Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
Fluorescent Dyes and Proteins - Department of Biochemistry
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Antifade Reagents for Live Cells<br />
HO<br />
H 3<br />
C<br />
Trolox<br />
CH 3<br />
O<br />
C OH<br />
O CH 3<br />
CH 3<br />
-Trolox<br />
+Trolox<br />
Blinking <strong>of</strong> single molecule <strong>of</strong> Atto647N on DNA,<br />
Vogelsang Tinnefeld Ang Chem 2008<br />
• Trolox is an antioxidant that<br />
can reduce bleaching<br />
compatible with live specimens<br />
water-soluble<br />
working conc. ~100 µM<br />
• Ascorbic acid is an alternative<br />
antioxidant<br />
• Depleting oxygen (especially<br />
used for some single molecule<br />
experiments) with Glucose<br />
Oxidase <strong>and</strong> Catalase greatly<br />
reduces bleaching.<br />
• Can stop not only bleaching<br />
but also blinking<br />
13
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