Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
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Antibodies for cellular imaging<br />
Live cells<br />
Label plasma membrane <strong>and</strong> secretory pathway<br />
Penetrate plasma membrane<br />
(microinjection, electroporation, pinosome lysis, streptolysin,<br />
cell permeable peptides, ester cage)<br />
Get dynamics, avoid fixation artifacts<br />
Fixed cells<br />
Permeabilise<br />
Still can give enormous amount <strong>of</strong> useful information<br />
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