Fluorescent Dyes and Proteins - Department of Biochemistry

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Overview 1. What kind of structures are fluorescent 2. How to make and target fluorescent probes 3. Fluorescent probes for cellular structure and function 4. Using light to control cells 22
Putting the signal in context: nuclear labelling (follow DNA even when nucleus breaks down) Fixed cells: Intercalate into DNA DAPI (well away from other channels) Hoechst 33342 Live cells: histone H2B-GFP 23
Page 1 and 2: Fluorescent Dyes and Proteins Mark
Page 3 and 4: Not all energy emitted as fluoresce
Page 5 and 6: What sort of molecules are fluoresc
Page 7 and 8: What sort of molecules are fluoresc
Page 9 and 10: How good is a fluorophore? Green dy
Page 11 and 12: Alexa Fluor Dyes - Photostability L
Page 13 and 14: Antifade Reagents for Live Cells HO
Page 15 and 16: Multiplexing- four main colours Exc
Page 17 and 18: Major bottleneck to using new probe
Page 19 and 20: Not just antibodies for targeting O
Page 21: Site-specific protein labelling met
Page 25 and 26: Mitochondria Fixed cells: anti-cyto
Page 27 and 28: Lipid Rafts BODIPY® FL C 5 -gangli
Page 29 and 30: Cytosol Live cells: CellTracker Gre
Page 31 and 32: The breakthrough of fluorescent pro
Page 33 and 34: Chromophores in switching Green Flu
Page 35 and 36: Small molecule fluorescent sensors
Page 37 and 38: How good is a fluorescent protein?
Page 39 and 40: You MUST worry about FP multimeriza
Page 41 and 42: Fluorescent RNA imaging See single
Page 43 and 44: Why use light to control biology? L
Page 45 and 46: LOV domains react and switch confor
Page 47 and 48: Correlated Light Microscopy/ Electr
Page 49: References Fluorescence probes Mole

Fluorescent Dyes and Proteins - Department of Biochemistry

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