Rezumatul tezei de doctorat - USAMV Cluj-Napoca

isexualis (761 bp, a identity of 99% and has 7 point mutations compared to the
matrix species from GeneBank).
16. The bigger losses caused by identified species (Saprolegnia ferax and Achlya
bisexualis), at the cyprinid species from Romania, are observed in the reproduction
period, when the percentage of average losses at spawns reaches 41,10%, and 16,70%
at larval period.
17. The identified species are causing losses in the cyprinid breeding fishponds from
Romania, and at the young fish (4,90%) and adults (with an average of 6,30% in
wintertime, 4,60% in springtime, 4,30% in summertime and 4,10% in autumn).
18. The losses caused by saprolegniasis differ from one season and fishery to another,
depending on the water physical, chemical and biological conditions, on the breeding
technology severity and on the general meteorological conditions.
Based on the obtained results, we recommend:
9. We recommend for the analyses of Saprolegnia presence in fishponds, that the water
samples should be collected from the 4 lake sides and centre, and from the depth of
about 50 cm.
10. For the growth of different Saprolegnia strains, can be used solid culture media (PDA
and SGA), but we recommend PDA culture medium for the colonies more rapid
growing time.
11. Because, in the DNA analyses there are needed big quantities of fungi, and making
allowance for Saprolegnia development mode in the two culture liquid media, we
recommend the use of Potato Dextrose Broth (PDB) liquid medium, in the
experiments on the genus species.
12. We recommend the fungal DNA extraction with kits (QIAGEN) and avoiding the
solutions extraction (PBS and NE), which in spite of the fact that realizes normal
DNA purities and quantities, the amplification of Saprolegnia DNA samples
produces partialy or was absent.
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