Rezumatul tezei de doctorat - USAMV Cluj-Napoca

extraction have values between 1,10 – 1,51, with an average of 1,394, at 260/280 nm
ripple length, and the DNA quantities obtained by the same method, varied between
5,23 – 86,56 ng/µl, with an average of 30,653 ng/µl.
8. In the case of DNA samples extracted with kit QIAGEN, in all the situations the
amplification produced with both primer pairs used, with the visualization of 743 bp
fragments at Saprolegnia ferax, and of 761 bp at Achlya bisexualis.
9. In the case of the DNA samples extracted with PBS solutions, in spite of the normal
DNA purities and quantities, the amplification of Saprolegnia DNA samples, with the
two primer pairs, produced differently, because of the presence of possible fungal
inhibitors, which do not allow the attachment of ITS1 and ITS4 primer pair, and in
the case of using a PCR purification kit, the amplification performed.
10. In the case of the samples extracted with NE solutions, in spite of the normal purities
and quantities, the amplification of Saprolegnia DNA samples, with the two primer
pairs didn’t produced.
11. RsaI enzyme has a very good specificity for Saprolegnia and Achlya restriction sites,
so we opted for its usage in all the fragment restriction tests.
12. AluI and HindIII enzymes, tested by us in the experiment, do not have any specificity
for Saprolegnia and Achlya restriction sites, failing to produce any restricion of the
DNA fragments.
13. In the case of the 10 fisheries and 69 samples analyzed, there are visualized two DNA
length polymorphisms, in the gels.
14. The study of phylogenetic tree, using linkage and Euclidean distances, allows us to
conclude the fact that in the studied fisheries and fishponds, there are different genera
of fungi from Saprolegniaceae family, which have different pathogenicity upon
cyprinid species.
15. After the DNA sequencing, there were identified two species of aquatic fungi, in the
studied fishponds, represented by Saprolegnia ferax (743 bp, a identity of 99% and
has two point mutations compared to the matrix species from GeneBank) and Achlya
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