Image Capture - NCI Division of Cancer Treatment and Diagnosis ...
Image Capture - NCI Division of Cancer Treatment and Diagnosis ... Image Capture - NCI Division of Cancer Treatment and Diagnosis ...
DCTD Standard Operating Procedures (SOP) Title: Image Capture of Tumor Biopsy Slides from γH2AX Immunofluorescence Assay Page 22 of 25 Doc. #: SOP340533 Revision: D Effective Date: 9/22/2013 APPENDIX 5: FIELD SELECTION FOR IMAGING 1. H&E-stained needle biopsy Below is an H&E-stained needle biopsy illustrating field selection for image capture. Phase-dense regions of the biopsy (lacking necrotic areas, large gaps in the tissue, or areas where tissue has folded onto itself) should be selected at 200x under phase contrast (see Section 2), and then imaged under fluorescence following SOP Section 7.3.8.
DCTD Standard Operating Procedures (SOP) Title: Image Capture of Tumor Biopsy Slides from γH2AX Immunofluorescence Assay Page 23 of 25 Doc. #: SOP340533 Revision: D Effective Date: 9/22/2013 2. Sample phase-contrast and composite fluorescent images 3. Sample DAPI monochrome images and corresponding composite fluorescent images
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DCTD St<strong>and</strong>ard Operating Procedures (SOP)<br />
Title: <strong>Image</strong> <strong>Capture</strong> <strong>of</strong> Tumor Biopsy Slides from γH2AX Immun<strong>of</strong>luorescence Assay Page 22 <strong>of</strong> 25<br />
Doc. #: SOP340533 Revision: D Effective Date: 9/22/2013<br />
APPENDIX 5: FIELD SELECTION FOR IMAGING<br />
1. H&E-stained needle biopsy<br />
Below is an H&E-stained needle biopsy illustrating field selection for image capture. Phase-dense<br />
regions <strong>of</strong> the biopsy (lacking necrotic areas, large gaps in the tissue, or areas where tissue has folded<br />
onto itself) should be selected at 200x under phase contrast (see Section 2), <strong>and</strong> then imaged under<br />
fluorescence following SOP Section 7.3.8.
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