Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
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What sort <strong>of</strong> molecules are fluorescent?<br />
1. Endogenous organic fluorophores<br />
Most common aut<strong>of</strong>luorescent molecules:<br />
Flavins, NADH, NADPH, elastin, collagen, lip<strong>of</strong>uscin<br />
Avoiding aut<strong>of</strong>luorescence:<br />
choose dye emitting in red with big Stokes shift<br />
add quencher (Crystal violet)<br />
add reducing agent to react with aut<strong>of</strong>luorescent molecules<br />
time-gate fluorescence<br />
7
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