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RNA Electrophoresis
產 品 目 錄 Product Catalog
生
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電 泳 需 要 藥 品 與 緩 衝 液 (Agarose, Electrophoresis Buffer and Related Serial Products)
RNA EZ-VISION ® Loading Buffer, 1.5X (Cat. no.: N717)
- 只 需 一 個 步 驟 即 可 完 成 樣 品 的 準 備 和 denature
- 使 用 方 法 為 1.5X loading dye 形 式
例 : sample 5uL + 10 uL RNA EZ-VISION loading buffer, 60 度 10 分
鐘 --> loading gel --> run gel --> UV 觀 察
- 靈 敏 度 : 150 ng RNA
- 後 續 可 做 Northern Blotting
Formaldehyde-Free RNA Gel Kit (Cat. No.: N726)
- 完 全 不 含 Formaldehyde 和 EtBr, 可 以 不 用 在 Hood 內 操 作
- 只 需 一 個 步 驟 即 可 完 成 樣 品 的 準 備 和 denature
- 一 套 kit 可 作 約 30 片 50 mL 的 膠 片
- 內 含 : Formaldehyde-Free RNA Gel Running Buffer 10X, 2X500 mL,
Formaldehyde-Free RNA Gel Solution 10X, 150 mL, Formaldehyde-Free
RNA Gel Loading Buffer 2X, 3X2 mL
- 後 續 可 做 Northern Blotting
Comparison between ethidium bromide and RNA EZ-
Vision®. Total RNA was purified from K562 cells with Ribozol
RNA Extraction Reagent. Samples were denatured at
65°C for 10 minutes in RNA EZ-Vision® loading buffer containing
either 0.003% ethidium bromide or RNA EZ-Vision®
dye. The denatured RNA was loaded onto a 2% formaldehyde
denaturing agarose gel (Agarose I, Code: 0710) and
resolved for 1.5 hours at 5.1 V/cm. Bands were visualized
by UV illumination and image capture was performed with
a Syngene GBox-HR Gel Doc System with a SYBR® Green
filter.
Northern blot analysis of PGK1 mRNA resolved on Formaldehyde- Free RNA Gel.
Gel A: 20 μg of total yeast RNA was applied per lane to an agarose Formaldehyde-Free RNA Gel and resolved at 8V/cm.
Bands were visualized on a UV transilluminator.
Gel B: Bands were transferred to Hybond N+ membrane and probed overnight with a radiolabelled oligonucleotide to
PGK1 mRNA.
Gel C: Membranes were stripped and reprobed overnight with an oligonucleotide to SCR1 RNA loading control. Data
provided by Jeff Coller, Ph.D., Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio.
Lane 1: Untransformed control Lane 2: High Copy PGK1 vector Lane 3: Low Copy PGK1 vector Lane 4: High Copy PGK1
vector Lane 5: Low Copy PGK1 vector
Comparison of Formaldehyde -Free RNA dye to ethidium bromide
staining. Total RNA was extracted from K562 cells with Ribozol® RNA
Extraction Reagent (N580). Samples were denatured in Formaldehyde-
Free RNA Loading Buffer containing either ethidium bromide or Formaldehyde-Free
RNA dye and incubated for 10 minutes at 65°C. The
samples were applied to a 2% Formaldehyde-Free RNA Agarose Gel
(Agarose I, Code: 0710) and resolved for 1.5 hours at 5.1 V/cm. Image
capture was
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