Gene gain and loss: aCGH ISA CGH

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Com p arative Gen om ic Hybrid iz ation ● ● Meth od for an alyz in g gen om ic DNA for u n balan ced gen etic alteration s (CVN) Based on flu orescen t h ybrid iz ation of DNA Techniques ● ● FISH, PCR, Sou th ern A rray <strong>CGH</strong> ● ● ● ● Clon es (BAC, YAC, PAC) PCR n on - red u n d an t Oligonucleotides cDNA
1. Clon es to cover a gen om ic region 2. Extraction an d p u rification 3. Array DNA on to glass slid es Exam p le: Array <strong>CGH</strong> BAC probes 4. Hybrid iz ation of labeled n orm al an d tu m or gen om ic DNA to th e m icroarray 5. An alyz e flu orescen ce ratio
Page 1 and 2: Gene gain and los s : aCGH ISA CGH
Page 3: CNV/ CNP Cop y n u m ber variation
Page 7 and 8: Array CGH Adv antage ● An alysis
Page 9 and 10: ISA CGH ISACGH allows visualiz in g
Page 11 and 12: General outline INPUT: gen e exp re
Page 13 and 14: Genomic copy num ber es tim ation S
Page 15 and 16: Plotting data and copy num ber es t
Page 17 and 18: Minim al com m on regions w ith con
Page 19 and 20: Functional annotation of altered co
Page 21 and 22: An exam ple
Page 23 and 24: 2. CGH array data Ejem plo
Page 25 and 26: 4. Gender labels (optional) Ejem pl
Page 27 and 28: 6. Gene expression data Ejem plo
Page 29 and 30: 8. Scale value (optional) Ejem plo
Page 31 and 32: 9. Reference lines (optional) Ejem
Page 33 and 34: Ejem plo 10. If you have replicates
Page 35 and 36: Ejem plo 11. Choose the representat
Page 37 and 38: Ejem plo 13. Download data form reg
Page 39 and 40: Ejem plo 15. Zoom y functional anal
Page 41 and 42: Ejem plo 15. Zoom y functional anal
Page 43 and 44: http://gepas.bioinfo.cipf.es/cgib

Gene gain and loss: aCGH ISA CGH

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