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UPM GEARED UP TOWARDS SERVICING A ... - World Halal Week

UPM GEARED UP TOWARDS SERVICING A ... - World Halal Week

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Real-time PCR<br />

Specificity test on pork primer designed against other meat species (beef and chicken)<br />

• Specificity of the pork-specific primers set designed was tested.<br />

• Only pork sample showed amplification while chicken, beef and no template control<br />

(NTC) did not exhibit any amplification.<br />

• Combination of the primers designed and real-time PCR method optimized gave a<br />

Ct value of 21.58 for pork samples.<br />

• No amplification was detected in the other two samples.

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