綜合目錄PDF 檔下載連結 - 波仕特生物科技股份有限公司

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Custom Service 產品目錄 Product Catalog 客製服務載體構築等分子生物相關服務 (Molecular Cloning and Others) Plasmid DNA Purification (Midiprep & Maxiprep) 部分 : 客戶須知服務範圍客戶提供物品客戶提供資訊 midiprep 給予 plasmid 總量 >100ug OD 260/280 = 1.8 ~ 1.95 Plasmid (>10ng/uL, >10uL) E. coli plate plasmid 資訊 maxiprep 給予 plasmid 總量 >500ug OD 260/280 = 1.8 ~ 1.95 Plasmid (>10ng/uL, >10uL) E. coli plate plasmid 資訊交貨項目服務項目定序結果 Plate Plasmid 報告書 1. Cloning V V V V 2. Sub-Cloning V V V V 3. TA Cloning V V V V 4. Site Direct Mutation V V V V 5. Plasmid DNA Purification Midiprep Maxiprep V V V V 除了以上介紹的服務之外 , 若還有特別的實驗需求也請洽波仕特公司 E-mail: service@bio-protech.com.tw 186 Protech Technology Enterprise Co., LTD www.bio-protech.com.tw
Appendix 產品目錄 Product Catalog 附錄 (Appendix) 附錄 DNA 及蛋白質電泳膠片濃度及分離範圍對照表 % of Agarose (w/v) DNA Separation Range (bp) 0.3 5,000 – 60,000 0.6 1,000 – 20,000 0.8 800 – 10,000 1.0 400 – 8,000 1.2 300 – 7,000 1.5 200 – 4,000 2.0 100 – 3,000 3.0 < 100 % of Acrylamide 19:1 (w/v) DNA Separation Range (bp) 3.5 100 – 1,000 5.0 100 – 500 8.0 60 – 400 12.0 50 – 200 20.0 5 – 100 % of Acrylamide (w/v) 8 50 – 200 10 20 – 100 12 10 – 50 Protein Separation Range (kD) 蛋白質電泳 PAGE gel 配方表 Resolving Gel 16% 15% 14% 13% 12.5% 12% 10% 8% 7.5% dH 2 O (40%, mL) dH 2 O (30%, mL) 3.4 2.07 3.65 2.15 3.9 2.73 4.15 3.07 4.275 3.23 4.4 3.4 4.9 4.07 5.4 4.73 5.52 4.9 4x Lower Gel Buffer (pH8.8)* 2.5 mL 2.5 mL 2.5 mL 2.5 mL 2.5 mL 2.5 mL 2.5 mL 2.5 mL 2.5 mL Acrylamide (40%, mL) Acrylamide (30%, mL) 4 5.33 3.75 5 3.5 4.67 3.25 4.33 3.125 4.17 3 4 2.5 3.33 2 2.67 1.875 2.5 APS (10%) 100 µL 100 µL 100 µL 100 µL 100 µL 100 µL 100 µL 100 µL 100 µL TEMED 4 µL 4 µL 4 µL 4 µL 4 µL 4 µL 4 µL 4 µL 4 µL Total volume 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL Stacking Gel 5% 5% 4% dH 2 O (40%, mL) dH 2 O (30%, mL) 4x Upper Gel Buffer (pH6.8)* Acrylamide (40%, µL) Acrylamide (30%, µL) 3.07 2.862 1.228 1.145 3.195 3.028 1.25 ml 500 µL 1.25 ml 625 833 250 333 500 667 APS (10%) 50 µL 20 µL 50 µL TEMED 5 µL 2 µL 5 µL Total volume 5 ml 2 ml 5 ml * 4X Lower Gel Buffer (pH8.8) 及 4X Upper Gel Buffer (pH6.8) : 內含 Tris buffer、SDS 等成份 , 詳細訂購資訊請見目錄頁 , 蛋白質電泳相關產品介紹 * Acrylamide 可使用 37.5:1 或 29:1 塑膠耗材使用注意事項 — 滅菌及離心條件 PCR Tubes PCR Plates Assay& Deep Well Plates Microcentrifuge Tubes Screw Cap Tubes 最大離心力 (RCF, g) 4,000 4,000 4,000 14,000*—20,000 20,000 滅菌條件 121°C, 15 分鐘 , 15psi/1 atm. 滅菌釜降溫至 80 °C 前請勿取出 , 以免產品擠壓變形 * microcentrifuge Tubes 內裝 phenol/chloroform 時 , 最大離心力請勿超過 14,000 g Protech Technology Enterprise Co., LTD www.bio-protech.com.tw 187
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我們的服務宗旨 Protech
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目錄 CONTENTS 生化藥品
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目錄 CONTENTS 實驗儀器
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Bio-chemicals 產品目錄 Produ
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DNA Electrophoresis 產品目錄
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RNA Electrophoresis 產品目錄
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Protein Electrophoresis 產品目
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Protein Electrophoresis 產品目
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介面活性劑 (Detergents)
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Detergents 產品目錄 Product
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Bacterial Culture Medium / Antibiot
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Bio-Enzyme 產品目錄 Product
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Histology 產品目錄 Product C
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Protein Purification and Analysis
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蛋白質表現系統 (Protei
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Protein Expression System 產品
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核酸純化 (DNA/RNA Purificati
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DNA/RNA Purification 產品目
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PCR/qPCR/Cloning 產品目錄 Pr
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Pathogen Diagnostics 產品目
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Cell Culture 產品目錄 Produc
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實驗耗材波仕特提供
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Plastic Consumable 產品目錄
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微量離心管 (Microtubes & T
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Page 142 and 143: Plastic Consumable 產品目錄
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Page 196: 2013
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綜合目錄PDF 檔下載連結 - 波仕特生物科技股份有限公司

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