

7 122.1 0.1 100 0 0 Step Change 

8 140 17.9 100 0 0 Re-equilibration 

FLOW RATE: 0.35 mL/min, COLUMN TEMP: 37 ºC, 

INJECTION VOLUME: 10 µL of 0.25 µmole/mL Std.





USE FOR PHYSIOLOGICAL FLUIDS 


CONDITIONS 

STEP TIME (Min) 1700-1125 % Li365 % Li375 % RG003 % COMMENT 

0 0 100 0 0 0 Inject 

1 15 100 0 0 0 Isocratic 



4 60 0 100 0 0 Isocratic 

5 60.1 0 0 100 0 Step Gradient 

6 78 0 0 100 0 Isocratic 

7 78.1 0 0 70 30 Step Gradient 

8 95 0 0 70 30 Isocratic 

9 95.1 100 0 0 0 Step Gradient 

10 115 100 0 0 0 Equilibration 



TIME (Min) TEMPERATURE ºC 

0 34 

13 34 

30 65 

67 66 

80 70 

97 70 

98 34 

1 

2 

20 

16 

15 17 

18 

22 

21 

5 

8 

7 

19 

21 

35 

36 

3 43 

10 

23 24 

25 

26 

29 

34 

37 38 

39 

4 

6 

9 

13 

11 

12 

14 

27 28 

30 

32 

31 

33 

40 41 42 

0 20 40 60 

80 min 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

Phosphoserine 

Taurine 


Urea 

Aspartic acid 


Threonine 

Serine 

Asparagine 

Glutamic acid 

Glutamine 

12 Sarcosine 


14 Proline 

15 Glycine 

16 Alanine 

17 Citrulline 


19 Valine 

20 Cystine 

21 Methionine 


23 Isoleucine 

24 Leucine 

25 Tyrosine 


27 β-Alanine 




31 Tryptophan 


33 Ammonia 

34 

35 

36 

37 

38 

39 

40 

41 

42 

43 

Hydroxylysines 

Ornitine 

Lysine 


Histidine 


Anserine 

Carnosine 

Arginine 




column oven or HPLC column oven with temperature gradient capabilities. 



10/11

Valine 

Norleucine 





USE FOR PKU/MSUD SCREENING OF PHYSIOLOGICAL FLUIDS 


CONDITIONS 

STEP TIME (Min) INTERVAL Li275 % Li750 % RG003 % COMMENT 

0 0 0 86 14 0 Inject 


3 25.1 0.1 0 0 100 Step Gradient 

4 30 16.9 0 0 100 Isocratic 

7 30.1 0.1 86 14 0 Step Gradient 

8 42 14.9 86 14 0 Re-equilibration 

HPLC FLOW RATE: 0.35 mL/min, COLUMN TEMP.: 38 ºC 

a-Amino-n-Butyric Acid 

Cystathionine 

Methionine 

Isoleucine 

Leucine 

Tyrosine 

Phenylalanine 

5 10 15 20 

25 

min


STANDARD LITHIUM CATION-EXCHANGE COLUMN 0393250 (3.0 X 250 mm) 

CATALOG NUMBER 0393250 


USE FOR PHYSIOLOGICAL FLUIDS, COMPLEX MATRICES 


1 

2 

3 

44 

20 

22 

40 

39 

5 

8 

7 

10 

9 

15 

12 13 

11 

14 

1718 

16 19 

30 

23 2425 

21 

26 27 

28 29 

31 

32 

34 

35 

33 

37 38 

41 

42 

43 

45 

23 

4 

6 

36 

0 20 40 60 80 100 120 140 160 180 

min 

1 Phosphoserine 

2 Taurine 

3 Phosphoethanolamine 

4 Urea 


6 Hydroxyproline 

7 Threonine 

8 Serine 

9 Asparagine 


11 Glutamine 

12 Sarcosine 

13 α−Aminoadipic acid 

14 Proline 

15 Glycine 

16 Alanine 

17 Citrulline 

18 α−Amino-n-butyric acid 

19 Valine 

20 Cystine 

21 Methionine 


23 Isoleucine 

24 Leucine 

25 Norleucine 

26 Tyrosine 


28 β−Alanine 

29 β−Amino-i-butyric acid 


31 γ−Aminobutyric acid 

32 Tryptophan 



35 Ammonia 

36 Creatinine 

37 Ornithine 

38 Lysine 

39 Histidine 



42 Carnosine 

43 Anserine 

44 α−Amino−β− 

guanidino propionic acid 

45 Arginine 

CONDITIONS 

TIME (Min) Li275 % Li750 % RG003 % 

0 100 0 0 

17 100 0 0 

65 65 35 0 

128 0 100 0 

145 0 100 0 

185 0 94 6 

185.1 100 0 0 

210 100 0 0 

FLOW RATE: 0.3 mL/min, COLUMN TEMP.: 40 ºC, 

INJECTION VOLUME: 10 μL OF 0.25 μmole/mL STD. 



10/11

Met 

Tyr 

Phe 

Met 

Tyr 

Leu + Ile 

Leu + Ile 

Phe 


HIGH-EFFICIENCY CATION-EXCHANGE COLUMN (4.0 X 50 mm) 



USE FOR PKU/MSUD SCREENING OF PHYSIOLOGICAL FLUIDS 


0 

1 2 

3 4 5 6 7 8 

min 

Normal Neonate serum 

0 

1 2 3 4 5 6 7 8 

min 

Neonate serum with elevated Phenylalanine 

CONDITIONS 

TIME (Min) Li357 % RG003 % 

0 100 0 

8 100 0 

8.1 0 100 

10 0 100 

10.1 100 0 

16 100 0 


INJECTION VOLUME: 10 μL 

LITHIUM COLUMNS CATALOG INFORMATION 


DESCRIPTION 

0354050 High-efficiency Cation-exchange Column for PKU and MSUD screens 4.0 x 50 mm 

0354675T 

0354100T 

70-min High-efficiency Lithium Ion-exchange Column, 4.6 x 75 mm 

High-efficiency Lithium Cation-exchange Column 4.0 x 100 mm &1700-0070 amino acid test mixture 

0393250 Standard Lithium Cation-exchange Column 3.0 x 250 mm &1700-0070 amino acid test mixture 

0352020 Lithium Guard Column, 2.0 x 20 mm (for use with 0353100T or 0354050 column) 

0392020 Lithium Guard Column, 2.0 x 20 mm (for use with 0393250 column) 

GARD COLUMN PROTECTION SYSTEM (FOR USE WITH ANY CATION-EXCHANGE COLUMN) 


DESCRIPTION 





SODIUM AMINO ACID ANALYSIS COLUMNS 

Ion-exchange chromatography followed by post-column 

derivatization has been a method of choice for amino acid 

analysis for many years. Pickering ion-exchange columns 

allow you to obtain consistent results with sensitivity, stability, 

selectivity and speed. Sodium columns and buffers systems are 

designed for amino acids analysis of hydrolyzed samples. 

Post-column Conditions For Amino Acids Analysis: 

Reagent: Trione® 



Detection: 

UV-Vis Detector: λ=570 nm for primary amino acids 

λ=440 nm for secondary amino acids 

or 

Reagent: 300 mg of OPA, 2 g Thiofluor, 3 mL of 30 % 




Detection: 


330 nm, λ em 

465 nm 




method. 




Pickering Laboratories recommends six different gradient 

conditions depending on the column and type of sample. 

Use the program recommended on the column data sheet 

for the initial testing. 

The column oven temperature programming gives additional 

flexibility when optimizing methods. Using temperature 

gradient allows to improve separation, shorten analysis 

time and fine-tune the method for detecting compounds 

of interest. 

25 



10/11


30-MIN HIGH-EFFICIENCY SODIUM CATION-EXCHANGE COLUMN (4.6 X 110 mm) 


USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM 

USE FOR PROTEIN AND COLLAGEN HYDROLYSATE 


HPLC PROGRAM 

TIME Na315 % Na740 % RG011 % 

0 100 0 0 

7.3 100 0 0 

15 53 47 0 

15.1 0 100 0 

28 0 100 0 

28.1 0 0 100 

30 0 0 100 

30.1 100 0 0 

39 100 0 0 



TIME TEMP ºC 

0 46 

3 46 

10 55 

22 70 

27 70 

28 46 

REAGENT FLOW RATE: 0.5 mL/min 

8 

17 

1 2 3 

6 7 

9 

10 

11 13 

12 

14 

15 

16 

Protein 

Hydrolysate 

4 

20 

18 

19 

5 

0 10 20 30 

1 Aspartic Acid 8 Cystine 


2 Threonine 9 Valine 

16 Lysine 

3 Serine 

10 Methionine 17 Histidine 

4 Glutamic Acid 11 Isoleucine 18 Ammonia 

5 Proline 

12 Leucine 

19 Tryptophan 

6 Glycine 

13 Norleucine 20 Arginine 

7 Alanine 

14 Tyrosine 

min 


column oven or HPLC column oven with temperature gradient capabilities.


HIGH-EFFICIENCY CATION-EXCHANGE COLUMN (4.0 X 150 mm) 


USE WITH GARD COLUMN PROTECTION SYSTEM OR SODIUM GUARD COLUMN 1193020 (3.0 X 20 mm) 

USE FOR OXIDIZED HYDROLYSATE, PROTEIN HYDROLYSATE, COLLAGEN HYDROLYSATE 


23 

3 Aspartic Acid 

4 Threonine 

5 Serine 

6 Glutamic Acid 

7 Proline 

8 Glycine 

9 Alanine 

11 Valine 

13 Isoleucine 

24 

3 4 5 6 

7 

8 

9 

11 

13 14 

16 

0 10 20 30 40 50 

18 

19 

20 

14 Leucine 


18 Lysine 

19 Ammonia 

20 Histidine 

22 Arginine 

23 Cysteic Acid 

24 Methionine Sulfone 

Oxidized 

Hydrolysate 

22 

min 

CONDITIONS FOR OXIDIZED SAMPLES 1154150T 

TIME (Min) Na270 % Na740 % RG011 % 

0 100 0 0 

14 100 0 0 

32 20 80 0 

32.1 0 100 0 

56 0 100 0 

56.1 0 0 100 

58 0 0 100 

58.1 100 0 0 

70 100 0 0 



27 

10 

1112 

9 

18 

20 

13 

14 

15 16 

17 

8 

11 

9 12 18 

13 14 

15 16 

20 

Protein 

Hydrolysate 

1 

3 4 

5 

6 

8 

10 

Collagen 

Hydrolysate 

3 4 5 6 

7 

19 

21 

22 

2 

7 

19 

22 

0 10 20 30 40 50 

min 

0 10 20 30 40 50 

min 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

12 

Methionine-D,L,-Sulfoxide 

trans-4-Hydroxy-L-Proline 

Aspartic Acid 

Threonine 

Serine 

Glutamic Acid 

Proline 

Glycine 

Alanine 

Cystine 

Valine 

Methionine 

13 

14 

15 

16 

17 

18 

19 

20 

21 

22 

Isoleucine 

Leucine 

Tyrosine 

Phenylalanine 

D,L & allo-Hydroxylysine 

Lysine 

Ammonia 

Histidine 

Tryptophan 

Arginine 

CONDITIONS FOR PROTEIN & 

COLLAGEN HYDROLYSATE SAMPLES 


0 100 0 0 

10 100 0 0 

30 0 100 0 

53 0 100 0 

53.1 0 0 100 

55 0 0 100 

55.1 100 0 0 

67 100 0 0 





10/11


HIGH-EFFICIENCY SODIUM CATION-EXCHANGE COLUMN (4.0 X 150 mm) 


USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR SODIUM GUARD COLUMN 1193020 (3.0 X 20 mm) 

USE FOR PROTEIN HYDROLYSATE, COLLAGEN HYDROLYSATE 


10 

5 

3 4 

9 

11 

12 

13 

14 

18 

20 

8 

Protein Hydrolysate 

6 

15 16 

19 

22 

21 

7 

5 

3 

4 

9 

8 

11 

12 

13 

14 

18 

20 

10 


1 

6 

15 

16 17 

19 

22 

2 

7 

0 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

5 10 15 20 25 30 35 40 45 50 55 60 

min 

Methionine-D,L,-Sulfoxide 

trans-4-Hydroxy-L-Proline 

Aspartic Acid 

Threonine 

Serine 

Glutamic Acid 

Proline 

Glycine 

Alanine 

Cystine 

Valine 

12 

13 

14 

15 

16 

17 

18 

19 

20 

21 

22 

Methionine 

Isoleucine 

Leucine 

Tyrosine 

Phenylalanine 

D,L & allo-Hydroxylysine 

Lysine 

Ammonia 

Histidine 

Tryptophan 

Arginine 

CONDITIONS 

TIME (Min) 1700-0112 % Na740 % RG011 % 

0 100 0 0 

12 100 0 0 

34 0 100 0 

53 0 100 0 

53.1 0 0 100 

55 0 0 100 

55.1 100 0 0 

67 100 0 0 


INJECTION VOLUME: 10 μL OF 0.25 μmole/mL STD.


STANDARD SODIUM CATION-EXCHANGE COLUMN (3.0 X 250 mm) 


USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR SODIUM GUARD COLUMN 1192020 (2.0 X 20 mm) 

USE FOR PROTEIN HYDROLYSATE 


3 

5 

4 

11 

13 

14 

18 

20 

10 

19 

6 

8 

9 

Protein Hydrolysate 

12 

15 

16 

22 

7 

21 

29 

0 

5 10 15 20 25 30 35 40 45 50 55 60 

min 


4 Threonine 

5 Serine 


7 Proline 

8 Glycine 

9 Alanine 


1154110T 

1154150T 

10 Cystine 

11 Valine 

12 Methionine 

13 Isoleucine 

14 Leucine 

15 Tyrosine 


SODIUM COLUMNS CATALOG INFORMATION 

DESCRIPTION 


18 Lysine 

19 Ammonia 

20 Histidine 

21 Tryptophan 

22 Arginine 

30-minute High-efficiency Sodium Cation-exchange Column 4.6 × 110 mm & 1700-0070 amino 

acid test mixture 

High-efficiency Sodium Cation-exchange for protein, collagen and oxidized feed hydrolysates 

Column 4.0 × 150 mm & 1700-0070 amino acid test mixture 

1154150 

High-efficiency Sodium Cation-exchange for protein and collegen hydrosates Column 

4.0 × 150 mm & 1700-0070 amino acid test mixture 

1193250 Standard Sodium Cation-exchange Column 3.0 × 250 mm & 1700-0070 amino acid test mixture 



CONDITIONS FOR PROTEIN HYDROLYSATE SAMPLES 


0 100 0 0 

10 100 0 0 

32 0 100 0 

56 0 100 0 

56.1 0 0 100 

58 0 0 100 

58.1 100 0 0 

70 100 0 0 



GARD COLUMN PROTECTION SYSTEM 

(FOR USE WITH ANY CATION-EXCHANGE COLUMN) 


DESCRIPTION 



1700-3100 GARD Holder 



10/11


CARBAMATE PESTICIDE ANALYSIS COLUMNS 

Pickering Laboratories carbamate columns are guaranteed to produce the separation of carbamate residues, specified by 

EPA and AOAC methods. For C 18 

columns, two water/methanol gradient conditions are available for analysis of methanolic 

and water samples. Expanded resolution C 8 

column is capable of separating as many as 23 carbamates with either 

water/methanol or water/acetonitrile gradients. The difference in selectivity between Methanol and Acetonitrile protocols 

could be used for confirming peak identification. 

Post-column conditions for pesticide analysis: 

Reagent 1: Hydrolysis reagent CB130 or CB130.2 

Reagent 2: 100 mg of OPA, 2 g Thiofluor 

in 950 mL of CB910 

Reactor 1: 100 °C, 0.5 mL 

Reactor 2: ambient. 0.1 mL 

Reagents fl ow rate: 0.3 mL/min 

Detection: 


330 nm, λ em 

465 nm 




method. 




CARBAMATE COLUMN 1846250 (4.6 X 250 mm), C 18 , 5 µm 

1 

4 

2 

3 

6 

5 

4 ng of Carbamates in 150 μL 

9 

11 

7 

8 12 

10 

0 5 10 15 20 25 30 35 40 45 

min 

CONDITIONS FOR 

AQUEOUS SAMPLES 

TIME (Min) WATER % MeOH % 

0 100 0 

1 100 0 

1.1 82 18 

36 30 70 

39 30 70 

39.1 0 100 

41 0 100 

41.1 100 0 

55 100 0 

FLOW RATE: 1 mL/min, 

COLUMN TEMP.: 42 ºC, 

INJECTION VOLUME: Up to 400 μL 

0 

1 

2 

3 

4 

4 

2 

1 

6 

3 

5 


9 

7 

10 

8 

11 12 

5 10 15 20 25 30 35 40 45 50 

min 

Aldicarb sulfoxide 

5 3-Hydroxycarbofuran 9 Carbaryl 

Aldicarb sulfone 

6 Aldicarb 

10 1-Naphthol 

Oxamyl 

7 Propoxur 

11 Methiocarb 

Methomyl 

8 Carbofuran 

12 BDMC (internal standard) 


METHANOLIC SAMPLES 


0 85 15 

1 85 15 

44 25 75 

44.1 0 100 

49 0 100 

49.1 85 15 

57 85 15 



INJECTION VOLUME: 10 μL




AQUEOUS SAMPLES 


0 100 0 

1 100 0 

1.1 88 12 

26 30 70 

26.1 0 100 

28 0 100 

28.1 100 0 

38 100 0 

0 

4 

1 

2 

3 

5 


6 

7 

8 

5 10 15 20 25 30 

min 

9 

10 11 

12 



INJECTION VOLUME: Up to 250 μL 


METHANOLIC SAMPLES 


0 85 15 

0.5 85 15 

28.5 30 70 

28.6 0 100 

33.5 0 100 

33.6 85 15 

41 85 15 




0 

1 

2 

3 

4 

5 

6 

7 


4 

9 

6 

1 2 3 

5 

7 

8 

10 

11 

12 

5 10 15 20 25 30 

min 


8 Carbofuran 


9 Carbaryl 

Oxamyl 

10 1-Naphthol 

Methomyl 

11 Methiocarb 

3-Hydroxycarbofuran 12 BDMC (internal standard) 

Aldicarb 

Propoxur 

31 



10/11




WATER/METHANOL GRADIENT 


0 85 15 

2 85 15 

42 30 70 

46 30 70 

46.1 0 100 

51 0 100 

51.1 85 15 

59 85 15 

FLOW RATE: 0.8 mL/min, 


INJECTION VOLUME: 10 μL for 

methanolic and up to 400 μL for 

aqueous samples 

0 

4 

1 

6 

2 

3 

5 


9 

7 10 

8 

11 

12 

5 10 15 20 25 30 35 40 45 50 

min 


WATER/ACETONITRILE 

GRADIENT 

TIME (Min) WATER % ACN % 

0 90 10 

2 90 10 

46 49 51 

46.1 30 70 

49 30 70 

49.1 90 10 

59 90 10 






0 

40 pg of Carbamates in 400 μL 

9 

1 

3 

4 

6 

11 

5 

7 

12 

8 

10 

2 

5 10 15 20 25 30 35 40 45 50 

min 

1 

2 

3 

4 



Oxamyl 

Methomyl 

5 

6 

7 

8 

3-Hydroxycarbofuran 

Aldicarb 

Propoxur 

Carbofuran 

9 

10 

11 

12 

Carbaryl 

1-Naphthol 

Methiocarb 

BDMC (internal standard)


SEPARATION OF 23 CARBAMATES USING 0840250 COLUMN (4.0 X 250 mm) 


WATER/METHANOL GRADIENT 


0 85 15 

2 85 15 

42 30 70 

46 30 70 

46.1 0 100 

51 0 100 

51.1 85 15 

59 85 15 






1 Aldicarb sulfoxide 

2 Aldicarb sulfone 

3 Oxamyl 

4 Methomyl 

5 3-Hydroxy 

6 Aldicarb 

7 Propoxur 

8 Carbofuran 

9 Carbaryl 

10 Methiocarb 

11 BDMC internal standard 

12 Butocarboxim sulfoxide 

13 Butocarboxim sulfone 

14 Ethiofencarb sulfoxide 

15 Ethiofencarb sulfone 

16 Butocarboxim 

17 Ethiofencarb 

18 Thiofanox sulfoxide 

19 Thiofanox sulfone 

20 Methiocarb sulfoxide 

21 Methiocarb sulfone 

22 3-Ketocarbofuran 

23 Thiofanox 

N 1-Naphthol 

4 

9 

1 

6 

2 

3 

5 

7 N 

8 

10 

11 

4 

20 

12 

13 

2 

14 

1 

3 

9 

6 

16 

7 

15 

17 

19 

21 

5 

11 

18 

22 8 

10 

23 

5 10 15 20 25 30 35 40 45 min 

33 


CARBAMATE COLUMNS CATALOG INFORMATION 

DESCRIPTION 


, expanded resolution, 4.0 × 250 mm & 

1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL 


, high resolution/capacity, 4.6 × 250 mm & 

1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL 


, rapid analysis, 4.6 × 150 mm & 1700-0063 

Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL 

18ECG001 Guard Cartridge Holder with 3 guard cartridges 

18ECG002 Guard Cartridges, 2/pk. 



10/11


GLYPHOSATE HERBICIDE ANALYSIS COLUMN 

Pickering Laboratories cation-exchange Glyphosate column 

is designed and tested for analysis of Glyphosate and its 

primary metabolite Aminomethylphosphonic acid (AMPA). 

This short, isocratic method provides separation of the 

peaks of interest. Reproducible performance is guaranteed 

run-to-run and column-to-column. 

Post-column Conditions For Glyphosate Analysis: 

Reagent 1: Oxidizing reagent—100 μL of 5 % Sodium 

Hypochlorite in 950 mL of GA116 

Reagent 2: 100 mg of OPA, 2 g Thiofluor in 950 mL of GA104 

Reactor 1: 36 °C, 0.5 mL 

Reactor 2: ambient. 0.1 mL 

Reagents Flow Rate: 0.3 mL/min 

Detection: 


330 nm, λ em 

465 nm 

GLYPHOSATE COLUMN 1954150 (4.0 X 150 mm) K+ CATION-EXCHANGE. 

TO BE USED WITH CATION-EXCHANGE GARD COLUMN PROTECTION 

SYSTEM OR GUARD COLUMN (3.0 X 20 mm), K+ CATION-EXCHANGE 

Glyphosate 

AMPA 

0 10 20 min 

Glyphosate Test Mixture, 2.5 μg/mL 

CONDITIONS 

TIME (Min) K200 % RG019 % 

0 100 0 

15 100 0 

15.1 0 100 

17 0 100 

17.1 100 0 

27 100 0 





GLYPHOSATE COLUMNS CATALOG INFORMATION 

DESCRIPTION 

1954150 

Cation-exchange Column for Glyphosate analysis, 4 × 150 mm & 

1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL 

1953020 Cation-exchange Guard Column, 3.0 × 20 mm 

1952150 

1954250 

Micro Cation-exchange Column, K+ form, 2.1 × 150 mm & 1700- 

0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL 

Column, Glyphosate 4 x 250 mm & 1700-0080 Glyphosate test 

mixture, 2.5 µg/mL, 1.5 mL 


GARD COLUMN PROTECTION SYSTEM 

(FOR USE WITH ANY CATION-EXCHANGE COLUMN) 

DESCRIPTION 

1700-3102 

Cation-exchange GARD Assembly: Includes holder and 

2 replaceable GARDS 




ALKION COLUMN 

The stationary phase in the ALKION column is a rigid, 

non-porous polymeric phase that is surface sulfonated. The 

two modes of separation are ion-exchange and moderate 

partitioning. The low capacity of the ion-exchange resin 

makes it an ideal phase for the separation of strongly basic 

and positively charged compounds. The reversed-phase 

character allows for discrimination between closely related 

species. 

1 

2 

3 4 

5 

The unique properties of this column allow using it for a 

wide range of applications. 

ALKION COLUMN ANALYSIS OF BIOGENIC AMINES 

Post-column Conditions: 

Reagent 1: 300 mg of OPA, 2 g Thiofluor, 3 mL of 30 % 

Brij® 35 in 950 mL of OD104 



Detection: 


330 nm, λ em 

465 nm 

0 

1 

2 

3 

5 10 15 20 

min 

Histamine 

Putrescine 

Cavaverine 

4 

5 

CONDITIONS 

Spermidine 

Spermine 

TIME (Min) K600 % K563 % K130 % 

0 100 0 0 

6 100 0 0 

15 0 100 0 

21 0 100 0 

21.1 0 0 100 

23 0 0 100 

23.1 100 0 0 

29 100 0 0 




35 

Histamine 

5 Days Old 

5 Days Old 

Putrescine 

Cadaverine 

Fresh 

0 2 4 6 8 10 

min 

Ahi Tuna Fillet extracted per FDA Method 

Histamine 

Putrescine 

Cadaverine 

Fresh 

0 2 4 6 8 10 

min 

Mahi-Mahi extracted per FDA Method 

CONDITIONS 

TIME (Min) K600 % K130 % 

0 100 0 

12 100 0 

12.1 0 100 

15 0 100 

15.1 100 0 

20 100 0 






10/11


ALKION COLUMN ANALYSIS OF AMINOGLYCOSIDE 

ANTIBIOTICS IN FEEDS 


Reagent 1: 300 mg of OPA, 2g Thiofluor, 3 mL of 30 % 

Brij® 35 in 950 mL of OD104 



Detection: 


330 nm, λ em 

465 nm 

Apramycin 

CONDITIONS FOR APRAMYCIN ANALYSIS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 67 33 0 

5 67 33 0 

15 14.7 7.3 78 

20 14.7 7.3 78 

20.1 0 22 78 

21 0 22 78 

21.1 67 33 0 

28 67 33 0 




0 

5 10 15 20 25 30 

min 

Gentamicin 

Complex 

CONDITIONS FOR GENTAMICIN ANALYSIS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 43 31 26 

20 9 13 78 

30 9 13 78 

30.1 0 22 78 

31 0 22 78 

31.1 43 31 26 

38 43 31 26 




0 

5 10 15 20 25 30 

min 

Neomycin 

CONDITIONS FOR NEOMYCIN ANALYSIS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 60 40 0 

15 13.2 8.8 78 

25 11 11 78 

25.1 0 22 78 

26 0 22 78 

26.1 60 40 0 

32 60 40 0 




0 

5 10 15 20 25 30 

min


CONDITIONS FOR SEPARATION OF APRAMYCIN 

GENTAMICIN AND NEOMYCIN 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 60 40 0 

15 13.2 8.8 78 

15.1 12 10 78 

30 12 10 78 

30.1 0 22 78 

31 0 22 78 

31.1 60 40 0 

37 60 40 0 




Neomycin 

37 

Gentamicin 

Complex 

Apramycin 

0 

5 10 15 20 25 30 

min 



10/11


ALKION COLUMN ANALYSIS OF PARAQUAT AND 

DIQUAT IN WATER 


Reagent 1: 300 mg/L of Sodium Dithionite in 

0.3 M Sodium Hydroxide 



Detection: 

UV-Vis Detector: λ 395 nm for PQ, λ 378 nm for DQ 

CONDITIONS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % i-PROPANOL % 

0 31.5 53.5 0 15 

10 28 47 10 15 

15 28 47 10 15 

15.1 0 85 10 5 

17 0 85 10 5 

17.1 31.5 53.5 0 15 

23 31.5 53.5 0 15 



INJECTION VOLUME: up to several mL 

DQ 

ALKION COLUMN ANALYSIS OF STREPTOMYCIN 


Reagent 1: 0.75 N Sodium Hydroxide 

Reactor 1: ambient, 0.1 mL 

Reagent 2: 0.6 % Ninhydrin 



Detection: 


395 nm, λ em 

500 nm 

or 

Reagent 1: Oxidizing Reagent: 100 μL of 5 % 

Sodium Hypochlorite in GA116 


Reagent 2: 300 mg of OPA, 2 g Thiofluor, 3 mL of 30 % 



Detection: 


330 nm, λ em 

465 nm 

PQ 

PQ=Paraquat 

DQ=Diquat 

CONDITIONS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 60 40 0 

10 60 40 0 

10.1 48 48 4 

15 48 48 4 

15.1 60 40 0 

21 60 40 0 



INJECTION VOLUME: up to several μL 

Streptomycin 

6 

8 10 12 

min 

NOTE: Above chromatogram is approximately 1 ppm in 200 µL. 

ALKION COLUMN CATALOG INFORMATION 


DESCRIPTION 

9410917 ALKION Cation-exchange column, K form, 4.0 × 150 mm 

9493020 ALKION Guard column, K form, 3.0 × 20 mm 

0 5 10 15 

min


PARALYTIC SHELLFISH TOXINS 

ANALYSIS COLUMN 

LC Condition: 

Flow Rate: 0.8 mL/min, column temperature 40 °C 


Reagent 1: Periodic acid (7 mM) in 50 mM Potassium 

Phosphate buffer, pH 9.0 


Reagent 2: 0.5 M Acetic Acid 



Detection: 


330 nm, λ em 

390 nm 

Mobile Phases: 

A. For C1–C4 toxins: 

Tetrabutylanmmonium phosphate (1 mM) adjusted to 

pH 5.8 with acetic acid 

B. For GTX-1 to GTX-6, dcGTX2, and dcGTX3: 

Sodium 1-heptanesulfonate (2 mM) in 10 mM 

Ammonium phosphate, pH 7.1 

C. For STX, neoSTX, and dcSTX: 

Sodium 1-heptanesulfonate (2 mM) in 10 mM 

Ammonium phosphate, pH 7.1-Acetonitrile (100+5) 

39 

neoSTX 

dcSTX 

STX 

6 8 10 12 14 min 


PARALYTIC SHELLFISH TOXINS CATALOG INFORMATION 

DESCRIPTION 

0846150 Reversed-phase column for analysis of paralytic shellfish toxins, C 8 

, 

4.6 × 150 mm 

18ECG001 Guard Cartridge Holder with 3 cartridges 




10/11


MYCOTOX MYCOTOXIN ANALYSIS COLUMN 

MULTI-RESIDUE MYCOTOXIN ANALYSIS 

Single Run Analysis of Deoxynivalenol, Aflatoxins, Ochratoxin A, 

Zearalenone and Fumonisin 

Sample Extraction and Clean-up 

25 g of finely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of filtered extract 

is diluted with 70 mL of Phosphate Buffered Saline (PBS). Aflatoxins, Zearealenone and Ochratoxin A are isolated using 

AOZ Immunoaffinity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are eluted 

with 2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoaffinity column (Vicam, USA) according to 

the procedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol. 

To isolate DON 3 mL of filtered extract is mixed with 6 mL of Acetonitrile and cleaned with MycoSep 277 column 

(Romer Labs, USA) according to the manufacture’s instructions. The cleaned solution is filtered and 5.5 mL of it is combined 

with eluants from AOZ and FumoniTest columns. The solution is evaporated to 0.5 mL and final volume is adjusted 

to 1 mL with Methanol. 

Analytical Conditions 

Column: MYCOTOX reversed-phase C 18 

, 

4.6 x 250 mm Catalog No. 1612124 

Temperature: 40 ºC 

Flow Rate: 1 mL/min 

Mobile Phase: Sodium Phosphate buffer, pH 3.5 

Catalog No 1700-1108/MeOH/ACN 

Post-Column Conditions 

Post-Column System: Pinnacle PCX 

Reactor Volume: 1.4 mL 

Temperature: 60 ˚C 

Reagent: OPA, Thiofluor, Brij 35® in GA104 

Photochemical Reactor: UVE 

Detection: 

Fluorescence 

Aflatoxins (photochemical derivatization) 

λ ex 

= 365 nm; λ em 

= 455 nm 

Fumonisins (post-column derivatization with OPA) 

λ ex 

= 330 nm; λ em 

= 465 nm 

Ochratoxin A 

λ ex 

= 335 nm; λ em 

= 455 nm 

Zearalenone 

λ ex 

= 275 nm; λ em 

= 455 nm 

UV/Vis 

Deoxynivalenol 

λ=218 nm 

HPLC PROGRAM 

TIME (Min) 1700-1108 ,% METHANOL, % ACETONITRILE, % 

0.0 85 0 15 

5.0 85 0 15 

5.1 57 28 15 

20.0 57 28 15 

23.0 40 60 0 

40.0 40 60 0 

50.0 20 0 80 

60.0 20 0 80


MYCOTOXIN CALIBRATION STANDARD 

FLD Signal 

reagent ON 

reagent OFF 

UV signal 

DON 

FB1 

reagent ON 

FB2 

G1 

G2 B2 

B1 

Ochra 

ZON 

0 10 20 30 40 50 min 

Chromatogram of a standard solution of mycotoxins. Concentrations of toxins (ng/mL): deoxynivalenol (DON) 930, 

aflatoxin B1 4.5, aflatoxin B2 1.6, aflatoxin G1 4.7, aflatoxin G2 2, ochratoxin A 92, zearalenone 481, fumonisin B1 

474, and fumonisin B2 627. 

41 

FLD Signal 

reagent ON 

FB1 

reagent OFF 

UV signal 

reagent ON 

FB2 

DON 

G1 

G2 B2 

B1 

Ochra 

ZON 

0 10 20 30 40 50 min 

Chromatogram of a corn grain sample naturally contaminated with fumonisins FB1 and FB2 and spiked with DON; 

aflatoxins B1, B2, G1, and G2; ochratoxin A; and zearalenone. Concentrations of toxins in the sample (ng/g): 

deoxynivalenol 930, aflatoxin B1 5.0, aflatoxin B2 1.7, aflatoxin G1 5.1, aflatoxin G2 2.2, ochratoxin A 102, 

zearalenone 529, fumonisin B1 1838, and fumonisin B2 1107. 



10/11


AFLATOXINS ANALYSIS 

Aflatoxins are naturally occurring toxins belonging to the class Mycotoxins. They are produced by fungi and occur 

in peanuts, peanut meal, cotton-seed meal, wheat, milk and many other foods and feeds. 

The most important feature of the post-column method described below is that all six Aflatoxins are detectable 

at the same fluorescence emission wavelength in a single isocratic HPLC analysis. 

LC Conditions: 


Flow Rate: 1.0 mL/min, column temperature 42 °C, Reagent: I 2 

100 mg/L in water 

injection volume 10 μL 


Mobile Phase: Methanol/acetonitrile/water: 


(22:22:56), isocratic 

Detection: 

Injection: 10 μL in Methanol 

Fluorometer: λ 

5 ng B 1 

& G 

ex 

365 nm, λ em 

430 nm 

1.5 ng B 2 

& G 2 

1.25 ng M 1 

& M 2 

G1 B2 

B1 

M2 

M1 

G2 

0 

2 4 

6 

8 10 12 14 

min 

MYCOTOX COLUMN CATALOG INFORMATION 


DESCRIPTION 

1612124 MYCOTOX Reversed-phase column, C 18 

, 4.6 × 250 mm 


18ECG002 Guard Cartridges, 2/pk.


POLYETHER ANTIBIOTICS ANALYSIS COLUMN 

Polyether antibiotics, such as Monensin, Narasin and Salinomycin, found in raw material, premix, liquid supplements and 

final feeds, are best quantified using HPLC with post-column derivatization. Because of selectivity of post-column reaction, 

almost no sample clean-up is needed. 

LC Conditions: 

Flow Rate: 0.7 mL/min, column temperature 40 °C, 

injection volume 10 μL 

Mobile Phase: Methanol/5 % Acetic Acid in 

Water (90:10), isocratic 

Feed Extract 

2 

Post-column Conditions 

(non-metallic post-column derivatization system is required): 

Reagent 1: Concentrated Sulfuric acid / Methanol (4:96 v/v) 

Reactor 1: Ambient, 0.1 mL 

Reagent 2: 60 g of Vanillin (or p-dimethylaminobenzaldehyde) 

in 950 mL of Methanol 



NOTE: Using 2-reagent system for this application greatly extends reagents life. 

One-reagent method and post-column derivatization 

system are also available. 

Detection: 

UV-Vis detector: Vanillin λ=520 nm, DMAB λ=450 nm 

1 

43 

2 

3 

Standard 

1 

0 

5 

10 15 

1 Monensin B 2 Monensin A 3 Narasin A 

min 

POLYETHER ANTIBIOTICS CATALOG INFORMATION 


DESCRIPTION 

2381750 Polyether reversed-phase column, C 18 

, 4.6 × 250 mm 





10/11


VOGLIBOSE ANALYSIS COLUMN 

METHOD 


Column: Amino column, 

4.6x250 mm, 

Catalog number 1446250 

Temperature: 35 °C 

Flow Rate: 0.6 mL/min 

Mobile Phase: Sodium phosphate buffer, 20 mM 

pH 6.5 / Acetonitrile (37 : 63) 

Injection Volume: 50 μL 


Crush 5 tablets and mix with 25 mL of mobile phase. 

Sonicate for 10 min and filter liquid portion through 

0.45 μm filter. Put in HPLC autosampler vial and inject 50 μL. 


Post-column System: Pinnacle PCX 

Heated Reactor Volume: 3.5 mL 


Cooling Coil: 0.15 mL (at room temperature) 

Reagent: Taurine (6.25 g), Sodium Periodate (2.56 g) 

in 1000 mL of water 


Detection: FLD 

λ ex 

: 350 nm, λ em 

: 430 nm 

Voglibose 

Voglibose 

0 5 10 15 20 25 

min 

0 5 10 15 20 25 

min 

Chromatogram of Voglibose Standard, 

50 ppm, 50 μL Injection 

Chromatogram of Voglibose Tablets (Volix TM , 0.2 mg), 

50 μL Injection 

VOGLIBOSE ANALYSIS CATALOG INFORMATION 


DESCRIPTION 

1446250 Amino Column, 4.6 × 250 mm, 5µm


ANION-EXCHANGE COLUMN 

NITRITE & NITRATE ANALYSIS 

AOAC Official method 993.03 1 for the analysis of nitrate involves reduction using spongy Cadmium which is toxic and 

carcinogenic. FDA improved on this method by using Vanadium(III) chloride and heat 2 for the post-column reduction of 

nitrate to nitrite. Nitrite reacts with this modified Griess reagent to produce a red chromophore with maximal absorbance 

at 535nm. Pickering Laboratories Inc. has further improved this method by substituting the corrosive hydrochloric acid with 

methane sulfonic acid. 

METHOD 


Column: Anion exchange column 

4.6 x 150 mm, 



Sample Injection Volume: 10 μL 

LC Flow Rate: 1 mL/min 

Mobile Phase: 20 mM sodium chlorate and 

10 mM dihydrogensodium phosphate 

0.12 

0.10 

Nitrite 


Post-column System: Pinnacle 


Reactor Temperature: 100 °C 


Reagent: (i) 1 % Vanadium(III) chloride in 20 % methanesulfonic acid 

(ii) 1 % m-Nitro aniline in 20 % methanesulfonic acid 

(iii) 1 % N-(1-Naphthyl)ethylenediamine 

dihydrochloride in 20 % methanesulfonic acid 

Mix 50 mL of (i) and (ii), and 1.25 mL of (iii) and 

dilute to 250 mL using 20 % methanesulfonic acid 

Detection: UV/VIS, λ max 

= 535 nm 


To 5 g of baby food in a 50 mL centrifuge tube, add 25 mL 

of 50-60 °C water (for vegetables) or 15 mM Sodium acetate 

(for fruits) and shake for 10 min. Add 12.5 mL of acetonitrile 

and make up the volume to 50 mL using water (for vegetables) 

and sodium acetate (for fruits). Centrifuge the mixture 

for 15 mins at 5000 rpm. Filter the supernatant through a 

0.45 μ nylon filter and dilute to fall within the linear range. 

NOTES 

Post-column reagent solutions are stored in plastic or Tefl on containers. 

All solutions are fi ltered through 0.45 µ nylon fi lter before use. 

Nitrate/Nitrite standards should be checked prior to use for oxidation. 

Sample pH should be checked to determine the choice of extraction solution 

since acidic pH facilitates the conversion of nitrite to nitrate. 

45 

AU 

0.08 

0.06 

0.04 

0.02 

0.00 

Nitrate 

2.00 4.00 6.00 8.00 10.00 12.00 14.00 

min 

20 ppm Nitrite and Nitrate Standard 

ACKNOWLEDGMENTS 

John A. Casanova, Food and Drug 

Administration, 60 8 th Street, Atlanta, 

GA 30309 

REFERENCES 

1. AOAC- Offi cial Methods of Analysis 

of AOAC International (2000) 17 th Ed., 

Section 50.1.11. 

Use of Griess Reagents Containing 

Vanadium (III) for the Post-column 

Derivatization and Simultaneous 

Determination of Nitrite and Nitrite in 

Baby Food, John A. Casanova, Lois K. 

Gross, Sarah E. McMullen and Frank 

Schenck, Food and Drug Administration, 

60 8 th Street, Atlanta, GA 30309. 



10/11


ANALYSIS OF BROMATE IN DRINKING WATER 

Bromate is a disinfection by-product that is formed when Ozone reacts with naturally occurring Bromide in drinking 

water. Bromate is a known animal carcinogen and has also been listed as a group 2B toxin: probable human carcinogen. 

The USEPA Method 300.1 employs conductivity as the means of detection which works well for most anions. However, 

the method is non-specific and coeluting interferences cannot be identified. The more recent USEPA Method 317.0 

utilizes a Bromate specific reagent in a post-column reaction. This allows for a very specific and sensitive assay for 

Bromate in complex matrices. 

METHOD 


Column: Anion exchange column 

4.6 x 150 mm, 





Mobile Phase: 9 mM Na 2 

CO 3 


Post-column system: Pinnacle PCX 


Reactor Temperature: 60˚C 

Reagent: 

o–Dianisidine dihydrochloride (Add 40 mL of 70 % HNO 3 

to 300mL deionized water in a 500mL volumetric flask. Dissolve 

2.5g KBr in this solution. Dissolve 250 mg of 

o–Dianisidine dihydrochloride in 100 mL of Methanol and 

add to the Nitric acid/KBr solution and dilute to volume.) 


Detection: UV/VIS detector, λ max = 450 nm 

uAU 

2.0 

10ppb 

1.5 

8ppb 

1.0 

5ppb 

0.5 

0.0 

2.5ppb 

-0.4 

1.50 1.75 2.00 2.25 2.50 2.75 3.00 

min 

NITRITE/NITRATE & BROMATE ANALYSIS CATALOG INFORMATION 


DESCRIPTION 

0785150 Anion-exchange column, (4.6 x 150 mm), 5 µm


ANALYSIS OF ALENDRONIC ACID IN PHARMACEUTICAL FORMULATIONS 

Alendronic acid or Alendronate Sodium is a bisphosphonate drug that inhibits osteoclast-mediated bone resorption. 

Biphosphates work by binding to the bone and preventing calcium from being removed by osteoclasts. This drug is used 

to prevent and treat osteoporosis as well as several other bone diseases. Alendronate Sodium is sold as tablets or solutions, 

alone or in combination with Vitamin D. 

METHOD 


Column: Anion-exchange, 

4.1x150 mm, 

catalog number 1441150 



Mobile Phase: Sodium Nitrate, 50 mM, 

pH 7.8 / Methanol (60:40) 

Alendronate Sodium 





Reagent: OPA (300 mg), Thiofluor (2g), 

30 % Brij 35 (3 mL) in 950 mL of OD104 



λ ex 

: 330 nm, λ em 

: 465 nm 

0 5 10 

Chromatogram of Fosavance® tablet 

(Alendronate sodium 70 mg, Vitamin D 2800 IU) 

min 

47 


Crush 1 tablet and mix with water. Use enough water to get 

Alendronate Sodium concentration between 300 and 1000 

μg/mL. Heat the solution using a water bath to 65 ºC and 

stir for 30 min. Filter liquid portion through 0.45 μm filter. 

Put filtrate in HPLC autosampler vial and inject 10 μL. 

ALENDRONIC ACID ANALYSIS CATALOG INFORMATION 


DESCRIPTION 

1441150 Anion-exchange column, (4.1 x 150 mm), 5 µm 



10/11

“PURE CHEMICALS DO WHAT THEY ARE SUPPOSED TO EVERY TIME.” 

To guarantee our formulations, we purify all the reagents that go into them, do analytical confirmation 

of purity, validate there are no anomalies in the base line and that the peaks are in the right place. 

REBECCA SMITH / RESEARCH CHEMIST 

DAVID MAZAWA / TECHNICAL SUPPORT CHEMIST 

Automation is a key element 

in reproducibility.


TRIONE® NINHYDRIN REAGENT 

49 

CHROMATOGRAPHIC GRADE 

A PREPARED REAGENT FOR AUTOMATED POST-COLUMN DERIVATIZATION OF PRIMARY AND SECONDARY AMINES 

TRIONE® Ninhydrin reagent is specially formulated for amino acid analysis. It contains Ninhydrin, Hydrindantin 

(reduced Ninhydrin), a Lithium Acetate buffer, and Sulfolane, a water-miscible organic solvent. The solvent is necessary 

to maintain the solubility of both the Hydrindantin and the primary amine product, Ruhemann’s Purple. The buffer is 

required because the reaction is pH dependent. The active ingredients—Ninhydrin and Hydrindantin are required for 

proper development of secondary and primary amines, respectively. 

TRIONE® is so stable that is does not require refrigeration, either in shipment, storage, or in the reservoir. Quantitation is 

consistent from the first to the last mL, so there is no waste. The high signal-to-noise ratio of TRIONE®, when compared 

to DMSO-containing reagents, permits detection sensitivity to be increased with minimum increase in background noise— 

a feature particularly appreciated at sample concentrations of


o-PHTHALALDEHYDE (OPA) 

CHROMATOGRAPHIC GRADE FOR AUTOMATED 

POST-COLUMN DERIVATIZATION OF PRIMARY AMINES 

Primary amines form highly fluorescent adducts when reacted 

with o-Phthalaldehyde (OPA) and a mercaptan under 

basic conditions. 

The products of this reaction, 1-alkyl-2alkylthio-substituted 

isoindoles, exhibit optimal excitation at 330 nm and maximal 

emission at 465 nm. 

Pickering’s OPA is specially prepared to meet the demanding 

requirements of high-sensitivity pre-and post-column 

HPLC derivatization methods. Our repurification process 

actually begins with commercially available 99 % material. 

The entire process is controlled to eliminate trace interfering 

contamination. Lot quality is verified by post-column 

HPLC using a high-sensitivity fluorescence detector. 

o-PHTHALALDEHYDE (OPA) CATALOG INFORMATION 

CATALOG NO. DESCRIPTION QUANTITY 

0120 o-Phthalaldehyde (OPA) each (5 g/bottle) 

NAPHTHALENE-2,3-DICARBOXALDEHYDE 

CHROMATOGRAPHIC GRADE FOR DERIVATIZATION 

OF PRIMARY AMINO GROUPS 

Naphthalene-2,3-dicarboxaldehyde reacts with primary 

amines in presence of a nucleophile (e.g., mercaptan) and 

forms highly fluorescent 1-Alkylbenz-2-thioalkyl[f]isoindole 

derivatives. 

This reagent is especially effective for post-column or precolumn 

derivatization of proteins, peptides and other large 

amines since the derivative is stable and doesn’t internally 

quench. 

Naphthalenedialdehyde can also be used 

as a reagent for chemiluminescence analysis. 

NAPHTHALENE-2,3-DICARBOXALDEHYDE CATALOG INFORMATION 


3700-0100 Naphthalene-2,3-dicarboxaldehyde each (500 mg/bottle) 

VANILLIN 

4-HYDROXY-3-METHOXYBENZALDEHYDE 

CHROMATOGRAPHIC GRADE FOR POST- 

COLUMN ANALYSIS OF POLYETHER ANTIBIOTICS 

Reagent formulation with Vanillin, Methanol and Sulfuric 

acid allows specific post-column analysis of polyether antibiotics, 

such as Monensin, Narasin and Salinomycin. This 

reagent can also be used for the post-column derivatization 

of Sulfa Drugs. Polyether antibiotics are monitored at 520 

nm and Sulfa Drugs at 400 nm. 

Chromatographic Grade Vanillin meets the exacting 

purity requirements for a post-column chemical, ensuring 

that detection at 520 nm will be free of reagent artifacts. 

Because it is sensitive to oxygen, Pickering Laboratories 

Vanillin is sealed under CO 2 

in convenient 30-gram bottles. 

VANILLIN CATALOG INFORMATION 


3700-2200 Vanillin each (30 g/bottle)


p-DIMETHYLAMINOBENZALDEHYDE (DMAB) 

CHROMATOGRAPHIC GRADE FOR POST-COLUMN ANALYSIS 

OF SULFA DRUGS IN FEED AND ANIMAL TISSUES 

p-Dimethylaminobenzaldehyde (DMAB; Ehrlich’s Reagent) 

reacts rapidly with many primary amines to form a 

complex with maximum absorbance at 450 nm. 

Reversed-phase LC followed by post-column derivatization 

is used to determine the levels of Sulfamethazine, 

Sulfathiazole and related compounds in feeds, premixes 

and animal tissues. Although these compounds have a UV 

chromophore, so do most of the other constituents in the 

complex sample matrix. Forming a colored derivative postcolumn 

allows shifting of the detection wavelength into the 

visible range thus greatly enhancing selectivity. 

p-DIMETHYLAMINOBENZALDEHYDE (DMAB) CATALOG INFORMATION 


3700-0400 p-Dimethylaminobenzaldehyde (DMAB) each (5 g/bottle) 

THIOFLUOR 

N,N-DIMETHYL-2-MERCAPTOETHYLAMINE HYDROCHLORIDE – CHROMATOGRAPHIC GRADE 

FOR AUTOMATED POST-COLUMN DERIVATIZATION OF PRIMARY AMINES 

Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan 

under basic conditions. 

The products of this reaction, 1-alkyl-2-thioalkyl-subsitituted isoindoles, exhibit optimal excitation at 330 nm and 

maximal emission at 465 nm. 

Pickering’s Thiofluor, a solid, nearly odorless nucleophile, is a superior substitute for 2-Mercaptoethanol in the preparation 

of OPA reagents. It forms a more stable reagent and a longer-lasting fluorophore with OPA than does 2-Mercaptoethanol, yet 

it has the same fluorescence properties. 

Unlike the volatile 2-Mercaptoethanol, Thiofluor will not migrate through the gas manifold and regulator of the OPA 

reagent pressurization system. 

Two grams of Thiofluor is equivalent to 1 mL of 2-Mercaptoethanol. 

51 

THIOFLUOR CATALOG INFORMATION 


3700-2000 Thiofluor each (10 g/bottle) 

o-PHTHALALDEHYDE (OPA) DILUENT 

CHROMATOGRAPHIC GRADE FOR PREPARATION OF o-PHTHALALDEHYDE REAGENTS 

The derivatization of primary amines with o-Phthalaldehyde and a mercaptan requires basic conditions. To ensure the 

optimum pH of the reaction it is important to use the correct diluent. 

Three application specific diluents are available : CB910 and GA104 for carbamate and glyphosate analyses respectively, and 

OD104 for analysis of amino acids and other amines. 

These borate buffers are produced from starting materials which are free of heavy metals and amines. As with most other 

products of Pickering Laboratories, the quality of the OPA Diluents is verified by actual post-column HPLC analysis. 

o-PHTHALALDEHYDE (OPA) DILUENT CATALOG INFORMATION 


CB910 o-Phthalaldehyde (OPA) Diluent for Carbamate analysis case of 4 (950 mL/bottle) 

GA104 o-Phthalaldehyde (OPA) Diluent for Glyphosate analysis case of 4 (950 mL/bottle) 

OD104 o-Phthalaldehyde (OPA) Diluent for amino acids and amines analysis case of 4 (950 mL/bottle) 



10/11


SERAPREP & URIPREP 

CHROMATOGRAPHIC GRADE REAGENTS FOR THE PREPARATION 

OF NATIVE SAMPLES FOR AMINO ACID ANALYSIS 

Preparation of samples such as serum, urine, other physiological 

fluids, plant extracts, foods and beverages requires control 

of pH and normality, and removal of soluble protein. 

The samples must be held to a narrow pH range between 

2.1 and 2.5, and at the proper Lithium ion concentration to 

ensure reproducibility in the early part of the chromatogram. 

SERAPREP and URIPREP replace commonly used 

protein precipitation reagents such as Acetonitrile, Perchloric 

acid, and Picric acid, and eliminate the need for dialysis, 

ultrafiltration, and the repeated centrifugation steps, followed 

by pH adjustment. 

SERAPREP is used for preparing serum and other 

samples with high buffering capacity, e.g. sardine oil. 

URIPREP is used for urine and other samples with low 

buffering capacity, such as fruit juices, beer and wines. The 

efficiency of protein precipitation and need for post-centrifugation 

pH adjustment of the sample determine which 

reagent is best for your particular sample. 

SERAPREP & URIPREP CATALOG INFORMATION 


SP100 SERAPREP each (250 mL/bottle) 

UP100 URIPREP each (250 mL/bottle) 

HYDROLYSIS REAGENT CB130 


This 0.05 M NaOH/C47 reagent is applied in the first 

stage of post-column carbamate derivatization. At 100 ºC 

the separated carbamates are converted from urethanes to 

Methylamine. The Methylamine can then react with OPA 

and Thiofluor to form the characteristically fluorescent 

isoindole. 

Hydrolysis Reagent contains C47 which prevents the 

precipitation of insoluble minerals (e.g. Ca 2+ , Mg 2+ ) 

from samples of hard drinking water and ground water. 

The C47 additive complexes these minerals and keeps 

them in solution. 

HYDROLYSIS REAGENT CB130.2 


The new Hydrolysis Reagent CB130.2 is formulated according 

to USEPA Method 531.2 and contains 0.075 M 

Sodium Hydroxide. This reagent is applied during the first 

stage of post-column derivatization for the analysis of carbamate 

pesticides. 

Hydrolysis Reagent CB130.2 contains C47, which prevents 

the precipitation of insoluble minerals from samples 

of hard drinking and ground water, and protects your 

instruments from damage. 

HYDROLYSIS REAGENT CATALOG INFORMATION 


CB130 Hydrolysis Reagent for carbamate 

analysis 0.05 M 

case of 4 

(950 mL/bottle) 

CB130.2 

Hydrolysis Reagent for carbamate 

analysis 0.075 M 

case of 4 

(950 mL/bottle)


HYPOCHLORITE DILUENT 

CHROMATOGRAPHIC GRADE FOR THE PREPARATION OF 

THE OXIDIZING REAGENT FOR POST-COLUMN GLYPHOSATE 

HERBICIDE ANALYSIS 

This diluent is used to prepare the oxidizing reagent required 

to convert glyphosate to a primary amine prior to 

reacting it with OPA. The reagent is prepared by adding 

100 μL of 5 % solution of Sodium hyphochlorite to one 

950 mL bottle of Diluent. The pH 11.6 Diluent is formulated 

to ensure pH stability of the mixed stream of column 

effluent and oxidizing reagent. This diluent could also be 

used in fluorescence detection of other secondary amines. 

HYPOCHLORITE DILUENT CATALOG INFORMATION 


GA116 Hypochlorite Diluent for Glyphosate analysis case of 4 


53 

CHLORAC BUFFER 

CHROMATOGRAPHIC GRADE FOR PRESERVATION 

OF AQUEOUS CARBAMATE SAMPLES 

Trace analysis of carbamate insecticide residues in drinking 

water is a mandated requirement in the U.S. Since several 

of the common carbamates—Carbaryl, Oxamyl, Hydroxycarbofuran—are 

labile in water due to oxidation or hydrolysis, 

the samples and standards must be preserved in order to 

obtain valid results. 

ChlorAC Buffer from Pickering Laboratories is a highly 

purified Chromatographic Grade preservative. It is prepared 

from Monochloracetic acid and Potassium acetate to 

EPA specifications. ChlorAC is guaranteed to be free of 

co-eluting interferences for the analytes in EPA 531.1. 

CHLORAC BUFFER CATALOG INFORMATION 


1700-0132 ChlorAC Buffer each (250 mL/bottle) 



10/11


RESTORE 

CHROMATOGRAPHIC GRADE FOR REMOVAL OF METAL ION CONTAMINATION AND RESTORATION 

OF PROPER ION BALANCE IN GLYPHOSATE ANALYTICAL COLUMNS AND GUARDS 

Glyphosate herbicide analysis by post-column HPLC according to US EPA Method 547 employs a cation-exchange column. 

Many polyvalent metal ions which may be present in the sample, especially Iron, will accumulate in the guard or analytical 

column. As little as 100 nmole of Ferric Iron, for example, will cause serious degradation of column performance; 

larger amounts can actually cause the glyphosate peak to vanish completely. 

Glyphosate RESTORE rapidly removes Iron and all other polyvalent metals from the column and guard. In addition, it 

preserves the balance of K + /H + in the resin, thus avoiding a lengthy re-equilibration. 

RESTORE CATALOG INFORMATION 


1700-0140 RESTORE, removes metal ions from Glyphosate column and guard each (250 mL/bottle) 

PURE WATER 

PURE WATER CATALOG INFORMATION 


1700-2300 Ultra High Pure Water 4 x 950 mL/bottle 

EXTRACTION SOLUTION 

AMINOGLYCOSIDE EXTRACTION SOLUTION CATALOG INFORMATION 


1750-1118 Aminoglycoside Extraction Solution 4 x 950 mL/bottle 

Li220 & Na220 

CHROMATOGRAPHIC GRADE SAMPLES AND STANDARDS DILUENTS FOR AMINO ACID ANALYSIS 

Use of these diluents is essential to ensuring reproducibility from injection to injection. They establish a uniform pH and ion 

concentration at the outset, regardless of the source and pre-treatment of the sample. The sample is maintained buffered and 

at optimum pH for sample storage and analysis. 

Use Na220 for hydrolysate samples and to dilute amino acid calibration standards for use with Sodium columns and 

buffers. Use Li220 for native amino acid samples and calibration standards for use with Lithium columns and buffers. 

Li220 & Na220 CATALOG INFORMATION 


Li220 Lithium Diluent, pH 2.36 case of 4 (250 mL/bottle) 

Na220 Sodium Diluent, pH 2.20 case of 4 (250 mL/bottle)


CALIBRATION STANDARDS & TEST MIXTURES 

• Quantitative and guaranteed 

• Each lot tested chromatographically 

• Chromatographically-pure starting components 

Pickering’s Amino Acid standards have a reputation worldwide for quality and reliability in all post-column systems and 

methods. Six Amino Acid Standard mixtures are available for a variety of applications. Except for the three-component test 

mixture (1700-0070) the standards are in 5 mL vials, in an appropriate citrate buffer. 

Although they are stored frozen at the factory, Pickering’s calibration standards remain stable when shipped at ambient 

temperatures. Upon receipt, however, it is important to place them into a freezer immediately, and store until ready for use. 

Test Mixtures are the qualitative standards and intended to be used to establish an elution profile and for troubleshooting. 

TEST MIXTURES CATALOG INFORMATION 

CATALOG NO. DESCRIPTION CONSTITUENTS 

1700-0070 Amino Acid Test Mixture Concentration 0.25 µmole/mL in 0.01 NHCl, 1.5 mL L-Cysteic Acid 

L-Threonine 

L-Serine 

1700-0063 Carbamate Test Mixture Concentration 2.5 µg/mL in Methanal, 1.5 mL Aldicarb 

Aldicarb Sulfone 

Aldicarb Sulfoxide 

DMC (4-Bromo-3,5-dimethylphenyl-N-methylcarbamate) 

Carbaryl 

Carbofuran 


Methiocarb 

Methomyl 

1-Naphthol 

Oxamyl 

Propoxur 

1700-0080 Glyphosate Test Mixture Concentration 2.5 µg/mL in water, 1.5 mL Glyphosate 

AMPA (Aminomethyl-phosphonic acid) 

55 

CALIBRATION STANDARDS & CATALOG INFORMATION 


DESCRIPTION 

011006P Native Sample Standard with Norleucine, in 0.2 N Lithium citrate buffer pH 2.36, 5 mL 

012006P Native Sample Standard without Norleucine, in 0.2 N Lithium citrate buffer pH 2.36, 5 mL 

1700-0150 Native Sample Standard for rapid-screen for PKU and MSUD, in 0.2 N Lithium citrate buffer pH 2.36, 5 mL 

012506C Collagen Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL 

012506H Protein Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL 

1700-0155 Oxidized Feed Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL 

1700-0170 

Native Sample Standard without Norleucine & Alpha-Amino-Beta-guanidinopropionic acid, in 0.2 N Lithium citrate 

buffer pH 2.36, 5 mL 

1700-0175 Native Sample Standard, basics, in 0.1 N HCl, 5 mL 

1700-0180 Native Sample Standard, acidics and neutrals, in 0.1 N HCl, 5 mL 



10/11


CALIBRATION STANDARDS FOR AMINO ACID ANALYSIS 

CONSTITUENTS 1700-0180* 1700-0175* 011006P 012006P 1700-0150 012506C 012506H 1700-0155 1700-0170 

Beta-Alanine • • • • 

Alanine • • • • • • • 

D,L-a-Amino-adipic acid • • • • 

Gamma-Amino butyric acid • • • • 

Alpha-Amino-n-butyric acid • • • • 

D,L,b-Amino-i-butyric acid • • • • 

Alpha-Amino-Betaguanidinopropionic 

• • 

acid 

Ammonia • • • • • • • 

Anserine • • • • 

Arginine • • • • • • • 

Asparagine • • • • 

Aspartic acid • • • • • • • 

Carnosine • • • • 

Citrulline • • • • 

Creatinine • • • • 

Cystathionine • • • • 

Cystine • (1.25) • • • • • 

Cysteic acid 

• 

Ethanolamine • • • • 

Glutamic acid • • • • • • • 

Glycine • • • • • • • 

Histidine • • • • • • • 

D,L-Homocystine • • • • 

L,L & allo-Hydroxylysine • • • • • 

4-trans-L-Hydroxyproline • • • • (1.25) • 

Isoleucine • • • • • • • • 

Leucine • • • • • • • • 

Lysine • • • • • • • 

Methionine • • • • • • • 

Methionine-D,L-sulfoxide 

• 

Methionine-D,L-sulfone 

• 

1-Methyl-histidine • • • • 

3-Methyl-histidine • • • • 

Norleucine 

• 

Ornithine • • • • 

Phenylalanine • • • • • • • • 

Phosphoethanolamine • • • • 

Phosphoserine • • • • 

Proline • • • • (1.25) • • • 

Sarcosine • • • • 

Serine • • • • • • • 

Taurine • • • • 

Threonine • • • • • • • 

Tryptophan • • • • • 

Tyrosine • • • • • • • 

Urea • • • • 

Valine • • • • • • • 

NOTE: Concentration of all the constituents in the Amino Acid standards is 0.25 µmole/mL unless otherwise specified. 

*Concentration of all the constituents is 2.5 µmole/mL unless otherwise specified


ARTIFICIAL PERSPIRATION 

FOR TESTING OF PRODUCT COLORFASTNESS, CORROSION, DISCOLORATION 

The artificial Eccrine Sweat we offer is a stabilized, ready to use solution. It consists of the 19 amino acids, the seven most 

abundant minerals and the four most abundant metabolites at a pH of 4.5. All concentrations closely match experimentally 

determined values for adult human Eccrine Sweat. Custom formulations are available on request. 

Store and Handling 

The stabilized solution is preserved with a fungicide and bactericide for one year shelf life. 

The non-stabilized product is kept frozen. 

METABOLITES 

MINERALS 

AMINO ACIDS 

ARTIFICIAL ECCRINE PERSPIRATION LIST OF INGREDIENTS 

Uric Acid 

Urea 

Lactic Acid 

Ammonia 

Sodium Iron Phosphate 

Calcium Copper Sulfate 

Magnesium 

Potassium 

Zinc 

Chloride 

Glycine L-Histidine L-Serine (Largest amount) 

L-Alanine L-Isoleucine L-Threonine 

L-Arginine L-Leucine L-Tyrosine 

L-Asparagine L-Lysine L-Valine 

L-Aspartic acid L-Methionine Taurine 

L-Citrulline 

L-Ornithine 

L-Glutamic acid 

L-Phenylalanine 

57 

ARTIFICIAL PERSPIRATION CATALOG INFORMATION 


1700-0020 Artificial Eccrine Perspiration – stabilized 200 mL 

1700-0022 Artificial Eccrine Perspiration – not stabilized 200 mL 

1700-0024 Artificial Eccrine Perspiration – stabilized 5 mL* 

*Ask for a free sample 

NOTE: Custom pH formulations can be made in the pH range 3.0 to 9.0. 

For custom component formulations please contact support@pickeringlabs.com for quote. 



10/11

ELUANTS 


Pickering Laboratories eluants are manufactured under 

strictly controlled conditions to guarantee purity, stability 

and consistency for a reproducible high quality chromatogram. 

This quality standard guarantees the resulting chromatogram 

will be free of any noise and interference. 

The Lithium and Sodium eluants are not sensitive to oxidation 

and do not need refrigeration, either in storage or use. 

Degassing is not required. However, they should be protected 

from air to prevent contamination. Ambient air actually 

contains amines and amino acids that will dissolve in the 

low-pH eluants and will appear in the chromatograms. 

All buffers are packaged in cases of four 950 mL polyethylene 

bottles. The regenerants are packaged in single 950 

mL bottles because of the small volumes used during each 

analysis. Sample diluents are packaged in cases of four 250 

mL borosilicate glass bottles. 

CUSTOM ELUANTS 

We develop and manufacture custom lots of Eluants for 

customers who require our “Guaranteed Chemistry” commitment 

of purity and certified use. Let us make some for 

you! Call for details. 

ELUANTS CATALOG INFORMATION 


Amino Acid Analysis, Sodium-Based Solutions: 

Na270 Sodium Eluant, pH 2.70 case of 4 (950 mL/bottle) 






1700-0112* Sodium Eluant, pH 3.15, 

case of 4 (950 mL/bottle) 

with 5 % Sulfolane, 

1700-0114* Sodium Eluant, pH 3.33, 


with 2.5 % Sulfolane, 

RG011 Sodium Column Regenerant each (950 mL/bottle) 

Na220 Sodium Diluent, pH 2.20, for calibration 


standards/hydrolyzed samples 

Amino Acid Analysis, Lithium-Based Solutions: 

Li275 Lithium Eluant, pH 2.75 case of 4 (950 mL/bottle) 



1700-1125 Lithium Eluant, pH 2.80 case of 4 (950 mL/bottle) 





RG003 Lithium Column Regenerant each (950 mL/bottle) 

Li220 Lithium Diluent, pH 2.36, for calibration 


standards/prepared samples 

Glyphosate Herbicide Analysis: 

K200 Glyphosate Potassium 


Phosphate Eluant 

RG019 Glyphosate Column Regenerant each (950 mL/bottle) 

Potassium-Based Solutions for ALKION Column for Aminoglycoside 

Antibiotics, Paraquat/Diquat in Water Analysis & Streptomycin: 

1700-1101 Potassium Phosphate Eluant, KO1 case of 4 (950 mL/bottle) 

1700-1102 Potassium Hydroxide Eluant, KO2 case of 4 (950 mL/bottle) 

1700-1103 Potassium Chloride Eluant, KO3 case of 4 (950 mL/bottle) 

Biogenic Amines/Polyamines Analysis: 

K563 Potassium Phosphate Eluant, pH 5.63 case of 4 (950 mL/bottle) 

K600 Potassium Phosphate Eluant, pH 6.00 case of 4 (950 mL/bottle) 

K130 Potassium Column Regenerant each (950 mL/bottle) 

Muti-Residue Mycotoxin Analysis: 

1700-1108 Sodium Phosphate Eluant, pH 3.3 case of 4 (950 mL/bottle) 

* For use with 1154150 column with serial numbers after 1314


AMINO ACID ANALYSIS KITS 

AMINO ACID ANALYSIS KITS 


DESCRIPTION 

0352-0061 30-MIN HIGH-EFFICIENCY COLLAGEN HYDROLYSATE KIT: 

1154110T 

30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700- 

0070 amino acid test mixture 

1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDs 

Na220 Sodium Diluent, pH 2.20, 4 x 250 mL 

Na315 Sodium Eluant, pH 3.15, case of 4 ( 950 mL/bottle) 

Na740 Sodium Eluant, pH 7.40, 4 x 950 mL 

RG011 Sodium Column Regenerant, 950 mL 

T100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life) 

012506C Sodium Calibration Standard, for collagen hydrolysate, 0.25 µmole/mL, 5 mL 

0352-0062 KIT IDENTICAL TO 0352-0061 WITH T200 REPLACING T100C: 

T200 

TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL 

(12-month shelf life) 

0352-0063 

KIT IDENTICAL TO 0352-0061 WITH ITEMS BELOW 

REPLACING T100C: 

OD104 OPA Diluent, 4 x 950 mL 

O120 o-Phthalaldehyde, 5 g 

3700-2000 Thiofluor, 10 g (2 each per kit) 

0352-0057 30-MIN HIGH-EFFICIENCY PROTEIN HYDROLYSATE KIT: 

1154110T 

30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700- 

0070 amino acid test mixture 



Na315 Sodium Eluant, pH 3.15, case of 4 ( 950 mL/bottle) 




012506H Sodium Calibration Standard, for protein hydrolysate, 0.25 µmole/mL, 5 mL 


T200 



0352-0059 KIT IDENTICAL TO 0352-0057 WITH ITEMS BELOW REPLACING T100C: 

OD104 OPA Diluent, 4 x950 mL 



AT30SH STANDARD 60-MIN PROTEIN HYDROLYSATE KIT: 

1193250 Sodium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070) 








0352-0030 KIT IDENTICAL TO AT30SH WITH T200 REPLACING T100C: 

T200 



AO30SH KIT IDENTICAL TO AT30SH WITH ITEMS BELOW REPLACING T100C: 

OD104 OPA Diluent, 4 x950 mL 



AT31FH HIGH-EFFICIENCY 55-MIN PROTEIN HYDROYSIS KIT: 

1154150T 

Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 

1700-0070) 








0352-0031 KIT IDENTICAL TO AT31FH WITH T200 REPLACING T100C: 

T200 



AO31FH KIT IDENTICAL TO AT31FH WITH ITEMS BELOW REPLACING A T100C: 




AT32FC HIGH-EFFICIENCY 55-MIN COLLAGEN HYDROLYSATE KIT: 

1154150T 

Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 

1700-0070) 







012506C 

Sodium Calibration Standard, for collagen hydrolysate 0.25 µmole/ 

mL, Proline and Hydroxyproline, 1.25 µmole/mL, 5 mL 

0352-0032 KIT IDENTICAL TO AT32FC WITH T200 REPLACING T100C: 

T200 



AO32FC KIT IDENTICAL TO AT32FC WITH ITEMS BELOW REPLACING T100C: 




0352-0018 60-MIN OXIDIZED FEED HYDROLYSATE KIT: 

1154150T Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070) 







1700-0155 

Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25 

µmole/mL, 5 mL 


T200 



0352-0019 KIT IDENTICAL TO 0352-0018 W/ ITEMS BELOW REPLACING T100C 



3700-2000 Thiofluor, 10 g, (2 each per kit) 

59 



10/11


Amino Acid Analysis Kits Continued 

0352-0006 

70-MIN HIGH-EFFICIENCY PHYSIOLOGIC 

FLUID/NATIVE SAMPLE KIT: 

0354675T Lithium Ion-exchange Column, 4.6 x 75 mm (with test mixture 1700-0170) 


1700-1125 Lithium Eluant, pH 2.80, 4 x 950 mL 

Li220 Lithium Diluent, pH 2.20, 4 x 250 mL 



RG003 Lithium Column Regenerant, 950 mL 

SP100 SERAPREP, 250 mL 

UP100 URIPREP, 250 mL 



T200 



0352-0008 






AT33SP STANDARD 185-MIN PHYSIOLOGIC FLUID/NATIVE SAMPLE KIT: 

0393250 Lithium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070) 


Li275 Lithium Eluant, pH 2.75, 4 x 950 mL 





011006P Lithium Calibration Standard, with norleucine, 0.25 µmole/mL, 5 mL 



0352-0033 KIT IDENTICAL TO AT33SP WITH T200 REPLACING T100C: 

T200 



AO33SP KIT IDENTICAL TO AT33SP WITH ITEMS BELOW REPLACING T100C: 




0352-0015 

120-MIN HIGH-EFFICIENCY PHYSIOLOGIC 


0354100T 

Lithium Ion-exchange Column, 4.0 x 100 mm (with test mixture 

1700-0070) 







011006P Lithium Calibration Standard, with norleucine, 0.25 µmole/mL, 5 mL 




T200 



0352-0012 





3700-2000 Thiofluor, 10 g ( 2 each per kit) 

0352-0013 

TEMPERATURE GRADIENT 90 MIN PHYSIOLOGIC 


0354100T 

Lithium Ion-exchange Column, 4.0 x 100 mm (with test mixture 

1700-0170) 


1700-1125 Lithium Eluant, pH 2.80, 4 x 950 mL 









T200 



0352-0016 

KIT IDENTICAL TO 0352-0013 WITH ITEMS 

BELOW REPLACING T100C: 




AT35PK 

HIGH-EFFICIENCY 7-MIN PKU 

DETECTION/PHYSIOLOGIC FLUID KIT: 

0354050 Lithium Ion-exchange Column, 4.0 x 50 mm 






1700-0150 

Calibration Standard, contains Ile, Leu, Met, Tyr, & Phe, 0.25 µmole/ 

mL, 5 mL 

0352-0035 KIT IDENTICAL TO AT35PK WITH T200 REPLACING T100C: 

T200 


(12-month shelf life)


PESTICIDE & HERBICIDE ANALYSIS KITS 

PESTICIDE & HERBICIDE ANALYSIS KITS 


DESCRIPTION 

0352-0002 CARBAMATE PESTICIDES ANALYSIS KIT, 23+ COMPOUNDS: 

0840250 

Analytical Column, C 8 

, expanded resolution, 4.0 × 250 mm (with 

Carbamate Test Mixture 1700-0063) 


CB130 Carbamate Hydrolysis Reagent, 4 × 950 mL 

CB910 Carbamate OPA Diluent, 4 × 950 mL 



1700-0132 ChlorAC Buffer, 250 mL 

0352-0003 CARBAMATE PESTICIDES ANALYSIS KIT, FOR EPA METHOD 531.1: 

1846250 


, high resolution/capacity, 4.6 × 250 mm 

(with Carbamate Test Mixture 1700-0063) 







0352-0009 CARBAMATE PESTICIDES ANALYSIS KIT, FOR EPA METHOD 531.2: 

1846250 


, high resolution/capacity, 4.6 × 250 mm 

(with Carbamate Test Mixture 1700-0063) 


CB130.2 Carbamate Hydrolysis Reagent, 4 × 950 mL 





0352-0004 

CARBAMATE PESTICIDES ANALYSIS KIT, FOR AOAC 

METHOD 985.23: 

1846150 

Analytical Column, C18, rapid analysis/QC, 4.6 × 150 mm (with 

Carbamate Test Mixture 1700-0063) 







0352-0010 

GLYPHOSATE HERBICIDE ANALYSIS KIT, FOR EPA METHOD 

547, AOAC METHOD 991.08: 

1954150 

Cation-exchange Column, K form, 4.0 × 150 mm (with Glyphosate 

Test Mixture 1700-0080) 


K200 Glyphosate Potassium Phosphate Eluant, 4 × 950 mL 

RG019 Glyphosate Column Regenerant, 950 mL 

GA104 Glyphosate OPA Diluent, 4 × 950 mL 

GA116 Glyphosate Hypochlorite Diluent, 4 × 950 mL 



1700-0140 RESTORE, 250 mL 

OTHER POST-COLUMN ANALYSIS KITS 

OTHER POST-COLUMN ANALYSIS KITS 


DESCRIPTION 

0352-0040 BIOGENIC AMINES/POLYAMINES ANALYSIS KIT: 

9410917 ALKION Cation-exchange Column, K form, 4.0 × 150 mm 

9493020 ALKION Guard Column, K form, 3.0 × 20 mm 

1100-0200 Flow Restrictor, 300 psi, 10 cm 

K563 Potassium Phosphate Eluant, pH 5.63, 4 × 950 mL 

K600 Potassium Phosphate Eluant, pH 6.00, 4 × 950 mL 

K130 Potassium Column Regenerant, 950 mL 

OD104 OPA Diluent, 4 × 950 mL 



0352-0041 AMINOGLYCOSIDE ANTIBIOTICS ANALYSIS KIT: 




1700-1101 Potassium Phosphate Eluant, KO1, 4 × 950 mL 

1700-1102 Potassium Hydroxide Eluant, KO2, 4 × 950 mL 

1700-1103 Potassium Chloride Eluant, KO3, 4 × 950 mL 




1700-1118 Aminoglyside extraction solution 4 x 950 mL 

0352-0042 PARAQUAT/DIQUAT IN WATER ANALYSIS KIT: 




1700-1101 Potassium Phosphate Eluant, KO1, 4 × 950 mL 

1700-1102 Potassium Hydroxide Eluant, KO2, 4 × 950 mL 

1700-1103 Potassium Chloride Eluant, KO3, 4 × 950 mL 

0352-0053 MULTI-RESIDUE MYCOTOXIN KIT: 

1612124 MYCOTOX Reversed-phase Column, C 18 

, 4.6 × 250 mm 




1700-1108 Sodium Phosphate Eluant, pH 3.3 


0352-0050 AFLATOXINS ANALYSIS KIT: 

1612124 MYCOTOX Reversed-phase Column, C 18 

, 4.6 × 250 mm 


0352-0051 POLYETHER ANTIBIOTICS KIT: 

2381750 Polyether Column, C 18 

, 4.6 × 250 mm 


3700-2200 Vanillin, 30 g 

0352-0052 PARALYTIC SHELLFISH TOXINS KIT: 

0846150 P.S.P. column, C 8 

, 4.0 × 150 mm 


61 



10/11

BECKMAN 6300/7300 COLUMNS & CHEMICALS 

ANALYTICAL COLUMNS & GUARDS CATALOG INFORMATION 


DESCRIPTION 

0354100T Lithium Cation-exchange column, 4.0 × 100 mm 

1154150B Sodium Cation-exchange column, 4.0 × 150 mm 

0352020 Lithium guard column, 2.0 × 20 mm 

1193020 Sodium guard column, 3.0 × 20 mm 

ELUANTS CATALOG INFORMATION 


Physiological Fluids And Native Samples, 3-Buffer Method: 

Li292 1 Lithium Eluant, pH 2.92 case of 4 (950 mL/bottle) 

1700-1125 2 Lithium Eluant, pH 2.80 case of 4 (950 mL/bottle) 



RG003 Lithium Column Regenerant each (950 mL/bottle) 

Protein Hydrolysate Samples, Classic 3-Buffer Method: 

Na328 3 Sodium Eluant, pH 3.28 case of 4 (950 mL/bottle) 

1700-0114 4 Sodium Eluant, pH 3.33 case of 4 (950 mL/bottle) 



RG011 Sodium Column Regenerant each (950 mL/bottle) 

1 To use with columns 0354100A 

2 To use with columns 0354100T 

3 For columns 1154150 with serial number 1313 and before 

4 For columns 1154150 with serial number 1314 and after 

NOTE: Please contact Pickering to obtain information on 3-buffer method for Collagen 

Hydrolysate Oxidized Feed samples, 5-buffer method for Physiological Fluids and Native 

Samples and for Beckman System Gold 

REAGENTS & CHEMICALS CATALOG INFORMATION 


T100 TRIONE® Ninhydrin Reagent 

each (950 mL/bottle) 

(3-month* shelf life) 

T100C TRIONE® Ninhydrin Reagent 


(3-month* shelf life) 

T200 TRIONE® Two-part Ninhydrin Reagent 

(12-month* shelf life before mixing) 

to prepare case of 4 


Li220 Lithium Diluent, pH 2.36 case of 4 (250 mL/bottle) 

Na220 Sodium Diluent, pH 2.20 case of 4 (250 mL/bottle) 

SP100 SERAPREP each (250 mL/bottle) 

UP100 URIPREP each (250 mL/bottle) 

012006P Native Sample standard, in 0.2 N Lithium each (5 mL/bottle) 

citrate buffer pH 2.36 1 

011006P Native Sample standard, in 0.2 N Lithium each (5 mL/bottle) 

citrate buffer pH 2.36 2 

012506H Protein Hydrolysate standard, in 0.27 N each (5 mL/bottle) 

Sodium citrate buffer pH 2.20 3 

012506C Collagen Hydrolysate standard, in 0.27 N each (5 mL/bottle) 

Sodium citrate buffer pH 2.20 4 

1700-0155 Oxidized Feed Hydrolysate standard, in each (5 mL/bottle) 

0.27 N Sodium citrate buffer pH 2.20 5 

1700-0070 Amino Acid test mixture, in 0.01 N HCl 6 each (1.5 mL/bottle) 

1700-0170 Native Sample Standard in 0.2 N Lithium each (5 mL/bottle) 

citrate buffer, pH 2.36 7 

1700-0175 Native Sample Standard, basics, in 0.1 N each (5 mL/bottle) 

HCl, 5 mL 8 

1700-0180 Native Sample Standard, acidics and each (5 mL/bottle) 

neutrals, in 0.1 N HCl, 5 mL 8 

* From date of manufacture 

1 Without Norleucine, 0.25 µmole/mL 

2 With Norleucine, 0.25 µmole/mL 

3 0.25 µmole/mL 

4 0.25 µmole/mL, Proline & Hydroxyproline, 1.25 µmole/mL 



7 Without Norleucine & Alpha-Amino-Beta -guanidinopropionic acid, 0.25 µmole/mL 

8 2.5 µmole/mL

REPLACEMENT COMPONENTS, FITTINGS & TUBING 

PINNACLE PCX FLOW DIAGRAM– TWO-PUMP SYSTEM 

Nut: 1452-0118 

Ferrule: 1452-0117 

COLUMN 

TRANSDUCER 2 

1452-0184 

1452-0041 

R2 

FLUSH 

R1 

Nut: 1452-0180 

Ferrule: 1452-0181 

1452-0189 

2 

1 

6 

1452-0188 

2103-0463 

PUMP 2 

7130-0008 

3102-9040 

1452-0185 

3 

VALVE 2 

4 

5 

2103-0463 

1452-0041 

FILTER 

1452-0182 

Nut: 1452-0118 

Ferrule: 1452-0117 

HEATED 

REACTOR 

AMBIENT 

REACTOR 

1100-2927 

63 

2102-0463 

Nut: 1452-0118 

Ferrule: 1452-0117 

TRANSDUCER 1 

1452-0040 

1452-0041 

1452-0188 

1452-0189 

Nut: 1452-0114 

Ferrule: 1452-0112 

3102-9040 

Coupler: 3102-3064 

FILTER 

1452-0184 

2 

1 

6 

2102-0463 

PUMP 1 

3 

7130-0008 

VALVE 1 

5 

Nut: 1452-0113 

Ferrule: 1452-0115 

4 

Check Valve: 3106-1007 

Nut: 1452-0226 

Ferrule: 1452-0112 

FROM 

HPLC 

WASTE 

1452-0185 

TO DETECTOR 

Nut: 1452-0118 

Ferrule: 1452-0117 

Union: 3102-3063 



10/11


PINNACLE PCX FLOW DIAGRAM– ONE-PUMP SYSTEM 

Nut: 1452-0118 

Ferrule: 1452-0117 

R1 

Nut: 1452-0180 

Ferrule: 1452-0181 

FLUSH 

1452-0182 

Nut: 1452-0118 

Ferrule: 1452-0117 

2103-0463 

1452-0040 

COLUMN 

FILTER 3102-9040 

2102-0463 

HEATED 

REACTOR 

1452-0187 

1552-0017 

Coupler: 3102-3064 

Nut: 1452-0116 

Ferrule: 1452-0115 

TRANSDUCER 

Nut: 1452-0118 

Ferrule: 1452-0117 

PUMP 

1452-0184 

1 

2 6 

1452-0093 

3 VALVE 1 

4 

5 

1452-0190 

Nut: 1452-0114 

Ferrule: 1452-0112 

Nut: 1452-0113 

Ferrule: 1452-0115 

Nut: 1452-0118 

Ferrule: 1452-0117 

Nut: 3101-0011 

Ferrule: 1452-0112 

Check Valve: 3106-1007 

Nut: 1452-0226 

Ferrule: 1452-0112 

FROM 

HPLC 

1452-0185 

WASTE 

TO DETECTOR 

2102-0463 

Nut: 1452-0118 

Ferrule: 1452-0117 

Union: 3102-3063 

Back Pressure 

Regulator: 3102-9025 

bold = kit


VECTOR PCX FLOW DIAGRAM 

from 

HPLC Column 

HEATED 

REACTOR 

2104-0210 

(PEEK Tubing) 

1452-0040 

(Liquid Manifold) 

3102-9040 

(Filter Frit) 

500 psi Over 

Pressure 

Relief Valve 

A 

A 

3102-9040 

(Filter Frit) 

1452-0041 

(Filter Manifold) 

RESTRICTOR 

B 

RESTRICTOR 

AMBIENT 

REACTOR 

1100-2927 

PULSE 

DAMPENER 

3106-1256 

(Pulse Dampener 

Rebuild Kit) 

PULSE 

DAMPENER 

to 

Detector 

A 

65 

to Injector 

C 

3551-0070 

(Pressure Switch) 

500 psi 

B 

PUMP 1 

3106-1254 

3106-1255 

3106-1257 

3106-1259 

(Vector Pump 

Components) 

PUMP 2 

from 

Pump 

D E F 

C 

D E F 

2103-0463 

Reagent Reservoir & Cap Assemblies 

BOTTLE 

BOTTLE 

A 

Nut: 1452-0118 

Ferrule: 1452-0117 

C 

Nut: 1452-0177 

Ferrule: 1452-0176 

E 

Nut: 1452-0118 

Ferrule: 1452-0117 

B 

Nut: 1452-0179 

Ferrule: 1452-0178 

D 

Nut: 1452-0180 

Ferrule: 1452-0181 

F 

3106-1010 



10/11


PCX 5200 FLOW DIAGRAM 

Injector Pre-column 3102-9042 

Filter (0.5 m) 

Thermostatted 

Column and Guard 

Heated 

Reactor 

Ambient Reactor 

1100-2927 

A 

A 

500 psi 

Pressure Switch 

1552-0002 

3102-3031 

A 

A 

Detector 

3102-3031 

LC Pump 

1452-0035 

Over-pressure relief 

valve (525 psi) 

3102-9013 

Post-column 

Pressure 

1452-0032 

Back-pressure 

Regulator (100 psi) 

3102-9025 

LC Eluants 

Anti-siphon Valves 

3102-9016 

Restrictors 

Waste 

Reagent Filters 

3102-9040 

A 

Bypass Valves 

3104-0080 

Reagent Pressure 

1452-0033 

Reagent 

Pump 

2 

Waste 

Nut: 1452-0180 

Ferrule: 1452-0181 

Check Valve 

3106-1010 

Reagent Bottles 

& Cap Assemblies 

A Nut: 1452-0118 

Ferrule: 1452-0117


PINNACLE PCX REAGENT PUMP 

AND VALVE COMPONENTS 


DESCRIPTION 

1452-0122 Pinnacle PCX Pump Seal Kit 

1352-0007 Pinnacle PCX Pump Cylinder, Ceramic 70 mL 

1452-0038 Pinnacle PCX Pump Assembly, 70 mL with motor 

1452-0093 Pinnacle PCX Valve Face Assembly, liquid end 

1452-0045 Pinnacle PCX Valve Assembly, Valve Face and motor 

1552-0017 Pinnacle PCX Pressure Tranducer Assembly 

1452-0202 Pinnacle PCX Valve Maintenance Kit (Seals and Tools) 

1452-0200 Pinnacle PCX Valve Seal Tool 

1452-0201 Pinnacle PCX Valve Seals Kit 

1100-0500 Pinnacle PCX Tubing Kit - Dual Pump System 

1100-0501 Pinnacle PCX Tubing Kit - Single Pump System 

VECTOR PCX REAGENT PUMP COMPONENTS 


DESCRIPTION 

3106-1254 Pump Check Valve Kit – PEEK (Inlet & Outlet) 

3106-1255 Seal Kit (Piston seal, Back-up O-ring, Diaphragm, Tool) 

3106-1256 Pulse Damper Rebuild Kit (Diaphragm, O-rings, Diaphragm seal, Seal 

tool, 4 Hex wrenches) 

3106-1258 Prime/Purge Valve – PEEK 

3106-1259 Head Kit, Vector Pump, (head, check valves, piston and seal) 

3106-1257 Piston, Vector Pump 

1452-0176 Ferrule, Peek 1/16 x 10-32 S/T, 5 each 

1452-0177 Nut, Peek Long 1/16 x 10-32 S/T, 5 each 

3106-1260 Prime Purge Seal Kit – PEEK 

PCX5200 REAGENT PUMPS 

WITH PISTON WASH 


DESCRIPTION 

1452-0012 Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V 

1452-0013 Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 V 

1452-0010 Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V 

1452-0011 Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 V 

1452-0016 Inert PEEK, Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V 

1452-0017 Inert PEEK, Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 V 

1452-0014 Inert PEEK, Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V 

1452-0015 Inert PEEK, Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 V 

NOTE: 240 V pumps CE certifi ed. Please contact Pickering for pumps without Piston Wash 

PCX5200 REAGENT PUMP COMPONENTS 


DESCRIPTION 

3106-1330 Seal & Backup Ring 

3106-1310 Seal Kit, includes 2 seals, back-up rings, installation tool & hex wrench 

3106-1314 Inlet Check Valve Assembly 

3106-1316 Outlet Check Valve Assembly 

3106-1318 Check Valve Cartridge, inlet or outlet 

3106-1332 Sapphire Piston, for piston-wash 

3106-1333 O-ring, for piston-wash 

3106-5174 Compression Ring 

3106-1322 Piston Guide/Retainer 

3106-1324 Liquid End Assembly 

3106-1326 Preventive Maintenance Kit, includes 2 check-valve cartridge repair 

kits & 3106-1310 items 

3106-1335 Seal Kit PEEK pump 

3106-1336 Check Valve Rebuild Kit 

3106-1337 Check Valve, Inlet PEEK 

3106-1338 Check Valve, Outlet PEEK 

3106-1339 Liquid End Assy, PEEK 

1100-4001 Piston-Wash Upgrade Kit 

3106-1313 Washer, Piston Seal Backup 

3106-1320 Piston, Sapphire no piston-wash 

3106-1342 Spring, Pump Piston Wash 

NOTE: Please contact Pickering for pumps without Piston Wash 

PINNACLE PCX REPLACEMENT 

REACTOR CARTRIDGE 


DESCRIPTION 

1452-0094 Reactor Cartridge Coil Assembly - 0.15 mL 130 °C 



1452-0096 Reactor Cartridge Knitted Assembly - 1.4 mL 130 °C 




1452-0100 Reactor Cartridge Knitted Assembly - 1.2 mL & 1.6 mL 130 °C 

67 



10/11


VECTOR PCX SUBSTITUTE REACTOR 

VOLUMES OR REPLACEMENT REACTORS 


DESCRIPTION 

1452-0162 

1452-0163 

1452-0164 

1452-0165 

1452-0166 

1452-0167 

1452-0174 

1452-0175 

1100-2927 OPA Ambient Reactor, 0.010 in ID TFE Tubing 

1100-0282 Coil Assembly, 0.15 mL without Electronics 



NOTE: Custom volumes are available 

PCX5200 SUBSTITUTE REACTOR VOLUMES 

OR REPLACEMENT REACTORS 


1100-3403 

1100-3404 

1100-3401 

1100-3402 

1100-2654 

1100-2653 

1100-2660 

1100-2661 

1100-3411 

1100-3412 

1100-2659 

1100-2658 

1100-3511 

1100-3512 

1100-2683 

1100-2684 

1100-2681 

1100-2682 

1100-3514 

1100-3513 

Reactor Heater & Coil Assembly, 0.5 mL 150 °C max, 120 V 




Reactor Heater & Knitted Assembly, 1.4 mL 150 °C max, 120 V 

Reactor Heater & Knitted Assembly, 1.4 mL 150 °C max, 240 V 

Reactor Heater & Knitted Assembly, 1.2 & 1.6 mL 150 °C max, 120 V 

Reactor Heater & Knitted Assembly, 1.2 & 1.6 mL 150 °C max, 240 V 

DESCRIPTION 

Reactor Heater & Coil Assembly, 0.1 mL, 150 °C max, 120 V 










Reactor Heater & Knitted Assembly, 1.4 mL, 150 °C max, 120 V 








Reactor Heater & Dual-knitted Assembly, 1.2 & 1.6 mL, 150 °C max, 120 V 

Reactor Heater & Dual-knitted Assembly, 1.2 & 1.6 mL, 150 °C max, 240 V 

1100-2927 OPA Ambient Reactor, 0.010 in. ID TFE Tubing 




1100-0285 Coil Assembly 0.1 mL 

PANELS FOR PCX5200 


DESCRIPTION 

1452-0023 Single-reagent Post-column Gauge Panel 

1452-0020 Dual-reagent Post-column Gauge Panel 

1452-0027 Inert PEEK, Single-reagent Post-column Gauge Panel 

1452-0028 Inert PEEK, Dual-reagent Post-column Gauge Panel 

1452-0021 Reagent 1 Panel, for OPA, Hypochlorite & Hydrolysis Reagent 

1452-0022 Reagent 2 Panel, for OPA Reagent 

1452-0024 Reagent Panel, TRIONE® Ninhydrin Reagent 

1452-0025 Reagent 1 Panel, Inert PEEK flowpath 

1452-0026 Reagent 2 Panel, Inert PEEK flowpath 

REAGENT RESERVOIRS & CAP ASSEMBLIES 


DESCRIPTION 

3107-0137 Reservoir Bottle, safety-coated, 1L 



3107-0300 Reservoir Assembly, includes 1L bottle, and cap with integrated valve 

3107-0147 Cap Assembly for 1L bottle, includes cap with integrated valve 

3107-0148 Cap Assembly for 2L bottle, includes cap with integrated valve 

3107-0138 Reservoir Cap 

1925-0129 Cap for Flush Bottle GL-38, Black, Pinnacle PCX 

1925-0130 Cap for Wash Bottle GL-38, Black, Vector PCX & Pinnacle PCX 

1452-0120 Bottle, Piston Wash with cap, Pinnacle PCX & Vector PCX 

1452-0121 Bottle, System Flush with Cap, Pinnacle PCX 

NOTE: Tubing, nuts and ferrules sold separately 

PRESSURE REGULATORS, 

VALVES & GAUGES 


DESCRIPTION 

1152-0010 Gas Manifold & 5 psi Regulator Assembly, PCX 5200 

1552-0002 Pressure Transducer Assembly, PCX 5200 

3102-9025 Pressure Regulator, 100 psi, 1/4-28 connection, for detector waste, 

PCX 5200, Vector PCX and Pinnacle PCX 

3102-9013 Pressure Relief Valve, 525 psi, PCX 5200 

3102-9016 Reagent Anti-siphon Valve, 60 psi, PCX 5200 

3104-0080 Prime/Purge Valve Assembly, PCX 5200 

3104-0081 Seal Kit for prime/purge valve, PCX 5200 

1452-0032 Pressure Gauge Assembly, 0–1000 psi, PCX 5200 

1452-0033 Pressure Gauge Assembly, 0–3000 psi, PCX 5200 

1452-0034 Pressure Gauge Assembly, 0–1000 psi, for PEEK, PCX 5200 

3102-9015 Back Pressure Regulator, 40 psi Non-Metal, PCX 5200 

1452-0141 Gas Manifold Assembly, Vector PCX and Pinnacle PCX 

3106-1007 Inline Check Valve, Pinnacle PCX 

3106-1010 Check Valve, Reagent Cap


TEES & UNIONS 

FLOW RESTRICTORS 


FILTERS & FRITS 


DESCRIPTION 

1100-0200 Flow Restrictor, for OPA, Hypochlorite & Hydrolysis reagent, 300 psi 

@ 0.3 mL/min, 10 cm 

1100-0141 Flow Restrictor, TRIONE® Ninhydrin reagent, 65 psi @ 0.3 mL/min, 6 cm 

1100-0143 Inert PEEK Flow Restrictor, 550 psi @ 0.3 mL/min, 10 cm 

1100-0142 Flow Restrictor, 100 psi @ 0.3 mL/min, 10 cm 




1100-0300 Static Mixer, internal volume 800 µL 

NOTE: Listed pressures are for a fl ow rate of 0.3 mL/min at ambient temperature 

with 100 % water and pulse-dampened fl ow. 

DESCRIPTION 

3102-9032 Pre-column Filter Assembly, PCX 5200 

3102-9042 Replacement Frit 0.5 µm for 3102-9032 

3102-9036 Reagent Filter Assembly, PCX 5200 

3102-9040 Replacement Reagent 10 µm Frit 

3102-9132 Filter Frit, 2 µm Reagent Filter 

3102-9047 Filter Frit, 0.5 µm PEEK 

3102-9030 In-Line Filter 


DESCRIPTION 

3102-3031 Tee, Valco type, 0.010 holes, 1/16" 

1352-0004 Tee Mount 

3102-4040 Adapter, 1/4 FPT, 1/16" 

3102-3050 Union, 0.020 holes, 1/16" 

3102-3051 Union, 0.050 holes, 1/16" 

3102-3053 Union, Bulkhead 1/4" x 28 

3102-3054 Union, Bulkhead 1/16" x 0.010 

3102-3036 Tee, Peek, 1/16" 

3102-3044 Cross, PEEK 1/16" x 0.010" 

1452-0040 Liquid Manifold with relief Valve Assembly, Vector PCX & Pinnacle PCX 

1452-0041 Liquid “T” Manifold Assembly, Vector PCX & Pinnacle PCX 

TUBING, PLASTIC 


DESCRIPTION 

2101-0006 TFE (Teflon) Tubing, 1/16” OD × 0.006” ID, 90 cm 

2101-0212 TFE (Teflon) Tubing, 1/16" OD × 0.010" ID, 90 cm 

2101-0216 TFE Tubing, 1/16" OD x 0.016" ID, 90 cm 

2101-0220 TFE Tubing, 1/16" OD x 0.020" ID, 90 cm 

2101-0225 TFE (Teflon) Tubing, 1/16" OD × 0.025" ID, 90 cm 

2104-0105 PEEK Tubing, 1/32 x 0.005" ID, 90 cm 

2104-0205 PEEK Tubing, 1/16 OD x 0.005", 90 cm 

2104-0210 Inert PEEK Tubing, 1/16" OD × 0.010" ID, 90 cm 

2104-0220 Inert PEEK Tubing, 1/16" OD × 0.020" ID, 90 cm 

1452-0035 PEEK Tubing Assembly, 0.007" ID × 1/32" (includes nut & ferrules) 

2102-0463 FEP (Teflon clear) Tubing, 1/8" OD × 0.063" ID, 90 cm 

2103-0463 SARAN® Tubing, 1/8" OD × 0.063" ID, 90 cm 

1100-2731 Waste Tube PCX 5200 

2101-0231 C-flex Tubing, 1/8" OD × 1/16" ID, 90 cm 

69 



10/11


TUBING, TYPE 316 STAINLESS STEEL 


DESCRIPTION 

3103-1005 SST Tubing, 1/16" OD × 0.010" ID, 5 cm 







FITTINGS, PLASTIC 


DESCRIPTION 

1452-0180 Nut, 1/4-28, 1/8" (Polypropylene) - Pack of 5 

1452-0181 Ferrule, 1/4-28, 1/8" (Tefzel) - Pack of 5 

3101-0007 Nut, 1/4-28, 1/16" (Kel-F) 

3101-0008 Ferrule, 1/4-28, 1/16" (Tefzel) 

3102-1110 Nut, 1/4 -28, 1/16" (Tefzel) 

3102-2110 Ferrule, 1/4-28, 1/16" (Tefzel) 

3101-0020 Plug (Male), 1/4-28 (Delrin) 

3101-0030 Plug (Male), 10-32, 1/16" (Delrin) 

3101-0060 Nut, Fingertight, 1/16" (Kel-F) 

1452-0118 Nut, 1/16 (PEEK), Lite Touch - Pack of 5 

1452-0117 Ferrule, 1/16 (PEEK), Lite Touch - Pack of 5 

3102-9160 Removal Tool, for Lite-Touch Ferrule 

3102-1302 Nut, Male 1/4-28 1/16 tubing SST 

3102-2108 Ferrule, 1/4" SST 

3101-0011 Nut, Female PEEK 1/4-28 

1452-0112 Ferrule, 1/16 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 5 

1452-0113 Nut, Short, 1/8 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 5 

1452-0114 Nut, Short, 1/16 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 5 

1452-0115 Ferrule, 1/8 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 5 

1452-0116 Nut, Long, 1/8 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 5 

1452-0226 Nut, Long, 1/16 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 5 

FITTINGS, TYPE 316 STAINLESS STEEL 


DESCRIPTION 

1452-0179 Nut (Male), Parker type, 10-32, 1/16" - Pack of 5 

1452-0178 Ferrule, Parker type, 1/16" - Pack of 5 

3102-1402 Nut (Male), Valco type, 10-32, 1/16" 

3102-2402 Ferrule, Valco type, 1 /16" 

3102-1404 Nut, Valco type, 1/32" 

3102-2404 Ferrule, Valco type, 1/32"


FUSES & MISC. ACCESSORIES 


DESCRIPTION 

3543-0045 Fuse, for 120 V instruments, PCX 5200 

3543-0044 Fuse, for 240 V instruments, PCX 5200 

3543-0010 Fuse, for power supply (PCB), PCX 5200 

3543-0014 Fuse, 3.15A Time Delay 

3551-0073 Fuse, 5A Time Lag Highbreak, ceramic, Pinnacle PCX 

3107-0020 Syringe, 20 cc 

3102-9161 Tubing Cutter, for 1/8–1/16 in. tubing 

1100-0791 Plug, 1/16" 10-32 Male SST 

1100-4020 System Connection Harware Kit 

1100-4022 Column Fittings Kit, 1/16" SST 

1452-0029 Heat-Exchanger for Reactor 

3102-3064 Coupler for Guard and Column 

1452-0185 Pinnacle PCX , Tubing Assembly, Valve-Pump (2 each) 

1452-0184 Pinnacle PCX, Tubing Assembly, Pump-Transducer (2 each) 

1452-0183 Pinnacle PCX, Tubing Assembly, Column out – 

Manifold, Dual pump system 

1452-0182 Pinnacle PCX, Tubing Assembly, Column out – 

Manifold, Single pump system 

1452-0186 Pinnacle PCX & Vector PCX, Tubing Assembly, Gas Manifold-Bottle 

1452-0187 Pinnacle PCX, Tubing Assembly, Valve-Manifold, Single pump system 

1452-0188 Pinnacle PCX, Tubing Assembly, Transducer – Valve (2 each) 

1452-0189 Pinnacle PCX, Tubing Assembly, Valve Manifold (2 each) 

Dual pump system 

1452-0190 Pinnacle PCX, Tubing Assembly, Transducer – 

Valve, Single pump system 

0101-0008 Manual, Pinnacle PCX operations 

0101-0009 Manual, Vector PCX operations 

3560-2000 Cable, RS232, 10’ 

1452-0126 Relay Cable Assembly 

1552-0033 Enable, Switch Vector 

71 

The following trademarks are the property of the companies listed: 

ALKION 

Brij-35 

ChlorAC 

Chromatographic Grade 

Hydrolysis Reagent C47 

RESTORE 

Saran 

Seraprep 

Tefzel 

Thiofluor 

TRIONE 

Uriprep 

Vespel 

Windows 


ICI Americas, Inc. 





Dow Chemical Corporation 


E.I. DuPont Corporation 




E.I. DuPont Corporation 

Microsoft Corporation 



10/11

“CUSTOMERS REALLY ONLY WANT THE CHROMATOGRAM. 

WE MAKE SURE THEY GET IT – GUARANTEED.” 

We take responsibly for all components of a method from the column, instrument and reagents. 

These methods are robust, rugged and very analyte selective. 

MARIA OFITSEROVA / SENIOR RESEARCH CHEMIST 

SAREETA NERKAR / RESEARCH CHEMIST 

Although multiuse, HPLC’s are 

very specific when in use.


110 / AFLATOXINS 

TOXINS IN PEANUTS, VEGETABLE MATTER, AND MILK 

Aflatoxins occur naturally in peanuts, peanut meal, cottonseed meal, corn, dried chili pepper, etc. However, the growth 

of mold does not always indicate the presence of toxin since the yield of Aflatoxins is dependent on growth conditions 

such as moisture, temperature, and aeration. The Aflatoxins are characterized as B for blue fluorescence and G for green 

fluorescence. The numerical subscripts indicate relative chromatographic mobility. Besides the toxins commonly found in 

vegetable matter (B1, B2, G1, and G2), Aflatoxins M (for milk) are found in milk of cows fed toxic meals. The highly toxic 

M metabolites are 4-hydroxylated Bs. 

The most important feature of the post-column method described here is that all four Aflatoxins are detectable at the same 

fluorescence emission wavelength in a single run. The Pickering Pinnacle PCX with a 1.4 mL reactor is recommended for 

this method. 

METHOD 


Column: MYCOTOX column, Catalog No. 1612124 



Mobile Phase: MeOH, CH 3 

CN, H 2 

O; 22:22:56, Isocratic 

G1 B2 

B1 


Post-column System: Pinnacle PCX with 1.4 mL reactor 


Reagent: I2 100 mg/L in water 


Detection: Fluorometer, Xenon lamp 

λ ex 

: 365 nm, λ em 

: 430 nm 

73 

M2 

M1 

G2 

REFERENCES 

1) R. Buchi in “Afl atoxins,” L. Goldblat, Ed., Academic Press, 

New York, NY (1969) 

2) C.W. Thorp, G.M. Ware, and A.E. Pohland,“Proceedings of the 5th 

International IUPAC Symposium on Mycotoxins and Phycotoxins,” 

W. Pfannhauser and P.B. Czedic-Eysenberg (Eds.), 

Technical University, Vienna (1982) 52–55 

3) J.W. Dorner & R.J. Cole, J.A.O.A.C., 71 (1988) 43–47 

4) M.J. Shepherd and J. Gilbert, Food Additives Contaminants, 1 

(1984) 325–335 

0 

2 4 

6 

8 10 12 14 

min 



10/11


203.1 / MULTI-RESIDUE MYCOTOXIN ANALYSIS 

SINGLE RUN ANALYSIS OF DEOXYNIVALENOL, AFLATOXINS, OCHRATOXIN A, 

ZEARALENONE AND FUMONISIN BY HPLC AND POST-COLUMN DERIVATIZATION 

Maria Ofitserova, PhD, Sareeta Nerkar, PhD, Michael Pickering, PhD 

Although Aspergillus (Aflatoxins, Ochratoxin A) are generally associated with peanuts and Fusarium (Deoxynivalenol, 

Zearalenone) with wheat, these fungi and those that produce other toxins are not host selective and so can cross plant species. 

This situation is complicated by the fact that the microscopic mold may not be visible to the naked eye. Also, when infected 

grains are processed, any visible mold is lost but the toxic metabolites carry over into the finished products. Thus, multi-residue 

analytical screens for toxins in grain and finished goods are a wiser choice than single-family protocols. We present a single 

screen to cover five families of toxins. This method is suitable for analyzing beverages, grains and feeds. 


25 g of finely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of filtered extract 

is diluted with 70 mL of Phosphate Buffered Saline (PBS). Aflatoxins, Zearealenone and Ochratoxin A are isolated using 

AOZ Immunoaffinity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are eluted 

with 2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoaffinity column (Vicam, USA) according to 

the procedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol. 

To isolate DON 3 mL of filtered extract is mixed with 6 mL of Acetonitrile and cleaned with MycoSep 277 column 

(Romer Labs, USA) according to the manufacture’s instructions. The cleaned solution is filtered and 5.5 mL of it is combined 

with eluants from AOZ and FumoniTest columns. The solution is evaporated to 0.5 mL and final volume is adjusted 

to 1 mL with Methanol. 

METHOD 



, 

4.6x250 mm Catalog No. 1612124 





HPLC PROGRAM 

TIME (Min) 1700-1108 ,% METHANOL, % ACETONITRILE, % 

0.0 85 0 15 

5.0 85 0 15 

5.1 57 28 15 

20.0 57 28 15 

23.0 40 60 0 

40.0 40 60 0 

50.0 20 0 80 

60.0 20 0 80 

REFERENCES: 

Ofi tserova, M., Nerkar, S., Pickering, M., Torma, L., Thiex, N., 

Multiresidue Mycotoxin Analysis in Corn Grain by Column 

High-performance Liquid Chromatography with Post-column 

Photochemical and Chemical Derivatization: Single-Laboratory 

Validation., (2009), J AOAC Int., 92, 15-25 







Detection: 

Fluorescence 


λ ex 

= 365 nm; λ em 

= 455 nm 


λ ex 

= 330 nm; λ em 

= 465 nm 

Ochratoxin A 

λ ex 

= 335 nm; λ em 

= 455 nm 

Zearalenone 

λ ex 

= 275 nm; λ em 

= 455 nm 

UV/Vis 

Deoxynivalenol 

λ=218 nm


FLD Signal 

reagent ON 

reagent OFF 

UV signal 

DON 

FB1 

reagent ON 

FB2 

G1 

G2 B2 

B1 

Ochra 

ZON 

0 10 20 30 40 50 min 

Chromatogram of a standard solution of mycotoxins. Concentrations of toxins (ng/mL): deoxynivalenol (DON) 930, 

aflatoxin B1 4.5, aflatoxin B2 1.6, aflatoxin G1 4.7, aflatoxin G2 2, ochratoxin A 92, zearalenone 481, fumonisin B1 

474, and fumonisin B2 627. 

75 

FLD Signal 

reagent ON 

FB1 

reagent OFF 

UV signal 

reagent ON 

FB2 

DON 

G1 

G2 B2 

B1 

Ochra 

ZON 

0 10 20 30 40 50 min 

Chromatogram of a corn grain sample naturally contaminated with fumonisins FB1 and FB2 and spiked with DON; 

aflatoxins B1, B2, G1, and G2; ochratoxin A; and zearalenone. Concentrations of toxins in the sample (ng/g): 

deoxynivalenol 930, aflatoxin B1 5.0, aflatoxin B2 1.7, aflatoxin G1 5.1, aflatoxin G2 2.2, ochratoxin A 102, 

zearalenone 529, fumonisin B1 1838, and fumonisin B2 1107. 



10/11


208 / MULTI-RESIDUE MYCOTOXIN ANALYSIS OF DRY DISTILLERS GRAINS 

SINGLE RUN ANALYSIS OF AFLATOXINS, OCHRATOXIN A, ZEARALENONE 

AND FUMONISINS BY HPLC AND POST-COLUMN DERIVATIZATION 

Distillers grains (DG) are the still residues after the ethanol has been collected. Approximately 90 % of US production is 

used in domestic animal feed. Any Mycotoxins present in the fresh corn can be concentrated by a factor of three. Contamination 

can also occur during storage. This raises concern about the potential animal and human health hazards from 

the use of Mycotoxin contaminated distillers grains. Corn entering the ethanol processing plant as well as distillers grains 

should be routinely tested for Mycotoxin contaminations to ensure compliance with guidelines set by FDA. 

We present a single screen method to cover 4 families of toxins that could be present in dry distillers grains (DDG). 


25 g of finely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of filtered extract is 

diluted with 70 mL of Phosphate Buffered Saline (PBS). Aflatoxins, Zearealenone and OchratoxinA are isolated using AOZ 

Immunoaffinity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are eluted with 

2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoaffinity column (Vicam, USA) according to the 

procedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol and combined with eluant from 

AOZ column. Solution is evaporated to 0.5 mL and final volume is adjusted to 1 mL with Methanol. 

METHOD 



, 

4.6x250 mm Catalog No. 1612124 











Detection: 

Fluorescence 


λ ex 

= 365 nm; λ em 

= 455 nm 


λ ex 

= 330 nm; λ em 

= 465 nm 

Ochratoxin A 

λ ex 

= 335 nm; λ em 

= 455 nm 

Zearalenone 

λ ex 

= 275 nm; λ em 

= 455 nm 

REFERENCES: 

Ofi tserova, M., Nerkar, S., Pickering, M., Torma, L., Thex, N., Multiresidue Mycotoxin Analysis 

in Corn Grain by Column High-Performance Liquid Chromatography with Post-column 

Photochemical and Chemical Derivatization: Single-Laboratory Validation. 

(2009) J.AOAC Int., 92, 15-25


FB2 

FB1 

Mycotoxin Standard 

OPA on 

OPA off 

OPA on 

Ochra 

G1 

G2 B2 

B1 

Zea 

0 10 20 30 40 50 

min 

MYCOTOXIN 

SPIKE 

CONC, ppb 

NATURAL CONTAMINATION 

LEVEL, ppb 

RECOVERIES % RSD N=4, % 

Aflatoxin B1 10.0 65 7.6 

Aflatoxin B2 3.4 79 6.3 

Aflatoxin G1 10.2 75 9.4 

Aflatoxin G2 4.4 82 9.1 

Ochratoxin A 203 89 7.1 

Zearalenone 1057 231 75 8.8 

Fumonisin B1 1042 801 109 5.8 

Fumonisin B2 1379 223 104 6.8 

77 

DDG Sample Spiked with Mycotoxins 

OPA on 

FB1 

FB2 

OPA off 

OPA on 

G2 

G1 

B2 B1 

Ochra 

Zea 

0 10 20 30 40 50 

min 

5-POINT CALIBRATION CURVES 

MYCOTOXIN CONCENTRATION RANGE CORRELATION 

Aflatoxin B1 0.23 – 113.1 ppb 0.99926 

Aflatoxin B2 0.2 – 39.7 ppb 0.99966 

Aflatoxin G1 0.5 – 58.2 ppb 0.99933 

Aflatoxin G2 0.2 – 24.7 ppb 0.99941 

Ochratoxin A 9.2 – 1155 ppb 0.99926 

Zearalenone 0.024 – 12.01 ppm 0.99908 

Fumonisin FB1 0.024 – 11.84 ppm 0.99987 

Fumonisin FB2 0.031 – 7.84 ppm 0.99993 



10/11


328 / ANALYSIS OF ALENDRONIC ACID IN PHARMACEUTICAL FORMULATIONS 

Alendronic acid or Alendronate Sodium is a bisphosphonate drug that inhibits osteoclast-mediated bone resorption. 

Biphosphates work by binding to the bone and preventing calcium from being removed by osteoclasts. This drug is used 

to prevent and treat osteoporosis as well as several other bone diseases. Alendronate Sodium is sold as tablets or solutions, 

alone or in combination with Vitamin D. 

Since Alendronic acid has no chromophore, post-column derivatization is employed to produce a fluorescence derivative. 

This abstract describes selective and sensitive analytical method for the analysis of Alendronic Acid in pharmaceutical tablets. 

Simple sample preparation and fast analysis time allow for using this method in high throughput environments. 

METHOD 


Column: Anion-exchange, 

4.1x150 mm, 

catalog number 1441150 



Mobile Phase: Sodium Nitrate, 50 mM, 

pH 7.8 / Methanol (60:40) 





Reagent: OPA (300 mg), Thiofluor (2g), 

30 % Brij 35 (3 mL) in 950 mL of OD104 



λ ex 

: 330 nm, λ em 

: 465 nm 

Area 

2000 

1800 

R 2 = 0.9982 

1600 

1400 

1200 

1000 

800 

600 

400 

200 

0 

0 200 400 600 800 1000 1200 

Concentration, ppm 

Calibration curve for analytical range 100-1000 ppm 

Alendronate Sodium 


Crush 1 tablet and mix with water. Use enough water to get 

Alendronate Sodium concentration between 300 and 1000 

μg/mL. Heat the solution using a water bath to 65 ºC and 

stir for 30 min. Filter liquid portion through 0.45 μm filter. 

Put filtrate in HPLC autosampler vial and inject 10 μL. 

0 5 10 

Chromatogram of Fosavance® tablet 

(Alendronate sodium 70 mg, Vitamin D 2800 IU) 

min


380 / 30-MINUTE AMINO ACID ANALYSIS OF HYDROLYZED SAMPLES 

Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. No 

other technique has been shown to match the reproducibility and selectivity of ion-exchange analysis with post-column Ninhydrin 

detection. Nor is there a chromatography technique that provides as much information; the 570/440 nm signal ratio for 

each amino acid is a constant and so offers information about peak purity. 

Pinnacle PCX post-column derivatization system provides a unique opportunity to combine eluant gradient capabilities 

of modern HPLC instruments with column temperature gradients. As might be expected, this capability also reduces the 

analysis time. 

We introduce an accelerated Amino Acid analysis method for hydrolyzed samples that utilizes temperature and 

eluant gradients. 

METHOD 


Column: High-efficiency Sodium 

cation-exchange column, 4.6 x 110 mm, 

Catalog number 1154110T 


Mobile Phase: Na315, Na740, RG011 





TIME TEMP ºC 

0 46 

3 46 

10 55 

22 70 

27 70 

28 46 

HPLC PROGRAM 

TIME Na315 % Na740 % RG011 % 

0 100 0 0 

7.3 100 0 0 

15 53 47 0 

15.1 0 100 0 

28 0 100 0 

28.1 0 0 100 

30 0 0 100 

79 


Reagent: Trione Ninhydrin reagent 


Detection: UV/VIS 570 nm for primary amino acids 

440 nm for secondary amino acids 

Runtime: 30 min 

Equilibration Time: 9 min 

1 23 4 

5 

6 7 8 

12 

9 10 15 

11 

13 

14 

16 

17 

18 

19 

0.0 5.0 10.0 15.0 20.0 25.0 

min 

Chromatogram of Protein Hydrolysate Standard 

1 

2 

3 

4 

5 

6 

7 

Aspartic Acid 

Threonine 

Serine 

Glutamic Acid 

Proline 

Glycine 

Alanine 

8 

9 

10 

11 

12 

13 

14 

Cystine 

Valine 

Methionine 

Isoleucine 

Leucine 

Tyrosine 

Phenylalanine 

15 

16 

17 

18 

19 

Lysine 

Histidine 

Ammonia 

Tryptophan 

Arginine 



10/11


382 / 70-MIN AMINO ACID ANALYSIS OF 

PHYSIOLOGICAL SAMPLES 

Pickering Laboratories specializes in the manufacturing of cation-exchange 

columns and eluants for amino acid analysis. Post-column derivatization 

with Ninhydrin offers unmatched selectivity and reproducibility of the 

analysis for most challenging matrixes. 

Pinnacle PCX post-column derivatization system allows analysts to combine 

eluant gradient capabilities of modern HPLC instruments with column 

temperature gradients. We introduce new accelerated Amino Acids Analysis 

method for physiological samples that utilizes temperature and eluant 

gradients. 


TIME TEMP ºC 

1 

0 34 

6 34 

17 65 

25 70 

70 70 

71 34 

HPLC PROGRAM 

TIME 1700-1125 % Li365 % Li375 % RG003 % 

0 100 0 0 0 

10 100 0 0 0 

19 40 60 0 0 

32 0 100 0 0 

43 0 100 0 0 

43.1 0 0 100 0 

57 0 0 100 0 

57.1 0 0 70 30 

72 0 0 70 30 

72.1 100 0 0 0 

METHOD 


Column: High-efficiency Lithium 

cation-exchange column, 

4.6 x 75 mm, 5 μm 

Catalog number 0354675T 


Mobile Phase: 1700-1125, Li365, Li375, RG003 





Reagent: Trione 


Detection: UV/VIS 570 nm for primary amino acids 

440 nm for secondary amino acids 

Runtime: 72 min 

Equilibration Time: 15 min 

43 

20 

22 

2 

3 

5 

7 8 11 

10 

9 

13 

15 

17 

16 

18 21 

19 

24 

23 

29 

31 

34 

33 

35 

36 

38 

37 

39 

42 

25 26 

27 28 

4 

6 

12 

14 

: 

30 

32 

40 41 

0 10 20 30 40 50 60 70 

min 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

Phosphoserine 

Taurine 


Urea 

Aspartic acid 


Threonine 

Serine 

Asparagine 

Glutamic acid 

Glutamine 

12 Sarcosine 


14 Proline 

15 Glycine 

16 Alanine 

17 Citrulline 


19 Valine 

20 Cystine 

21 Methionine 


23 Isoleucine 

24 Leucine 

25 Tyrosine 


27 β-Alanine 




31 Tryptophan 


33 Ammonia 

34 

35 

36 

37 

38 

39 

40 

41 

42 

43 

Hydroxylysines 

Ornitine 

Lysine 


Histidine 


Anserine 

Carnosine 

Arginine 


*Internal Standard

Met 

Tyr 

Phe 

Leu + Ile 


107 / PKU AND MSUD 

SCREENING FOR PHENYLKETONURIA AND MAPLE SYRUP URINE DISEASE 

Phenylketonuria (PKU) and Maple syrup urine disease (MSUD, branched-chain ketoaciduria) are genetically transmitted 

metabolic diseases. PKU results in elevated Phenylalanine levels due to deficient activity of the enzyme Phenylalanine 

hydroxylase. In the MSUD state, elevated levels of leucine (Leu), isoleucine (Ile), valine (Val), and alloiso-leucine can be 

detected in blood. The increased concentration of the branched-chain amino acids is caused by an inherited deficient activity 

of the enzyme branched-chain keto-acid decarboxylase. 

Failure to diagnose and treat these conditions in newborns can result in severe mental retardation (PKU) or even death (MSUD). 

Dietary restriction of Val, Leu, and Ile for MSUD newborns must begin immediately once the diagnosis is made or suspected. 

MSUD patients must be monitored on a regular schedule in conjunction with the dietary therapy because even 

relatively small changes in the intake of the branched-chain amino acids may produce rather large fluctuations of their concentrations 

in the plasma. In this respect, MSUD is more difficult to control by dietary means than phenylketonuria (PKU), 

because each of the branched-chain amino acids has to be individually adjusted and monitored. 

The Pickering Laboratories PKU/MSUD post-column system is a rapid, automated method for the quantitative analysis of 

Met, Leu + Ile, Tyr and Phe from whole blood samples. Between-injection time is 13 min while actual time is only 7 min. 

The Pickering lithium ion-exchange column has a matched set of eluant and regenerant to provide the best possible separation, 

sensitivity, and reproducibility. The post-column derivatizing reagent is TRIONE® Ninhydrin. 

METHOD 


Column: PKU/MSUD column, 4.0 x 50 mm 

Catalog No. 0354050 



Mobile Phase: Eluant (A): Li357, Regenerant (B): RG003 

Gradient: 

0–8.0 min 100 % A 

8.1–10.0 min 100 % B 

10.1–13.0 min 100 % A 



Reaction Dwell: 0.5 mL 


Reagent: TRIONE® Ninhydrin 


Detection: Visible @ 570 nm 

0 

1 2 

3 

Neonate serum 

with elevated 

Phenylalanine 

Normal 

Neonate serum 

4 5 6 7 8 

min 

This chromatogram is reproduced by courtesy of Quynh Vantu and Wanda Andrews, Virginia 

Newborn Screenng Laboratory, Richmond, Virginia. 

81 

“TRIONE” (U.S. Patent No. 4,274,833) is a registered 

trademark of Pickering Laboratories, Inc. 



10/11


205 / AMINOGLYCOSIDE ANTIBIOTICS IN FEED 

APRAMYCIN, GENTAMICIN, NEOMYCIN 

Aminoglycoside antibiotics are among those commonly used in animal feeds to manage intestinal microorganisms. The 

beneficial effects include improved growth and generally healthier animal populations. Use of antibiotics creates a demand 

for analytical procedures to verify concentrations in pre-mixes and feeds and in some instances for residue analysis in animal 

products. 

This note describes a simple, robust analytical method for the family of Aminoglycoside antibiotics in feeds and animal 

products. The sample is homogenized with a generic extraction solution and the crude soluble portion is directly injected 

into an HPLC ion-exchange column. The column effluent is then mixed with an OPA/Thiofluor reagent which forms 

highly fluorescent derivatives with the primary amine moieties of the antibiotics. 

Call if you need to analyze other Aminoglycosides. 

Extraction Procedure: 

Take one part feed:10 parts Extraction solution (w/v) 

Catalog No. 1700-1118 and homogenize for five minutes. 

Centrifuge for 10 minutes. Three layers will form: the pellet, 

a supernatant emulsion and a soft layer of floating fat. Carefully 

lift the floating fatty layer with a spatula and discard. 

Transfer the emulsion to a sealable vial. Coagulate the 

emulsion by placing the vial in a boiling water bath for 15 

minutes. Centrifuge for 10 minutes. The clear supernate is 

filtered (0.45 um Nylon) and placed in an autosampler vial. 

METHOD 


Column: ALKION cation-exchange, K+ form, 4 x 150 mm, 


ALKION Guard column, 3 x 20 mm, 


Apramycin 

Neomycin 

Gentamicin 

Complex 



Mobile Phase: 

1700-1101, Potassium buffer, K01 

1700-1102, Potassium titrant, K02 

1700-1103, Potassium ionic strength adjuster, K03 





Reagent: o-Phthalaldehyde/Thiofluor + Brij 35® 


Detection: Fluorometer 

λ ex 

: 330 nm, λ em 

: 465 nm 

“ALKION” and Thiofl uor are trademarks of Pickering Laboratories, Inc. 

“Brij 35” is a registered trademark of ICI Americas, Inc. 

0 

5 10 15 20 25 30 

min 

CONDITIONS FOR SEPARATION OF APRAMYCIN 

GENTAMICIN AND NEOMYCIN 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 60 40 0 

15 13.2 8.8 78 

15.1 12 10 78 

30 12 10 78 

30.1 0 22 78 

31 0 22 78 

31.1 60 40 0 

37 60 40 0 




REFERENCES: 

Driver, J. L., Thex, N., Raynie, D., Ofi tserova, M., Pickering, M. Single-Laboratory Validation for 

the Quantifi cation of Neomycin B and Neomycin C in Animal Feeds by Liquid Chromatography 

Fluorescence Detection with Post-column Derivatization. 

(2009) J.AOAC Int, 92, 34-41


Standard obtained 

from Sigma 

Apramycin 

APRAMYCIN ANALYSIS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 67 33 0 

5 67 33 0 

15 14.7 7.3 78 

20 14.7 7.3 78 

20.1 0 22 78 

21 0 22 78 

21.1 67 33 0 

SAMPLE: 11.1 nmole Apramycin on column 

0 

10 20 30 

min 

Standard obtained 

from Sigma 

Gentamicin Complex 

{ 

GENTAMICIN ANALYSIS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 43 31 26 

20 9 13 78 

30 9 13 78 

30.1 0 22 78 

31 0 22 78 

31.1 43 31 26 

SAMPLE: 5 x 10-6 g Gentamicin complex on column 

83 

0 

10 20 30 

min 

Standard obtained from 

US Pharmacopeia 

Neomycin 

NEOMYCIN ANALYSIS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % 

0 60 40 0 

15 13.2 8.8 78 

25 11 11 78 

25.1 0 22 78 

26 0 22 78 

26.1 60 40 0 

SAMPLE: 2.75 nmole Neomycin 

0 

10 20 30 

min 



10/11


102 / ANALYSIS OF BIOGENIC AMINES 

FOOD AND PHYSIOLOGICAL SAMPLES 

Histamine and other polyamines such as Putrescine, Cadaverine, Spermidine and Spermine are products of decomposition. 

The level of histamine, in particular, has been used as a regulatory guideline for degree of decomposition in fish. Also, 

Histamine and Tyramine levels are of interest to producers of wine and cheese. 

The polyamines, Spermidine, Spermine and Putrescine are elevated in neoplastic and damaged, e.g., burned tissue. In fact, 

the level of circulating and urinary polyamines is an indicator of total body damage. The levels can be monitored for the 

effect of diet and other modalities on recovery. 

This method employs ion-exchange chromatography so, after extraction, centrifugation/filtration are the only sample 

preparations necessary. 

Extraction Procedure 

Weigh 10 g of sample into a small glass blender cup (any 

fortifications should be added at this time). Add 50 mL of 

extraction solution, 80 % HPLC Methanol and 20 % 0.1 

N Hcl, and homogenize for two minutes. Centrifuge for 

five minutes. Mix equal portions of the supernatant with 

the mobile phase (K600). Allow to coagulate at -4 °C for 15 

minutes, then centrifuge for five minutes. The clear supernatant 

is filtered (0.45 μm Nylon) and placed in an autosampler 

vial. 

METHOD 


Column: ALKION cation-exchange, K+ form, 

4 x 150 mm, Catalog No. 9410917 

ALKION Guard column, 3 x 20 mm, 




Mobile Phase: K600, K563, K130 


Post-Column System: Pinnacle PCX 



Reagent: 300 mg of OPA, 2 g of Thiofluor, 

3 mL of 30 % Brij® 35 in 950 mL of OD104 



λ ex 

: 330 nm, λ em 

: 465 nm


Histamine 

5 Days Old 

5 Days Old 

Fresh 

Putrescine 

Cadaverine 

Histamine 

Fresh 

Putrescine 

Cadaverine 

TIME (Min) K600 % K130 % 

0 100 0 

12 100 0 

12.1 0 100 

15 0 100 

15.1 100 0 

20 100 0 

0 

2 4 6 8 10 

1 Histamine 

2 Putrescine 

3 Cadaverine 

Ahi Tuna 

min 

0 

2 4 6 8 10 

Mahi-Mahi 

min 

85 

3 4 

2 

5 

0 

1 

5 10 15 20 

min 

Gradient Separation of Polyamine Mixture 

TIME (Min) K600 % K563 % K130 % 

0 100 0 0 

6 100 0 0 

15 0 100 0 

21 0 100 0 

21.1 0 0 100 

23 0 0 100 

23.1 100 0 0 

29 100 0 0 

1 

2 

3 

Histamine 

Putrescine 

Cavaverine 

4 

5 

Spermidine 

Spermine 



10/11


120 / BROMATE ANALYSIS 

ANALYSIS OF BROMATE IN DRINKING WATER BY HPLC AND POST-COLUMN DERIVATIZATION 

Bromate is a disinfection by-product that is formed when Ozone reacts with naturally occurring Bromide in drinking 

water. Bromate is a known animal carcinogen and has also been listed as a group 2B toxin: probable human carcinogen. 

The U.S.EPA Method 300.1 employs conductivity as the means of detection which works well for most anions. However, 

the method is non-specific and coeluting interferences cannot be identified. The more recent U.S.EPA Method 317.0 

utilizes a Bromate specific reagent in a post-column reaction. This allows for a very specific and sensitive assay for 

Bromate in complex matrices. 

METHOD 

Equipment 

• LC with a binary pump 

• UV/VIS detector 

• Pickering Laboratories dual reagent PCX5200 Post-column 

derivatization instrument 

• Pickering Laboratories anion-exchange column, 4.6 x 150mm 

(Cat. No. 0785150) 

Reagents 

• 9.0 mM Sodium carbonate 

• Conc. Nitric acid (70 %) 

• Potassium bromide 

• o–Dianisidine dihydrochloride 

• Methanol 

LC Conditions 

LC Column Temperature: 42˚C 


LC Flow Rate: 1.3 mL/min 

Mobile Phase: 9 mM Na 2 

CO 3 


Post-column system: Pinnacle PCX 


Reactor Temperature: 60˚C 

Reagent: 

o–Dianisidine dihydrochloride (Add 40 mL of 70 % HNO 3 

to 300mL deionized water in a 500mL volumetric flask. Dissolve 

2.5g KBr in this solution. Dissolve 250 mg of 

o–Dianisidine dihydrochloride in 100 mL of Methanol and 

add to the Nitric acid/KBr solution and dilute to volume.) 


Detection: UV/VIS detector, λ max = 450 nm 

REFERENCES 

U.S.EPA Method 317.0. Determination of inorganic oxyhalide disinfection by-products in 

drinking water using ion chromatography with the addition of a post-column reagent for trace 

bromate analysis. 

H.P.Wagner, B.V.Pepich, D.P.Hautman and D.J.Munch, J.Chromatography A, 882 (2000) 

309 – 319. 

C.R.Warner, D.H.Daniels, F.L.Joe and G.W.Diachenko, Food Additives and Contaminants, 

vol. 13, No.6 (1996) 633 – 638.


9000 

7000 

y = 705x + 6.6128 

R 2 = 0.9998 

Area 

5000 

3000 

1000 

2 6 10 

Concentration (ppb) 

Calibration Curve 

uAU 

2.0 

10ppb 

1.5 

8ppb 

1.0 

0.5 

5ppb 

87 

0.0 

2.5ppb 

-0.4 

1.50 1.75 2.00 2.25 2.50 2.75 3.00 

min 



10/11


108 / POST-COLUMN LC ANALYSIS OF 23 N-METHYL CARBAMATES 

AN EXPANDED METHOD USING WATER/ACETONITRILE OR WATER/METHANOL GRADIENTS 

There are a number of carbamate pesticide compounds employed worldwide which are not included in the 10 compounds 

mandated by USEPA Method 531.1 and AOAC Protocol 29.A05. 

This Application Note describes methods for separating as many as 23 compounds, using a Pickering C8, 5 μm silica, column 

with either Water/Acetonitrile or Water/Methanol gradients. Instruments, post-column derivatization and detection 

protocols, and reagents remain unchanged, as described Pickering’s brochure, Post-column LC Systems for Environmental Pesticide 

Analysis (B-CA5) and User’s Manual 0101-0002. 

Although there are only two co-elutions in each of the solvent gradient protocols, the compounds were assigned to two 

separate test mixtures, A and B, in order to demonstrate the full range of separation. 

The differences in selectivity between the Acetonitrile and Methanol protocols enable the user to employ both solvent 

systems as a means of confirming peak identification. 

The following comparison of Acetonitrile to Methanol might be helpful in choosing which solvent system will be for used 

routinely, and which will be for confirmation: 

• Acetonitrile generally exhibits higher sensitivity and baseline noise. 

• It also costs more and its disposal is more restricted. 

Eleven of the most widely occurring carbamate compounds and BDMC (internal standard) are supplied by Pickering 

Laboratories in its qualitative test mixtures, Cat. No. 1700-0063. These are designated by * in the test mixture tables below. 

Other compounds may be purchased from suppliers of environmental standards. A few may vary considerably in homogeneity 

and availability. 

Water/Acetonitrile 

Water/Methanol 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

12 

13 

14 

15 

16 

17 

18 

19 

20 

21 

22 

23 

Aldicarb sulfoxide (Standak) 


Oxamyl (Vydate) 

Methomyl (Lannate) 

3-Hydroxy carbofuran 

Aldicarb (Temik) 

Propoxur (Baygon) 

Carbofuran (Furadan) 

Carbaryl (Sevin) 

Methiocarb (Mesurol) 

BDMC internal standard 

Butocarboxim sulfoxide 

Butocarboxim sulfone 

Ethiofencarb sulfoxide 

Ethiofencarb sulfone 

Butocarboxim 

Ethiofencarb 

Thiofanox Sulfoxide 

Thiofanox Sulfone 

Methiocarb sulfoxide 

Methiocarb sulfone 

3-Ketocarbofuran 

Thiofanox


Analytical Conditions: Water/Acetonitrile Protocol 

Column: 4.0 x 250 mm, Pickering Cat.No. 0840250 

Sample Injection: 10 μl in MeOH (for all chromatograms shown) 

Mobile Phase: 

Solvent A: HPLC-grade Water 

Solvent B: HPLC-grade Acetonitrile (ACN) 

Program: 

TIME H 2 

O % ACN % GRADIENT 

0 90 10 

2 90 10 Isocratic 

46 49 51 Linear 

46.1 30 70 Step 


Equilibration Time: 13 minutes 

Eluant Flow Rate: 0.80 mL/minute 

Column Temperature: 37 ˚C 

Reagent 1: 0.3 mL/minute, CB130 Hydrolysis Reagent 

Reagent 2: 0.3 mL/minute, OPA reagent 1 for derivatization 

Reactors: 

Hydrolysis: 100 ˚C, 500 μL 

Derivatization: Ambient, 100 μL 

Flourescence Detection: 

Excitation: 330 nm 

Emission: 465 nm 

Analytical Conditions: Water/Methanol Protocol 

Mobile Phase: 

Solvent A: HPLC-grade Water 

Solvent B: HPLC-grade Methanol (MeOH) 

Program: 

TIME H 2 

O % MeOH % GRADIENT 

0 88 12 


42 34 66 Linear 


46.1 0 100 Step 


89 

1 o-Phthalaldehyde (O120) and Thiofl our (3700-2000), Pickering’s 

brand of Dimethylaminoethanethiol•HCI, in OPA Diluent (CB910) 

REFERENCE: 

R.T. Krause, J. Chromatography, 185, 615-624 (1979) 

R.T. J. Chromatographic Science, 16, 281-288 (1978) 

H.A. Moye, S.J. Scherer and P.A. St. John, Anal. Letters, 10, 1049-1073 (1977) 

A. de Kok and M. Hiemstra, J. AOAC int’l, 75, 1063-1072 (1992) 

Offi cial Methods of Analysis, 15th Ed., AOAC Int’l, Arlington, VA, (291-293) 

Method 531.1, U.S. Environmental Protection Agency 



10/11


112 / EXPANDED HPLC METHOD FOR N-METHYL CARBAMATES 

POST-COLUMN ANALYSIS OF PESTICIDES IN FOOD AND POTABLE WATER SAMPLES 

Carbamate pesticides are widely used around the world to protect crops. In addition, they are used as biocides for industrial 

or other applications and in household products. Though carbamates are biodegradable compounds and bioaccumulation 

usually happens only to a low extend it is important to monitor produce to make sure enough time has elapsed between 

harvest and applying pesticides. Also, because of their high solubility, carbamates can leach into ground waters in porous 

soils and consequently find their way into drinking water supplies. 

As part of FDA’s pesticide monitoring program individual lots of domestic and imported foods and feeds are sampled and 

tested for pesticide residues to enforce the tolerances set by EPA. There are 11 compounds mandated by USEPA Method 

531.2 for drinking water but they represent only a fraction of the carbamates that require monitoring in domestic and 

imported products. Methyl carbamates are separated using a reversed-phase column and then readily react with o-Phthalaldehyde 

and a mercaptan after hydrolysis to form highly fluorescence compounds. This post-column reaction is the basis for 

official EPA Method 531.2 and AOAC Method 985.23. 

This new expanded method is suitable for detecting a wide range of carbamates; post-column derivatization with fluorescence 

detection is a sensitive and selective method for residue analysis in water, food and feed samples. This method 

employs the same HPLC and post-column equipment and chemicals as USEPA Method 531.2 and will allow laboratories 

to increase the range of tested compounds. 

The separation is achieved on a C 8 

stationary phase with a water/Methanol gradient. Differences in selectivity of a water/ 

Acetonitrile gradient may be used for confirmation. 

METHOD 


Column: Expanded resolution C 8 

analytical column 4x250 mm, 

P/N 0840250 Guard column P/N 18ECG001 


Column Temperature: 50 ˚C 

Mobile Phase: Water/Methanol 


Post-column System: Pinnacle PCX or Vector PCX 


Reactor Temperature: 100 ˚C 

Reagent 1: CB130 

Reagent 2: 100 mg of OPA and 2 g of Thiofluor in 950 mL 

of CB910 Diluent 


Detection: 

Fluorescence Detector 

λ ex 

= 330 nm, λ em 

= 465 nm 

HPLC Gradient: 

TIME WATER % METHANOL % 

0 85 15 

2 85 15 

42 30 70 

46 30 70 

46.1 0 100 

50 0 100 

Equilibration: 10 min 

ACKNOWLEDGEMENTS: 

Pickering Laboratories would like to thank John Casanova and Frank Schenck (FDA) for 

their help with this project.


1 3 

5 

6 

7 

8 9 

15 

19 

20 

21 

22 

27 

26 28 

29 

30 

31 

32 

0 10 20 30 40 

min 

2 

4 

10 

11 

12 13 14 

16 

17 

18 

23 

24 

25 

33 34 

35 

0 10 20 30 40 min 

91 

5 

15 

22 

35 

1 2 3 

7 

6 

8 9 

20 

21 

26 

33 32 

30 

31 

0 10 20 30 40 min 

List of Carbamates 

1 

2 

3 

4 

5 

6 

7 

8 

9 


Butoxycarboxim 


Oxamyl 

Methomyl 

Ethiofencarb sulfone 

Ethidimuron 

Thiofanox sulfoxide 

Thiofanox sulfone 

10 

11 

12 

13 

14 

15 

16 

17 

18 

Formetanate HCl 

Ethiofencarb sulfoxide 

Dioxacarb 


Butocarboxim 

Aldicarb 

Metolcarb 

Cloethacarb 

Bendiocarb 

19 

20 

21 

22 

23 

24 

25 

26 

27 

Carbetamide 

Propoxur 

Carbofuran 

Carbaryl 

Ethiofencarb 

Thiofanox 

Banol 

Isoprocarb (MIPC) 

2,3,5-Trimethacarb 

28 

29 

30 

31 

32 

33 

34 

35 

3,4,5-Trimethacarb 

Fenobucarb (BPMC) 

Methiocarb 

BDMC 

Bufencarb 

Promecarb 

Zectran 

Aminocarb 



10/11


325 / ANALYSIS OF FORMALDEHYDE 

SIMPLE AND SENSITIVE METHOD FOR ANALYZING FREE FORMALDEHYDE 

Formaldehyde is used for disinfections of industrially manufactured products. However, it is detrimental to health and its 

potential oncogenicity led to the current utilization of a formaldehyde donor for long-term preservation. Such donors are in 

hydrolytic equilibrium with formaldehyde producing free formaldehyde on exposure to moisture. 

This method is used to quantify the free formaldehyde without upsetting the existing equilibrium. Potential interferences 

are either separated from formaldehyde or they don’t react with the post-column reagent. The lutidine derivative is highly 

fluorescent resulting in ppm detection levels. 

METHOD 


Column: Diol, 5 μm, 4.6 x 250 mm 



Mobil Phase: Acetonitrile: Water (85:15) isocratic 





Reagent: 

0.81 M Ammonimn acetate 

0.12 M Galcial acetic acid (pH=5) 

0.05 M 2, 4-Pentanedione with water 



λ ex 

: 412 nm, λ em 

: 510 nm 

4.0 6.0 8.0 10.0 12.0 14.0 16.0


206 / GLYPHOSATE AND AMPA ANALYSIS IN CROPS 

A SIMPLE AND REPRODUCIBLE EXTRACTION AND CLEAN-UP 2 FOR HPLC POST-COLUMN DERIVATIZATION 

The recently practiced method1 for analysis of Glyphosate and AMPA in crops suffers from an expensive, time consuming 

clean-up procedure that has less than ideal recoveries. Although the analysis (after clean-up) by ion-exchange chromatography 

with post-column derivatization is rugged and sensitive, a new method was sought to improve the sample preparation. 

This resulted in AOAC Method 2000.52 2 which has a streamlined clean-up followed by pre-column derivatization and 

GC/MS analysis. We show how this simplified sample preparation is suitable for the classic ion-exchange/post-column 

analytical protocol. 

METHOD 

Equipment: 


• Fluorescence detector 

• Pickering Laboratories Vector PCX or Pinnacle PCX Post-column 

Derivatization instrument 

• Pickering Laboratories Potassium cation exchange column, 

4.0 x 150 mm (Cat. No. 1954150) 

• Pickering Laboratories Potassium cation exchange guard column, 

3.0 x 20 mm (Cat. No 1953020) 

• Cation exchange clean-up column (Cat. No 1705-0001) 

Reagents: 

• Potassium eluant (Cat. No.K200) 

• Potassium regenerant (Cat. No. RG019) 

• Hypochlorite diluent (Cat. No. GA116) 

• o-Phthalaldehyde diluent (Cat. No. GA104) 

• Thiofluor (Cat. No. 3700-2000) 

• o-Phthalaldehyde (Cat. No. O120) 

• 5 % Sodium hypochlorite solution 

• Methylene chloride 

• Acidic modifier solution 

• Clean-up column eluant 


Extraction: 

To 25 g of a homogenous sample add enough water (after 

estimation of moisture content) to make the total volume of 

water 125 mL. Blend at high speed for 3–5 min. and centrifuge 

for 10 min. Transfer 20 mL of the aqueous extract into 

a centrifuge tube and add 15 mL of methylene chloride (to 

remove nonpolar co-extractives). Shake for 2–3 min. and 

centrifuge for 10 min. Transfer 4.5 mL of the aqueous layer 

into a vial and add 0.50 mL acidic modifier solution (16g 

KH 2 

PO 4 

, 160 mL H 2 

O, 40 mL Methanol, 13.4 mL HCl). 

Shake and centrifuge for 10 min. 

Matrix Specifi c Modifi cation: 

Plants with high: 1) Water 2) Protein 3) Fat Content 

1 For crops that absorb large amounts of water, reduce test 

portion to 12.5 g keeping water volume the same. 

2 For crops that have high protein content add 100 µL HCl to 

20 mL aliquot of crude extract. Cap, shake and centrifuge 

for 10 min. 

3 For crops that have high oil content, do the methylene 

chloride partition twice. 

Cation-Exchange Clean-up: 

Transfer 1 mL of extract (representing 0.18 g normal crop 

or 0.09 g dry crop) to the column reservoir and elute to the 

top of the resin bed. Add 0.70 mL of the elution solution 

(160 mL H 2 

0, 2.7 mL HCl, 40 mL Methanol) and discard 

the effluent. Repeat with a second 0.70 mL portion and 

discard effluent. Elute with 12 mL of the elution solution 

and collect in a round-bottomed flask. Evaporate to dryness 

in a water bath set at 40 ˚C using a rotary evaporator. Or 

collect in a centrifuge tube and evaporate using a vacuum 

vortex evaporator. Dissolve residue in 2.0 mL of the elution 

solution (use 1.5 mL for dry crops). Extracts before evaporation 

can be stored refrigerated for up to 7 days. 

93 

Continued on next page 



10/11


206 continued from previous page 

LC Conditions 

LC Column Temperature: 55 ˚C 


LC Flow Rate: 0.40 mL/min 

Mobile Phases: 

K200, Potassium Eluant 

RG019, Regenerant 


Post-column System: Vector PCX or Pinnacle PCX 


Reactor Temperature: 36 ˚C 

Reagent 1: 100μL of 5 % NaOCl (Bleach) in GA116 Diluent 

Reagent 2: 100 mg o-Phthalaldehyde and 2g Thiofluor in 

950 mL GA104 Diluent 

Flow rate: 0.3 mL/min 

Detection: Fluorescence detector 

λ ex 

: 330 nm, λ em 

: 465 nm 

ACKNOWLEDGEMENT: 

We thank The Montana Department of Agriculture, Laboratory Bureau for extracting 

alfalfa, tomato and other samples. 

REFERENCES: 

1) “Validation of an Analytical Residue Method for Analysis of Glyphosate and Metabolite: 

An Interlaboratory Study.” J. Agric. Food Chem. 34, (1986) 955–960. 

2) P.L. Alferness and L.A. Wiebe, “Determination of Glyphosate and Aminomethylphosphonic 

Acid in Crops by Capillary Gas Chromatography with Mass-Selective Detection: 

Collaborative Study.” Journal of AOAC International, 2001 84, 823–846. 

STEP TIME (Min) INTERVAL (Min) K200 % RG019 % 

0 0 0 100 0 Injection 

1 0-15 15 100 0 Isocratic 

2 15.1-17 1.9 0 100 Step Change 

3 17.1-25 7.9 100 0 Re-Equilibration 

NOTE: Ensure that the auto sampler injection valve is fi tted with a Tefzel or PEEK rotor 

seal for compatibility with the high-pH column regenerant. 

Heavy-metal ions, especially iron can cause loss of sensitivity. Contamination of the guard 

and column can be removed by using RESTORE (Cat. No. 1700-0140) reagent. 

Glyphosate 

AMPA 

Alfalfa spiked with 

0.2 ppm glyphosate 

Tomato spiked with 

0.15 ppm glyphosate 

2 4 6 8 10 12 14 16 18 20 22 24 

Time [min] 

Chromatograms of Alfalfa and Tomato matrix spiked with glyphosate and AMPA


123 / SIMULTANEOUS DETERMINATION OF NITRITE AND NITRATE IN PROCESSED FOODS 

SIMULTANEOUS DETERMINATION OF NITRITE AND NITRATE IN BABY FOODS 

Saji George, Maria Ofitserova, Ph. D., Michael Pickering, Ph. D. 

Nitrite either alone or in combination with nitrate is added to food to preserve the color and taste and to prevent foods from 

becoming rancid. They are also used in food for their anti-microbial properties. Higher levels in vegetables and leafy greens 

are possible from the use of nitrate fertilizers and/or livestock manure. 

Nitrate can be reduced to nitrite at certain physiological conditions in the human body. Nitrite however can oxidize Fe (II) in 

hemoglobin to methemoglobin, an Fe (III) product. The oxidized product is incapable of binding molecular oxygen and 

high concentrations of methemoglobin can result in methemoglobinemia especially in infants. Nitrite can also react with 

secondary amines present in food products or in the digestive system to form nitrosamines, a class of carcinogenic compounds. 

Nitrite levels in food could also be produced by reduction of nitrate to nitrite during processing. 

The AOAC Official Method 993.03 1 for the analysis of nitrate involves reduction using spongy Cadmium which is toxic and 

carcinogenic. The USFDA improved on this method by using Vanadium (III) chloride and heat 2 for the post-column reduction 

of nitrate to nitrite. Nitrite reacts with this modified Griess reagent to produce a red chromophore with maximal absorbance 

at 535 nm. Pickering Laboratories Inc. has further improved this method by substituting the corrosive and volatile 

hydrochloric acid with methane sulfonic acid. 

METHOD 

Equipment 


• UV/VIS detector 

• Pickering Laboratories single reagent Pinnacle PCX 

post-column derivatization unit 

(1153-1021 - 120 V, 1153-1022 – 240 V) 

• Pickering Laboratories anion exchange column, 

4.6 X 150 mm (Cat. No. 0785150) 

Chemicals 

• Sodium acetate 

• Vanadium(III)chloride 

• N-(1-Naphthyl)ethylenediamine dihydrochloride 

• m-Nitro aniline 

• 20 %(v/v) methane sulfonic acid 

LC Conditions 


LC Flow Rate: 1 mL/min 

Mobile Phase: 0.15 M sodium acetate 


Column Temperature: 50 °C 




Reagent: 

Mix 50 mL of (i) and (ii), and 1.25 mL of (iii) and dilute to 

250 mL using 20 % methanesulfonic acid 

[ (i) 1 % Vanadium(III)chloride in 20 % methanesulfonic 

acid, 

(ii) 1 % m-Nitro aniline in 20 % methanesulfonic acid, 

(iii) 1 % N-(1-Naphthyl)ethylenediamine dihydrochloride in 

20 % methanesulfonic acid ] 

Detector: UV/VIS, λ max 

= 535 nm 

Extraction Procedure 

Fruits and Vegetables 

To 5 g of baby food in a 50 mL centrifuge tube add 25 

mL of 50-60 ºC water (for vegetables) or 15 mM Sodium 

acetate (for fruits) and shake for 10 min. Add 12.5 mL of 

acetonitrile and make up the volume to 50 mL using water 

(for vegetables) and sodium acetate (for fruits). Centrifuge 

the mixture for 15 mins at 5000 rpm. Filter the supernatant 

through a 0.45 μ nylon filter and dilute to fall within the 

linear range. 

Processed Meat 

To 5 g of homogenized processed meat in a blender add 25 

mL of 50-60 ºC water and blend for 2 min. Add 25 mL of 

acetonitrile and blend for an additional 2 min. Transfer into 

a beaker and make up the volume to 100 mL using warm 

water. Filter the 

mixture using Whatman filter paper. Filter further through a 

0.45 μ nylon filter and dilute to fall within the linear range. 

NOTES 

Post-column reagent solutions are stored in plastic or Tefl on containers. 

All solutions are fi ltered through 0.45 µ nylon fi lter before use. 

Nitrate/Nitrite standards should be checked prior to use for oxidation. 

Sample pH should be checked to determine the choice of extraction solution 

since acidic pH facilitates the conversion of nitrite to nitrate. 

95 




10/11



10 ppm nitrite-nitrate standard using (a) 20 % 

methanesulfonic acid , (b) 20 % hydrochloric acid 

Nitrite 

Nitrate 

a) 

b) 

0 10 20 

min 

(a) 0.05 ppm, (b) 0.01 ppm nitrite-nitrate standard 

Nitrite 

a) 

b) 

Nitrate 

0.0 5.0 10.0 15.0 min 

Calibration Curve for Nitrite-Nitrate (0.05 ppm – 50 ppm) 

25000000 

20000000 

y (nitrite) = 377491x + 204536 

R 2 = 0.9986 

15000000 

10000000 

y (nitrate) = 166790x - 17901 

R 2 = 0.9985 

5000000 

0 

0 

Nitrite 

Nitrate 

10 20 30 40 50 60


Banana (baby food): (a) spiked with 100 ppm 

nitrite-nitrate solution, (b) blank 

Carrot (baby food): (a) spiked with 100 ppm 

nitrite-nitrate solution, (b) blank 

Nitrate 

b) 

a) 

Nitrate 

a) 

Nitrite 

b) 

0.0 5.0 10.0 15.0 min 

Nitrite 

Corned Beef: (a) spiked with 50 ppm nitrite and 

500 ppm nitrate solution, (b) blank 

0.0 5.0 10.0 15.0 min 

Nitrate 

a) 

b) 

Nitrite 

0.0 5.0 10.0 15.0 min 

97 

SAMPLE 

SPIKED 

CONC. 

(PPM) 

NITRITE 

CAL. 

CONC. 

(PPM) 

RECOVERY DATA 

RECOVERY 

(%) 

SPIKED 

CONC. 

(PPM) 

NITRATE 

CAL. 

CONC. 

(PPM) 

RECOVERY 

(%) 

Baby Food 

Carrot 100 92.25 92 100 79.3 79 

Banana 100 94.6 95 100 102.5 103 

Processed 

Food 

Corned 

Beef 

50 54.4 109 500 478.9 96 

ACKNOWLEDGMENTS 

John A. Casanova, Food and Drug Administration, 60 8 th Street, Atlanta, GA 30309 

REFERENCES 

1. 1. AOAC- Offi cial Methods of Analysis of AOAC International (2000) 17th Ed., Section 50.1.11. 

2. Use of Griess Reagents Containing Vanadium (III) for the Post-column 

Derivatization and Simultaneous Determination of Nitrite and Nitrite in Baby Food, 

John A. Casanova, Lois K. Gross, Sarah E. McMullen and Frank Schenck, 

Food and Drug Administration, 60 8th Street, Atlanta, GA 30309. 



10/11


105.2 / PARALYTIC SHELLFISH TOXINS 

ANALYSIS OF DINOFLAGELATE DERIVED NEUROTOXINS IN BIVALVE MULLUSKS USING 

HPLC POST-COLUMN FLUORESCENCE METHOD 

The paralytic shellfish toxins are a group of 18 secondary metabolites deposited in bivalve mollusks by dinoflagelates. Dinoflagelates 

blooms are seasonal, occurring during warm months. Since it is unpredictable whether an infestation will occur, 

the shellfish population should be regularly monitored for toxins. Ingestion of contaminated shellfish can lead to paralytic 

shellfish poisoning; a life-threatening illness. 

Mouse bioassay is the official method of AOAC International, but the drawbacks associated with this method have led to 

exploration of chemical methods. The most common HPLC post-column method is to oxidize the separated toxins under 

alkaline conditions to a fluorescent compound. Sullivan et al. 1 used this method to determine the gonyautoxins 1-6 (GTX1- 

6), saxitoxin (STX) and neosaxitoxin (neoSTX) but not the N-sulfocarbamoyl-11-hydroxysulfate toxins (C1-C4). Oshima et 

al. 2, 3 modified this method to determine the 3 toxin groups separately using isocratic elution with 3 different mobile phases. 

Further improvement by Jeffery van de Riet et al. 4, 5 has led to a shorter analysis time to determine the 3 groups of toxins 

using step gradient and a switching valve. This method abstract describes the use of Pickering Laboratories Pinnacle PCX 

post-column derivatization system for the HPLC post-column determination of paralytic shell fish toxins. 

METHOD 

Equipment: 

• LC with a Binary Pump 

• Fluorescence Detector 

• Pickering Laboratories dual reagent 

Pinnacle PCX post-column derivatization unit 

(1153-1061 – 120 V, 1153-1062 – 240 V) 

• Agilent Technologies Zorbax Bonus RP column, 

3.5 µm, 4.6 x 150 mm (863668-901) 

Reagents: 

• Heptane Sulfonate 

• Phosphoric Acid 

• Ammonium Hydroxide 

• Acetonitrile 

• Periodic Acid 

• Sodium Hydroxide 

• Nitric Acid 

LC Conditions 



Mobile Phase: 

A. 11 mM heptane sulfonate, 5.5 mM phosphoric acid, adjusted 

to pH 7.1 with ammonium hydroxide. 

B. 11 mM heptane sulfonate, 16.5 mM phosphoric acid, 11.5 % 

acetonitrile, adjusted to pH 7.1 with ammonium hydroxide. 


Column Temperature: 40 °C 

Reactor Volume: 1.0 mL (knitted) 



Reagents: 

1. 100 mM phosphoric acid, 5 mM periodic acid, 

adjusted to pH 7.8 with 5 M sodium hydroxide 

2. 0.75 M nitric acid 


λ ex 

: 330 nm, λ em 

: 390 nm


GTX4 

GTX1 

dcGTX3 

GTX5 dcGTX2 

neoSTX 

dcSTX 

STX 

GTX2 

GTX3 

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 

Chromatogram of GTX and STX mixed working standard 

HPLC GRADIENT PROGRAM 

TIME (Min) %A %B 

0 100 0 

7.9 100 0 

8 0 100 

18.5 0 100 

18.6 100 0 

24 100 0 

99 

REFERENCES: 

1) J.J.Sullivan et. al 

J.Food Sci. 50(1) (1985) 26-29. 

2) Yasukatsu Oshima 

“Post-column Derivatization Liquid Chromatographic Method for Paralytic Shellfi sh Toxins.” 

Journal of AOAC International Vol.78, No.2, (1995) 528-532. 

3) Yasukatsu Oshima, et. al 

In Mycotoxins and Phytotoxins “88, Elsevier Science Publishers, 

Amsterdam, The Netherlands, 319-326. 

4) Wade A. Rourke, et. al 

“Rapid Post-column Methodology for Determination of Paralytic Shellfi sh Toxins in Shellfi sh Tissue.” 

Journal of AOAC International Vol.91, No.3, (2008) 589-597. 

5) Jeffrey M. van de Riet, et. al 

“Liquid Chromatographic Post-column Oxidation Method for Analysis of Paralytic Shellfi sh Toxins in 

Mussels, Clams, Scallops and Oysters: Single-Laboratory Validation:” 

Journal of AOAC International Vol.92, No. 6, (2009) 1690-1704. 



10/11


104 / POLYETHER ANTIBIOTICS 

MONENSIN, NARASIN, & SALINOMYCIN 

Polyether antibiotics, such as Monensin, Narasin, and Salinomycin, in raw material, premix, liquid supplements, and 

final feeds, are best quantified using a high-performance liquid chromatograph equipped with a post-column reactor. The 

polyether antibiotics react, post-column, with Vanillin in the presence of Sulfuric acid, and the resulting products can be 

detected at 520 nm. However, this corrosive reagent can adversely degrade most instruments. Pickering’s special Post-column 

Systems, comprised of non-metallic parts and washing pump head, offers resistance to sulfuric acid. Special operating 

instructions will also be included with this instrument. Contact Pickering Laboratories for more information about the Inert 

Vector PCX Post-column System for polyether antibiotics. 

METHOD 


Column: Polyether reversed-phase column, C 18 

, 

4.6 x 250 mm, Catalog No. 2381750 



Mobile Phase: Methanol/5 % acetic acid in 

water (90/10), Isocratic 


Post-column System: Vector PCX or Pinnacle PCX 

(Non-metallic post-column derivatization system is required) 

Reagent 1: Concentrated Sulfuric acid/Methanol (4:96 v/v) 

Reactor 1: Ambient, 0.1 mL 

Reagent 2: 60 g of Vanillin (or p-dimethylaminobenzaldehyde) 

in 950 mL of Methanol 



Detection: UV-Vis detector: 

Vanillin λ=520 nm, DMAB λ=450 nm 

Monensin B 

0 5 10 15 20 25 

Monensin A 

Salinomycin 

Narasin 

min 

REFERENCES 

1) M.R. LaPointe and H. Cohen, J.A.O.A.C., 71 (1988) 480–484. 

2) J.M. Rodewald, J.N. Moran, A.L. Donoho, and M.R. Coleman, 

J.A.O.A.C., 75 (1992) 272–279.


117 / PARAQUAT AND DIQUAT IN WATER 

DIRECT INJECTION METHOD 

Paraquat and Diquat are widely used in agriculture and industry. They are employed as contact herbicides, as harvest aids, 

for pasture renovation and for aquatic weed control. Their environmental persistence allows for contamination of water 

supplies; wells, lakes, rivers, etc. Since Paraquat is a Class I Toxin and Diquat is a Class II Toxin, water resources must be 

monitored for the presence of these herbicides. 

This method employs ion exchange chromatography so filtration is the only sample preparation necessary. Also, since both 

Paraquat and Diquat have a fixed +2 charge, 2 mL can be injected without peak degradation. Post-column derivitization 

improves sensitivity and greatly improves selectivity by shifting the detection to longer wavelengths. 

METHOD 


Column: ALKION column 4 x 150mm, 

Catalog No. 9410917, Guard column 3 x 20mm, 




Mobile Phase: 

1700-1101, Potassium buffer, K01 

1700-1102, Potassium titrant, K02 

1700-1103, Potassium ionic strength adjuster, K03 

Injection Volume: 2 mL 



Reaction Dwell: 0.15 mL 


Reagent: 300 mg/L Sodium Dithionite in 

0.3 M Sodium Hydroxide 


Detection: 395 nm for Paraquat and 378 nm for Diquat 

Without post-column derivatization: 

257 nm for Paraquat and 308 nm for Diquat 

Diquat 

Paraquat 

0 2 4 6 8 10 12 

CONDITIONS 

TIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % IPA % 

0 31.5 53.5 0 15 

10 28 47 10 15 

15 28 47 10 15 

15.1 0 85 10 5 

17 0 85 10 5 

17.1 31.5 53.5 0 15 

min 

101 



10/11


124 / SULFONAMIDE ARTIFICIAL SWEETENERS IN FOOD PRODUCTS 

ANALYSIS OF ACESULFAME, SACCHARIN AND CYCLAMATE BY HPLC WITH POST-COLUMN DERIVATIZATION 

Nonnutritive sweeteners are widely used in foods and beverages. Since some studies raised questions about long-term safety 

of these compounds, their concentration in food products is regulated. Among the artificial sweeteners used worldwide 

Saccharin and Cyclamate cause most controversy and their use is restricted in many countries, including the US. Liquid 

chromatography is a method of choice for artificial sweeteners but Cyclamate analysis is complicated by the fact that this 

compound does not exhibit noticeable UV absorbance. 

This method allows for simultaneous analysis of Acesulfame, Saccharin and Cyclamate by LC with post-column derivatization 

followed by fluorescence detection. Since the sweeteners are commonly used in combination, a single method of 

analysis is preferred. 

METHOD 


Analytical Column: Reversed-phase column, C18, 2x100 mm 



Mobile Phase: 

0.02 M Potassium Phosphate Monobasic in water. Isocratic 

for 8 min, followed by washing the column with 70 % ACN 

– 30 % 0.02 M KH 2 

PO 4 



Reagent: Hexadecyltrimethyl ammonium bromide (10-3 M), 

1,6 Diphenyl-1,3,5-hexatriene (4x10-6 M) 

Reactor: 40 ˚C, 0.1 mL 


Detection: Fluorescence, 

λ ex 

= 365 nm, λ em 

= 460 nm 

Acesulfame K 

Saccharin 

Cyclamate 

0 2.5 5 

7.5 min 

Chromatograms of a) Diet cola containing Acesulfame K; b) Soft drink spiked with 60 ppm of 

artifi cial sweeteners. 

b 

a 

REFERENCES 

James F. Lawrence, Analyst, vol. 112, No 6 (1987), 879-881 

James F. Lawrence, Claudette F. Charbonneau, J. Assoc. Anal. 

Chem., vol. 71, No 5 (1988), 934-937


327 / ANALYSIS OF VOGLIBOSE IN PHARMACEUTICAL FORMULATIONS 

BY HPLC WITH POST-COLUMN DERIVATIZATION 

Voglibose is an Alpha-Glucosidase inhibitor widely used for the treatment of diabetes. Alpha-glucosidase inhibitors are 

agents that delay the glucose absorption at the intestinal level and thereby prevent sudden surge of glucose after a meal. 

Vogilbose is the safest and most effective drug of its class. 

Since Vogibose has no UV chromophore, post-column derivatization is employed to produce a fluorescent derivative. 

This abstract describes a very sensitive and robust analytical method for the analysis of Voglibose in pharmaceutical tablets. 

Simple sample preparation and fast analysis time allow for using this method in high throughput environments. 

METHOD 


Column: Amino column, 

4.6x250 mm, 




Mobile Phase: Sodium phosphate buffer, 20 mM 

pH 6.5 / Acetonitrile (37 : 63) 

Injection Volume: 50 μL 


Crush 5 tablets and mix with 25 mL of mobile phase. 

Sonicate for 10 min and filter liquid portion through 

0.45 μm filter. Put in HPLC autosampler vial and inject 50 μL. 





Cooling Coil: 0.15 mL (at room temperature) 

Reagent: Taurine (6.25 g), Sodium Periodate (2.56 g) 

in 1000 mL of water 



λ ex 

: 350 nm, λ em 

: 430 nm 

103 

Repeatability studies for 

different concentration levels. 

0.5 ppm 100 ppm 

Average RT, min 21.25 21.26 

RSD, %, N = 6 0.36 0.08 

Average Peak Area 9.22 1,562.69 

RSD, %, N = 6 1.48 0.79 




10/11



3500 

3000 

y = 16.468x + 4.7493 

R 2 = 0.9998 

2500 

Peak Area 

2000 

1500 

1000 

500 

0 

0 50 100 150 200 250 

Concentration, ppm 

Calibration Curve for Analytical Range 0.5-200 ppm 

Voglibose 

0 5 10 15 20 25 


50 ppm, 50 μL Injection 

min 

Voglibose 

Voglibose 

0 5 10 15 20 25 


0.5 ppm, 50 uL Injection 

min 

0 5 10 15 20 25 

Chromatogram of Voglibose Tablets (Volix TM , 0.2 mg), 

50 μL Injection 

min

NOTES 

105 



10/11

GENERAL TERMS & CONDITIONS 

1 CONTROLLING DOCUMENT 

These T & C’s and only these T & C’s apply to all goods sold and services provided by 

Seller to Buyer. By the placement of Buyer’s purchase order, Buyer consents to these 

T & C’s and no others. 

2 TERMS OF PAYMENT 

All payments are due and payable thirty (30) days from the date of the invoice. A service 

charge of 1 1/2 % per month shall apply to all invoices not paid within 30 days. 

3 SECURITY AGREEMENT 

(a) As security for Buyer’s payment, Buyer grants Seller a purchase money security 

interest in the specific goods for which payment is due. Seller has the right to file a 

financing statement evidencing this security interest. 

(b) This security interest shall terminate upon receipt by Seller of payment for the 

specific goods. 

4 DELIVERY, TITLE AND RISK OF LOSS 

Title to goods and risk of loss of goods shall pass to Buyer when Seller delivers such 

goods to a common carrier or Buyer’s agent. Delivery shall be EX Works Seller’s Factory. 

Delivery dates agreed to by Seller are approximate only. Seller shall not be liable 

for, nor shall Seller be in breach of its obligations to the Buyer because of any delivery 

made within a reasonable time after the stated delivery date. Seller may, by written 

notice to Buyer, change any delivery date, and such date shall become the agreed 

upon delivery date unless Buyer objects to such date in writing delivered to Seller 

within ten (10) days of receipt of Seller’s notice. 

5 FORCE MAJEURE 

Seller shall not be liable for any failure to deliver, or delay in the delivery of, any 

goods or services due to any cause beyond its control, including but not limited to 

natural phenomena, government actions, fires, labor disputes, or inability to obtain 

components, energy, materials, manufacturing facilities, or transportation. In the 

event of such delay, the date of delivery or performance hereunder shall be extended 

by a period equal to the time lost by reason of such delay. In the event Seller’s production 

is curtailed for any of the above reasons, Seller may allocate its production to its 

various Buyers. 

6 SELLER’S LIABILITY 

If Buyer notifies Seller within 30 days under the terms hereof of a claimed defect, 

Buyer shall concurrently offer Seller an opportunity to investigate the claim and to 

inspect allegedly defective goods. If Seller determines that Buyer’s claim is valid, 

Seller may repair the defective goods or replace the defective goods with conforming 

goods at the Seller’s Factory. Failure to offer Seller such opportunity shall constitute 

acceptance by Buyer and waiver of all claims for defects. Seller’s liability for damages 

on account of a claimed defect in any product delivered by Seller shall in no event 

exceed the purchase price of the product delivered by Seller on which the claim is 

based. Replacement of defective goods or repayment of the purchase price for any 

such product will be made only upon return of the defective product. Specifically 

and without limiting the generality of the foregoing, Seller shall not be responsible or 

liable to buyer or to any third party for any lost profits, or incidental, consequential, 

indirect, special or contingent damages for any breach of warranty or other breach of 

Seller’s obligations under this contract. Seller shall not be liable for damages relating 

to any instrument, equipment, or apparatus with which the product sold under this 

agreement is used. 

7 SELLER’S REMEDIES 

If Buyer fails, with or without cause, to furnish Seller with specifications and/or 

instructions for, or refuses to accept deliveries of, any of the products sold under this 

contract, or is otherwise in default under or repudiates this contract or any other contract 

with Seller or fails to pay when due any invoice under this contract, then in addition 

to any and all remedies allowed by law, Seller without notice (1) may deter shipment 

under this or any contract between Buyer and Seller until such default, breach 

or repudiation is removed and/or (2) may cancel any undelivered portion of this and/ 

or any other contract in whole or in part, Buyer remaining liable for damages. 

8 PATENT INDEMNITY 

(a) Seller shall, at its own expense, defend any suit that is instituted against Buyer 

to the extent such suit alleges that any goods, other than prototypes, or any part 

thereof sold or leased hereunder infringes on any United States patent, trademark, 

or copyright (except goods covered by Section 8(b) below), provided that such 

alleged infringement does not arise from any modification or addition to the goods 

by anyone other than Seller, or the use of such goods as a part of or in combination 

with any other device or parts or from the use of such goods to practice any 

method or process. Provided further that the Buyer gives Seller immediate notice 

in writing of any such suit and permits Seller, through counsel of its choice, to 

answer the charge of infringement and defend such suit; and the Buyer gives 

Seller all the needed information, assistance, and authority, at Seller’s expense, to 

enable Seller to defend or settle such suit. In the case of a final award of damages 

in any such suit, Seller shall pay such award but shall not be responsible for any 

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sale of the goods is enjoined, Seller may at its own option and expense: (1) procure 

for Buyer the right to use, lease, or sell such goods, (2) replace such goods, (3) 

modify such goods, or (4) remove such goods and refund the purchase price paid by 

Buyer less a reasonable sum for use, damage, and obsolescence. THIS SECTION 

STATES SELLER’S TOTAL RESPONSIBILITY AND LIABILITY, AND THE 

BUYER’S SOLE REMEDY FOR ANY ACTUAL OR ALLEGED INFRINGE- 

MENT OF ANY PATENT, TRADEMARK, OR COPYRIGHT BY ANY 

GOODS DELIVERED HEREUNDER OR ANY PART THEREOF. THIS 

SECTION 8 IS IN LIEU OF AND REPLACES ANY OTHER EXPRESS, 

IMPLIED OR STATUTORY WARRANTY AGAINST INFRINGEMENT. 

(b) The Buyer shall, at its own expense, indemnify and hold Seller harmless from 

and against any expense or loss resulting from any infringement of any patent, 

trademark, or copyright arising as a result of Seller’s compliance with any of 

the Buyer’s designs, specifications, or instructions, and shall defend at its own 

expense including attorney fees, any suit brought against Seller alleging any such 

infringements provided that Seller (i) gives the Buyer immediate notice of any such 

suit and permits the Buyer through counsel of its choice, to defend such suit, and 

(ii) gives Buyer all needed information, assistance, and authority, at the Buyer’s 

expense, necessary for the Buyer to defend any such suit. 

9 ORDER CANCELLATION 

Buyer may, at any time, cancel its purchase of goods or services but only by paying 

15 % of the purchase price if such cancellation is received by Seller more than 90 

days before the scheduled delivery date, 50 % if received more than 45 days but less 

than 90 days, 75 % if received more than 15 days but less than 45 days, and 100 % if 

received less than 15 days. 

10 WARRANTY AND LIMITATION OF LIABILITY 

IN NO EVENT SHALL SELLER BE LIABLE FOR ANY INDIRECT, SPECIAL, 

INCIDENTAL, OR CONSEQUENTIAL DAMAGES RESULTING FROM 

SELLER’S PERFORMANCE OR FAILURE TO PERFORM HEREUNDER OR 

THE FURNISHING, PERFORMANCE, OR USE OF ANY GOODS OR SER- 

VICES SOLD PURSUANT HERETO. IN NO EVENT SHALL THE AMOIJNT 

OF SELLER’S LIABILITY EXCEED THE AMOUNTS PAYABLE BY BUYER 

HEREUNDER. SELLER EXPRESSLY DISCLAIMS ANY LIABILITY FOR ANY 

WARRANTY, EXPRESS, IMPLIED OR STATUTORY, INCLUDED BUT NOT 

LIMITED TO WARRANTY OF MERCHANTABILITY AND FITNESS FOR A 

PARTICULAR PURPOSE, EXCEPT SUCH WARRANTIES EXPRESSLY IDEN- 

TIFIED AS WARRANTIES AS ARE SET FORTH IN SELLER’S CURRENT 

OPERATING MANUAL, CATALOG OR OTHER WRITTEN GUARANTY 

COVERING THE PRODUCT. 

11 GENERAL 

(a) The sale of goods shall be governed by Uniform Commercial Code and others 

laws of the State of California and Santa Clara County, California shall be the 

appropriate venue and jurisdiction for the resolution of disputes hereunder. 

(b) The Buyer may not assign its rights or obligations under this acknowledgement 

without the prior written consent of Seller, and any purported assignment without 

such consent shall have no force or effect. 

(c) Any waiver by Seller of any default by the Buyer hereunder shall not be deemed to 

be a continuing waiver of such default or a waiver of any other default or any of 

the terms and conditions of this acknowledgement. 

(d) These T & C’s may not be superseded, modified, or amended except in writing 

stating that there is such a modification and signed by an authorized representative 

of each party hereto, provided, however that Seller may modify the specifications 

of the goods sold hereunder if such modification does not change the form, fit, or 

function of such goods. 

(e) This document constitutes the entire terms and conditions agreement between the 

Buyer and Seller with regard to the goods or services purchased or provided and 

expressly supersedes and replaces any prior or contemporaneous agreements, written 

or oral relating to such goods or services. 

(f) In no event shall Buyer commence any action under this contract later than one 

year after the cause of action has accrued.

DISTRIBUTORS 

Argentina 

Omnilab srl 

Virrey Arredondo 2850 

Buenos Aires, 1426 Argentina 

Tel: 54-11-4553-1100 

Fax: 54-11-4555-1584 

Email: info@omnilab.com.ar 

Analytical Technologies S.A. 

Avda. Cordoba 1131 

C1055AAB, Capital Federal 

Buenos Aires, Argentina 

Tel: +54-11-4814-4445 

Fax: +54-11-4814-4445 

Email: info@analytical-tech.com 

Australia 

Biolab Ltd. 

2 Clayton Road 

Clayton, VIC 3168 Australia 

Tel: 61-3-9263-4300 

Fax: 61-3-9562-7953 

Email: info@aus.biolabgroup.com 

Walker Scientifi c 

Unit 2, 5 Ledger Road 

Balcatta, 6021 Western Australia 

Tel: 61-8-9240-5255 

Fax: 61-8-9240-5266 

Email: gwalker@business.ibc.com.au 

A.I. Scientifi c Pty. Ltd. 

10-22 Hornibrook Esplanade 

Clontarf, Qld, 4019 Australia 

Tel: 61-7-3105-5000 

Fax: 61-7-3283-7933 

Email: aimail@aiscientifi c.com 

Website: www.aiscientifi c.com 

Bolivia 

Sistemas Analíticos SRL 

Calle Barachavi 138- Casilla de Correo 

2853-Santa Cruz de la Sierra, Bolivia 

Tel: ++591 3 351 6298 

Fax: ++591 3 311 9209 

Email: cmontes@sistemasanaliticos.com.bo 

Website: www.sistemasanaliticos.com.bo 

Brasil 

Cromatec 

Rua Charles Darwin, 483 

Vila Sta Catarina 

Sao Paul - SP CEP. 04379-060 

Tel: 55-11-5670-2510 

Fax: 55-11-5670-2522 

Email: cromatec@cromatec.com.br 

Website: www.cromatec.com.br 

UNITECH Brasil (offices in DF, RJ, SP, RS) 

Av. Brasilia, 1080 – 2o. Andar 

Paulinia, Sao Paulo, Brasil 13140-000 

Tel: (19) 3833-4062 

Fax: (19) 3874-4841 

Website: www.UNITECHusa.com 

Interprise Instrumentos Analiticos Ltda. 

Rua Maria Das Dores 

Leal de Queiroz, 784 

Paulina, SP 131400-000 Brasil 

Tel: 55-19-3874-7147 

Fax: 55-19-3874-7140 

Agilent Technologies 

Alameda Araguaia, 1142 

Alphaville Barueri 

Sao Paulo, 06455-940 Brasil 

Tel: 55-11-7297-3776 

Fax: 55-11-7297-3798 

Chemetric Instrumentacao Analitica Ltda 

Estr. Dos Bandeirantes 1430/201 

Jacarepagua, Rio de Janeiro 

CEP 22710-113 – RJ, Brasil 

Tel: 55-21-2426-6236 / 6251 

Email: chemetric@chemetric.com.br 

Website: www.chemetric.com.br 

Merck S.A., 

Estrada dos Bandeirantes, 1099 

Cep: 22710-571 

Jacarepaguá 

Rio de Janeiro - RJ - Brasil 

Tel: +55 (21) 2444-2000 

Fax: 2445-2263 

Website: www.merck.com.br 

NOVA ANALÍTICA 

IMPORTAÇÃO E EXPORTAÇÃO LTDA 

Rua Assungui, 432 

04131-000 - São Paulo, SP 

Tel: +55 11 2162 8080 

Fax: + 55 11 2162 8081 

Email: adriene.sandoval@novanalitica.com.br 

Website: www.analiticaweb.com.br 

Chile 

Equilab Ltda. 

San Isidro 1839 

Santiago, Chile 

Tel: 56-2-5569974 

Fax: 56-2-5514006 

Email: ventas2@equilab.cl 

China & Hong Kong Territories 

LWL Development Ltd. 

Room 1920-21, Metro Centre II 

21 Lam Hing Street, Kowloon Bay 

Koowloon, Hong Kong 

Tel: 852-27572332 

Fax: 852-27573811 

Email: lwlltlhk@netvigator.com 

Tegent Technologies Ltd. 

Room 2103-05 Westin Centre 

26 Hung To Road, Kwun Tong 

Kowloon, Hong Kong 

Tel: 852-2759-2182 

Fax: 852-2758-3830 

Email: tegent@netvigator.com 


Asia Pacifi c Service Centre 

CSA for Life Science & Chemical Analysis Group 

Tel: 65-62157607 

Fax: 65-62157925 

Toll-free for China: 

Tel: 800-810-0712 

Fax: 800-810-0717 

Suntek Science Instruments Co., Ltd. 

Suite 501, No. 240 Baidi Road 

Tianjin, China 300192 

Tel: 022-8789-0415 

Fax: 022-8789-0417 

General Manager: Mr. Zhang, Bin-Chen 

Agilent Technologies Shanghai Branch 

12 F Novel Building, No. 887, Huaihai 

Zhong Road, Shanghai, 200020 China 

Tel: 86-21-64456247 

Fax: 86-21-64747708 

Agilent Technologies Hong Kong Branch 

24 F, Cityplaza One, 1111 Kings Road 

Taikoo Shing, Hong Kong 

Tel: 852-652157609 

Fax: 852-652726037 

Colombia 

UNITECH Colombia 

Calle 38, #45-46 

Barranquilla, Colombia 

Tel: +57-5-340-8536 

Fax: +57-5-340-7536 


Casa Cientifi ca 

Carrera 27A 49ª41 

Bogota, Colombia 

Tel: +571-3126310 

Fax: +571-3126304 

Email: casacam@casacientifi ca.com 

Website: www.casacientifi ca.com 

Costa Rica 

Orgoma S.A. 

El Carmen de Paso Anco 

175 Mts al Este, San Jose, Costa Rica 

Tel: 506-226-0906 

Fax: 506-226-0408 

Email: orgoma@racsa.co.cr 

Electronica 

P.O. Box 2733-1000 

Hacienda Vieja Curridabat 

San Jose, Costa Rica 

Tel: 506-272-3700 

Fax: 506-272-0460 

Email: biocien@racsa.co.cr 

UNITECH Costa Rica 

200 metros Norte Y 10 metros Oeste 

del Restaurante La Casona del Pueblo 

San Antonio de Coronado, Casa #123 

San Jose, Costa Rica 

Telefax: +506-294-0768 


Ecuador 

UNITECH Ecuador 

Guerrero Martinez 519 y P.P. Gomez 

Urdaneta, Guayaquil, Ecuador 

Tel: +593-4-236-0976 

Fax: +593-4-237-4435 


European Territories 

LCTech GmbH 

Bahnweg 41 

D-84405 Dorfen, Germany 

Tel: 49(0)-80 81-93 68-0 

Fax: 49(0)-80 81-93 68-10 

Email: info@lctech.de 

Guatemala 

Analitica Quimica 

P.O. 750, Km 15.5 Carretera 

Roosevelt Zona 11, Centro Comercial 

Molino Local 22 

Guatemala Ciudad, Guatemala 

Tel: 502-2-435-5360 

Fax: 502-2-435-4464 

Email: anaqui@guate.net 

IPESA 

9 CALLE 15-45 ZONA 13, Edifi cio Plaza 

Trafalgar 

Guatemala, C. A. 01013 

Tel: +(502)384 8181 

Fax: +(502)384 8111 

Email: recepcion_daq@ipesa.com 

India 

Spinco Biotech PVT LTD 

No. 4 Vaidyaram Street 

T. Nagar, Madras - 600 017 India 

+91 44 2434 01 74 phone 

+91 44 2434 07 61 Fax 

Indonesia 

PT. Perkindo Mitra Analitika 

Komplek Graha Kencana Blok BH 

JI. Raya Pejuangan 88 Kebon 

Jeruk, Jakarta Barat, Indonesia 

Tel: 62-21-5332449 

Fax: 62-21-53673916 

Email: perkindo@cbn.net.id 

Israel 

Manbar Tech 

17A Lazarov Street 

Rishon Le-Zion, 75106 Israel 

Tel: 972-3-9512020 

Fax: 972-3-9511099 

Korea 

Young Lin Instrument Co. Ltd. 

899-6 Hogye 2-dong, Dongan-gu, 

Anyang-si, Kyonggi-do, 431-836 Korea 

Tel: 82-15443744 

Fax: 82-31-4673770 

Interface Engineering Co. Ltd. 

8F, BMS Building, 829, Yeoksam-dong, 

Gangnam-gu, Seoul, Korea, 135-936 

Tel: 82-2-4002605 

Fax: 82-2-4060762 

Email: ckjoo@interface.co.kr 

In Sung Chroma-Tech Co. Ltd. 

In Sung Bldg., 89-106, Shinjung 2-Dong, 

Yangcheon-Ku, Seoul, Korea 

Tel: 82-2-2644-1991 

Fax: 82-2-2644-1996 

Malaysia 

Fisher Scientifi c (M) Sdn Bhd. 

No. 3, Jalan Sepadu 25/123 

Taman Perindustrian Axis, Seksyen 25 

40400 Shah Alam, Darul Ehsan 

Selangor, Malaysia 

Tel: 60-3-5228888 

Fax: 60-3-5218899 

Mexico 

ABC Instrumentación Analítica S.A. de C. V, 

Tigre No.33 Col. Actipan del Valle 

C.P. 03230 México DF. 

Tel: 01 55 5524-2208 

Fax: 01 55 5534-4107 

Website: www.abcia.com.mx 

Email: ventasvap@abcia.com.mx 

Perkin Elmer de Mexico S.A. 

Macedonio Alcal No. 54 

Col. Guadalupe Inn 

Mexico D.F., 01020 Mexico 

Tel: 52-55-56517077 

Fax: 52-55-56607734 

Sintesis y Formulaciones de 

Alta Tecnologia SA de CV 

Olmo No. 32, Col. Valle de Pinos 

54040 Tlainepantla 

Edo. De Mexico, Mexico 

Tel: 52-55-53975008 

Fax: 52-55-53976658 

Email: sifatec@webtelmex.net.mx 

Varian S.A. 

Concepcion Beistegui No. 109 

Col. Del Valle, 3100 Mexico 

Tel: 52-55-5239162 

Fax: 52-55-5239472 

New Zealand 

Alphatech Systems & Co. Ltd. 

P. O. Box 62613 

Ellerslie South, 1131 

Auckland, New Zealand 

Telephone: ++64 9 580 1959 

Fax: ++64 9 580 2044 

Email: sales@alphatech.co.nz 

Medtec Products Ltd. 

Unit C, 8 Vega Place 

Mairangi Bay, Aukland, New Zealand 

Tel: 64-9-479-1068 

Fax: 64-9-479-1450 

Email: customer.service@medtec.co.nz 

Panama 

Promed S.A. 

Urb. Industrial Costa del Este, Calle2da 

Edifi cio Promed, Apartado 6281 

Zona 5, Republic of Panama 

Tel: 507-271-3149 

Fax: 507-271-3164 

Email: segundo@promed.com.pa 

Peru 

Cientifi ca Andina S.A.C. 

Av. Dos De Mayo No. 276 

Mirafl ores, Lima 18, Peru 

Tel: 51-1-444-9983 

Fax: 51-1-444-9937 

Sulabsa Suministros de Laboratorio S.A. 

Av. Javier Prado Este 1570, #301 

Lima 27, Peru 

Tel: 51-1-224-5398 

Fax: 51-1-224-4418 

UNITECH Peru 

Parque San Fernando, 141 

La Perla Alta, Callao, Peru 

Tel: +51-1-420-3804 

Fax: +51-1-420-3660 


Philippines 

DAKILA TRADING CORPORATION 

208 Pilar Street 

Mandaluyong City, 1550 Philippines 

Contact: Mr. Richard Tee 

Tel: (632) 724-75-11 

Fax: (632) 721-07-39 

Email: sales@dakila.com 

107 




10/11

DISTRIBUTORS 

Continued from previous page 

Singapore 


Asia Pacifi c Service Centre 

CSA for Life Science & 

Chemical Analysis Group 

Tel: 65-62157607 

Fax: 65-62157925 

Agilent Technologies Singapore (Sales) Pte Ltd 

Life Sciences and Chemical Analysis 

1 Yishun Avenue 7 

Singapore 768923 

Tel: +65-6377-1688 - Main line 

(ask for LSCA Sales) 

Fax: +65-6822-8247 

Perkin Elmer Asia 

29, International Business Park 

#03-04/06, Acer Bldg, Tower B 

609923 Singapore 

Tel: 65-8962136 

Fax: 65-8962146 

South Africa 

Anatech Instruments 

13 Langwa Street 

Strydompark Randburg, South Africa 

Tel: 27-11-7923300 

Fax: 27-11-7923363 

Email: sales@anatech.co.za 

Chemetrix Pty Ltd. 

Block J, Central Park, 400 16th Road 

Midrand, South Africa 

Tel: 27-11-5419801 

Fax: 27-11-5419802 

Taiwan 

Inpac International Corp. 

12F, No. 79 Sec. 1 Hsin Hai Road 

Taipei, R.O.C. 106 Taiwan 

Tel: 886-2-23637996 

Fax: 886-2-23923975 

Email: inpac@ms10.hinet.net 

Vercotech Inc. 

6F No. 7-1 An Hsing Road, Hsin Tien 

Taipeishien 231, R.O.C. Taiwan 

Tel: 886-2-22113939 

Fax: 886-2-22115522 

Email: vercopak@ms5.hinet.net 

Analab Corporation 

6F-2, No. 181 Sec. 2 Changan East Road 

Taipei, R.O.C. Taiwan 

Tel: 886-227-766931 

Fax: 886-227766932 

Email: analab@ms46.hinet.net 

Abetech Taiwan Inc. 

11F, No. 208 Rueiguang Road 

Taipei, R.O.C. Taiwan 

Tel: 886-2-87512323 

Fax: 886-2-887512020 

Thailand 

Fortune Scientifi c Co, Ltd. 

301/34 M.6 Phaholyothin Road 

Anusawaree, Bangkhen, Bangkok, 

10220 THAILAND 

Tel: 662 986 1250 till 1254 

Fax: 662 986 1255, 662 986 1243 

Email: sales@fortunesci.com 

Website: www.fortunesci.com 

Executive Trading Limited 

55/194-5 Moo 6, Soi Yothin Phattana, Pradit 

Manutham Road, Latphrao 

Bangkok 10230 Thailand 

Tel: 662-5150150 

Fax: 662-5150144 

Email: exec@ji-net.com 

Perkin Elmer Ltd. 

290 (1st fl oor), Raintree Offi ce Garden 

Soi Japanese School, Rama 9 Road 

Khwang Bangkapi, Khet Huay Khwang 

Bangkok, 10320 Thailand 

Tel: 66-2-3197901 

Fax: 66-2-3197900 

Uruguay 

Pablo Ferrando S.A. 

P.O. Box 370, Avda Italia 2877 

Montevideo, 11600 Uruguay 

Tel: 598-2-4872586 

Fax: 598-2-4870131 

Email: ferrando@netgate.com.uy 

ASM 

Gabriel Pereira 2841 Apto. 601 

11300 Montevideo, Uruguay 

Tel / Fax: 598-2-709-6955 

Email: info@asm.com.uy 

US 


Life Sciences/Chemical Analysis Group 

Tel: 800-227-9770 or 302-993-5304 

Fax: 302-633-5301 

Email: cag_sales-na@agilent.com 

Website: www.agilent.com 

4CHROM Inc. 

3015 Barrow Drive 

Raleigh, NC 27616 

Tel: 866-424-7661 or 919-872-5700 

Fax: 919-872-5738 

techsupport@4chrom.com 

Chrom Tech, Inc. 

PO Box 240248 

Apple Valley, MN 55124 Tel: 800-822-5242 

or 952-431-6000 

Fax: 952-431-6345 

Email: sales@chromtech.com 

Website: www.chromtech.com 

Fisher Scientifi c International Inc. 

Customer Service & Sales Support 

Tel: 800-766-7000 

Website: www.fi sherscientifi c.com 

Government Scientifi c Source, Inc. 

8460 Tyco Road 

Vienna, VA 22182 

Tel: 800-248-8030 or 703-734-1805 

Fax: 703-734-1803 

Email: sales@govsci.com 

Website: www.govsci.com 

P.J. Cobert Associates, Inc. 

PO Box 460046 

St. Louis, MO 63146 

Tel: 800-972-4766 or 314-993-2390 

Fax: 314-993-2491 

Email: cobert@cobertassoc.com 

Website: www.cobertassoc.com 

Perkin Elmer Corporation 

710 Bridgeport Avenue 

Shelton, CT 06484-4794 

Tel: 800-762-4000 or 203-925-4600 

Fax: 203-925-4654 

Email: info@perkinelmer.com 

Website: www.perkinelmer.com 

Phanco P.R. 

PO Box 10464 

Granada Hill 1-A 

Ponce, PR 00732 

Tel: 787-844-7371 

Fax: 787-843-0657 

Email: phancopr@hotmail.com 

Sci-Con 

631 Executive Drive 

Winter Park, FL 32789 

Tel: 800-433-6865 or 407-647-4900 

Fax: 407-644-4355 

Email: jimwscicon1@cs.com 

Website: www.scicon.net 

Shimadzu Scientifi c Instruments 

7102 Riverwood Drive 

Columbia, MD 21046 

Tel: 800-477-1227 

Email: webmaster@shimadzu.com 

Website: www.shimadzu.com 

Thompson Instruments 

354 Tyler Rd. 

Clear Brook, VA 22624 

Tel: 540-667-7044 

Fax: 540-667-6878 

Email: info@hplc.com 

Thomson Instrument Company 

1121 South Cleveland Street 

Oceanside, CA 92054 

Tel: 800-541-4792 or 760-757-9182 

Fax: 760-757-9367 

Email: folks@hplc1.com 

Website: www.hplc1.com 

VWR International 

1310 Goshen Parkway 

West Chester, PA 19380 

Tel: 800-932-5000 

Email: solutions@vwr.com 

Website: www.vwr.com 

Venezuela 

Corporacion Cientifi ca Venezolana 

C.A. Comercial 

Libertador Piso 1 Ofc. 1-2 

Caracas, 1070 Venezuela 

Tel: 58-2-127614757 

Fax: 58-2-127623050 

Email: ccvjharb@telcel.net.ve 

Agilent Technologies de Venezuela 

Life Science & Chemical Analysis Group 

Tel: 58-2-122094609 

Fax: 58-2-122094618 

Vietnam 

Tramat Co., Ltd. 

12 Hang Duong Street 

Hanoi, Vietnam 

Contact: Phung Ngoc Phuc 

Email: phuc_phungngoc@tramat.com.vn 

Tel: +84-4-8281631 

Fax: +84-4-9283289 

Offi ce email: info@tramat.com.vn 

Website: http://www.tramat.com.vn 

For all other countries, please contact 

Pickering Laboratories directly.

CATALYST FOR SUCCESS 


1280 Space Park Way / Mountain View, CA 94043 / www.pickeringlabs.com 

sales@pickeringlabs.com / support@pickeringlabs.com 

800-654-3330 / 650-694-6700 / Fax: 650-968-0749

PRODUCT CATALOG - Grupo BioMaster

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