Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
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Not all energy emitted as fluorescence<br />
Fluorescence<br />
(10 -9 s)<br />
Absorption<br />
(10 -15 s)<br />
FRET<br />
Internal<br />
conversion<br />
Triplet state<br />
Phosphorescence<br />
(10 2 - 10 -2 s)<br />
Quantum yield = no. <strong>of</strong> fluorescent photons emitted<br />
no. <strong>of</strong> photons absorbed<br />
e.g. EGFP QY=0.6 For every 10 photons absorbed, 6 are emitted.<br />
(at optimal temp, pH etc.)<br />
3
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