Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
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How to dye: it is easy<br />
Multiple ways to modify proteins<br />
(see Molecular Probes catalogue)<br />
Most common ways are to modify:<br />
1. Lysine<br />
or<br />
Protein target<br />
NHS-dye Amide bond<br />
to dye<br />
2. Cysteine<br />
maleimide-dye Thioether bond<br />
to dye<br />
A Add dye to protein for 3 hr<br />
B 1cm Sephadex column to remove most free dye (10 min)<br />
C Dialyse away rest <strong>of</strong> free dye (24 hr)<br />
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