Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
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Correlated Light Microscopy/<br />
Electron Microscopy<br />
MiniSOG (Shu, Tsien PLoS Biol 2011)<br />
Light causes generation <strong>of</strong> singlet oxygen-> DAB polymerized-> binds OsO 4<br />
106 aa monomer, engineered from Arabidopsis LOV domain<br />
tested in cell-lines, worms <strong>and</strong> transgenic mice<br />
Overcomes trade-<strong>of</strong>f between thorough fixation <strong>and</strong> penetration <strong>of</strong> labeling reagent<br />
(Also see APEX protein tag for DAB for EM staining, JD Martell et al. Nat Biotech 2012)
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