綜合目錄PDF 檔下載連結 - 波仕特生物科技股份有限公司
綜合目錄PDF 檔下載連結 - 波仕特生物科技股份有限公司
綜合目錄PDF 檔下載連結 - 波仕特生物科技股份有限公司
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PCR/qPCR/Cloning<br />
產 品 目 錄 Product Catalog<br />
Other Enzymes<br />
Polymerase Applications Proofreading<br />
Strand displacement<br />
Error rate<br />
Thermal<br />
stability<br />
Extend from<br />
RNA primer<br />
Extend<br />
from nick<br />
phi29 polymerase<br />
M-MuLV reverse transcriptase<br />
T7 RNA polymerase<br />
T7 DNA polymerase<br />
Sequencing<br />
cDNA synthesis<br />
RT-PCR<br />
Transcription<br />
Gene expression<br />
Site-directed mutagenesis<br />
Sequencing<br />
3’→5’<br />
Strong<br />
Very strong N/A - V V<br />
- Strong N/A - N/A N/A<br />
- N/A N/A - N/A N/A<br />
3’→5’<br />
Very strong<br />
- 15 X 10 -6 - V -<br />
Terminal transferase 3’ Labeling - - N/A N/A N/A N/A<br />
Bst DNA polymerase<br />
DNA polymerase I large<br />
(Klenow), exonuclease<br />
T4 DNA polymerase<br />
T4 DNA polymerase,<br />
exonuclease minus<br />
Sequencing<br />
Cloning<br />
Labeling<br />
Cloning<br />
Fill-in<br />
Mutagenesis<br />
Cloning<br />
- Very strong N/A V N/A N/A<br />
3’→5’<br />
Strong<br />
3’→5’<br />
Very strong<br />
Strong 100 X 10 -6 - V V<br />
N/A Low - V -<br />
Sequencing - N/A Low - V -<br />
相<br />
關<br />
產<br />
品<br />
Ligasae<br />
Applications<br />
Ligate cohesive<br />
ends<br />
Ligate blunt<br />
ends<br />
Nicks in<br />
dsDNA<br />
DNA-RNA<br />
heteroduplex<br />
formation<br />
RNA 3’ labeling<br />
ssRNA and<br />
dsDNA oligo<br />
joining<br />
T4 DNA ligase, low<br />
concentration<br />
T4 DNA ligase, high<br />
concentration, rapid<br />
kit<br />
T4 RNA ligase<br />
Cloning<br />
Linker addition<br />
Cloning<br />
Linker addition<br />
RNA/DNA joining<br />
3’ labeling<br />
V V V V N/A N/A<br />
V V V V N/A N/A<br />
N/A N/A N/A N/A V V<br />
DNA compatibility<br />
Nuclease<br />
Polarity Substrate<br />
Activity w/o<br />
5’-phosphate<br />
3’ Recessed<br />
ends<br />
3’ Protruding<br />
ends<br />
Blunt<br />
ends<br />
Nicks<br />
Partial ss<br />
extension<br />
digestion<br />
Product<br />
Phosphorothioate<br />
cleavage<br />
phi29 polymerase<br />
M-MuLV reverse<br />
transcriptase<br />
T7 RNA polymerase<br />
5’→3’<br />
ssDNA<br />
dsDNA<br />
+/- 1 +/- V V - 3’<br />
ssDNA<br />
dNMP<br />
3’→5’ ssDNA V - +/- +/- N/A N/A dNMP 3 -<br />
3’→5’<br />
ssDNA<br />
dsDNA<br />
V V +/- 2 +/- 2 V 5’<br />
N/A ssRNA N/A N/A N/A N/A N/A N/A<br />
ssDNA<br />
dNMP<br />
T7 DNA polymerase<br />
Ribonucleotide<br />
s<br />
-<br />
-<br />
N/A<br />
1 = Strongly prefers 5’-P, but will degrade w/o it.<br />
2 = Exo III can't blunt a 3’ extension.<br />
3 = Exo I can’t cleave the final bond, yielding dNMP’s and a dinucleotide.<br />
Protech Technology Enterprise Co., LTD<br />
www.bio-protech.com.tw<br />
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