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Fluorescent Dyes and Proteins - Department of Biochemistry

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Site-specific protein labelling methods<br />

1. Binding domain<br />

SNAP-tag (NEB), HaloTag (Promega)<br />

2. Binding peptide<br />

FlAsH (Invitrogen)<br />

3. Enzymatic ligation to peptide<br />

PRIME<br />

AY Ting PNAS 2010<br />

Chen & Ting, Curr. Opin. Biotech. 2005<br />

21

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