Rezumatul tezei de doctorat - USAMV Cluj-Napoca

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isexualis (761 bp, a identity of 99% and has 7 point mutations compared to the matrix species from GeneBank). 16. The bigger losses caused by identified species (Saprolegnia ferax and Achlya bisexualis), at the cyprinid species from Romania, are observed in the reproduction period, when the percentage of average losses at spawns reaches 41,10%, and 16,70% at larval period. 17. The identified species are causing losses in the cyprinid breeding fishponds from Romania, and at the young fish (4,90%) and adults (with an average of 6,30% in wintertime, 4,60% in springtime, 4,30% in summertime and 4,10% in autumn). 18. The losses caused by saprolegniasis differ from one season and fishery to another, depending on the water physical, chemical and biological conditions, on the breeding technology severity and on the general meteorological conditions. Based on the obtained results, we recommend: 9. We recommend for the analyses of Saprolegnia presence in fishponds, that the water samples should be collected from the 4 lake sides and centre, and from the depth of about 50 cm. 10. For the growth of different Saprolegnia strains, can be used solid culture media (PDA and SGA), but we recommend PDA culture medium for the colonies more rapid growing time. 11. Because, in the DNA analyses there are needed big quantities of fungi, and making allowance for Saprolegnia development mode in the two culture liquid media, we recommend the use of Potato Dextrose Broth (PDB) liquid medium, in the experiments on the genus species. 12. We recommend the fungal DNA extraction with kits (QIAGEN) and avoiding the solutions extraction (PBS and NE), which in spite of the fact that realizes normal DNA purities and quantities, the amplification of Saprolegnia DNA samples produces partialy or was absent. 74
13. In researches on the aquatic fungi we recommend the use of RsaI enzyme, which has a very good specificity for Saprolegnia and Achlya restriction sites, and to avoid AluI and HindIII enzymes, which do not have any specificity for the restriction sites. 14. For the genetic characterization of Saprolegniaceae family aquatic fungi, we recommend the use of PCR-RFLP method, described by Ristaino and al., 1998, adapted by us. 15. To be able to identify precisely the genera and species from Saprolegniaceae family, we recommend the use of DNA sequencing and the results comparison with the ones from GeneBank. 16. To avoid the losses caused by saprolegniasis, in the cyprinid fisheries from centern and north-western part of Romania, we recommend a special attention of the fishery owners in: the permanent water quality control, the vegetation type control, avoiding the fish traumatisms during manipulations, avoiding the stress caused by water level oscillations, and by the forage type used. 75
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UNIVERSITATEA DE ŞTIINłE AGRICOLE
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II.6. REZULTATELE RESTRICłIEI ENZI
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diverse specii piscicole. De aici s
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Fig.1. LocaŃiile în care s-au efe
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A fost constituit un număr de 207
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Sabouraud Dextrose Broth (SDB-bulio
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Echipament, materiale şi reactivi
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Primerii folosiŃi de către noi î
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CAPITOLUL II REZULTATELE CERCETĂRI
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Mediile diametrului coloniilor de S
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laborator specifice metodologiei de
Page 23 and 24: Deoarece am utilizat în experiment
Page 25 and 26: fragmente: primul la aproximativ 45
Page 27 and 28: II.7. REZULTATE PRIVIND STUDIUL ÎN
Page 29 and 30: mai mare genetică, indivizii despr
Page 31 and 32: MenŃionăm faptul că în toate ba
Page 33 and 34: CAPITOLUL III CONCLUZII ŞI RECOMAN
Page 35 and 36: isexualis (761 pb, o identitate de
Page 37 and 38: BIBLIOGRAFIE SELECTIVĂ 1. Colao Ma
Page 39 and 40: 19. Oroian, T.E., 2006, SelecŃia a
Page 41 and 42: UNIVERSITY OF AGRICULTURAL SCIENCES
Page 43 and 44: SPECIES FROM SAPROLEGNIACEAE FAMILY
Page 45 and 46: The specific literature presents up
Page 47 and 48: Fig.1. The locations where the rese
Page 49 and 50: estimation of the spawns infestatio
Page 51 and 52: pellets were filtred, washed with d
Page 53 and 54: The DNA rapid extraction using PBS
Page 55 and 56: We used a comparatively mixture of
Page 57 and 58: II.1. THE RESULTS OF SAPROLEGNIA CU
Page 59 and 60: Reading time 24 48 72 n Culture med
Page 61 and 62: asexual one. We can precisely concl
Page 63 and 64: 1 2 3 4 5 6 7 8 9 700pb- Fig.3. Ele
Page 65 and 66: pairs length. The sequencing result
Page 67 and 68: II.7. RESULTS REGARDING THE GENETIC
Page 69 and 70: higher genetic distance, the indivi
Page 71 and 72: We mention the fact that in al the
Page 73: extraction have values between 1,10
Page 77 and 78: 40. Frisvad, J.C., Bridge, P.D., Ar
Page 79: 61. *** http://www.sigmaaldrich.com
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