Rezumatul tezei de doctorat - USAMV Cluj-Napoca

higher genetic distance, the individuals broked from the same branch, are evolving today in
the fishpond 9 from Adrian fishery (var.5) and in the fishponds 8 and 10, from Cefa fishery
(var.12).
II.8. SEQUENCING RESULTS OF THE SAPROLEGNIACEAE FAMILY
FUNGAL SPECIES FROM THE STUDIED LOCATIONS
After the automatic sequencing, performed with ABI Prism sequencer, by
Mycrosinth (Switzerland), there were identified two aquatic fungal species, in the studied
fishponds, represented by Saprolegnia ferax and Achlya bisexualis.
In the case of Saprolegnia ferax species sequencing, the fragment sequenced with
the primers ITS1(forward) and ITS4 (reverse), has a length of 743 bp, a identity of 99% and
has two point mutations compared to the matrix species from GeneBank. At 31 position,
there is observed a deletion between A-C nucleotides, of a C nucleotide, and at 368 position,
there is a substitution of A with C.
After sequencing Achlya bisexualis species, the fragment sequenced with the
primers ITS1(forward) and ITS4 (reverse), has a length of 761 bp, a identity of 99% and has
7 point mutations compared to the matrix species from GeneBank. At 112 position, there is
rematked a substitution of C with T; at 119 position, a substitution of G with A; at 194
position, a substitution of T with G; at 370 and 371 positions, two substitutions of C with A;
at 416 position, a substitution of A with T, and at 633 position, a substitution of A with G.
II.9. THE INCIDENCE OF SAPROLEGNIASIS IN THE CENTRAL
AND NORTH-WESTERN ROMANIAN AREAL
Saprolegniasis is the ”silent killer” of the aquatic species, the disease producing
extremely important losses in the fisheries, particularly in the cyprinid ones.
Saprolegniasis is one of the most important causes of economic losses in aquaculture,
fungal infections are second only to bacterial diseases in economic importance. Fungal
infections are generally restricted to chronic, steady losses. In Japan, there is an annual
mortality rate of 50% in coho salmon (Oncorhynchus kisutch) due to Saprolegnia parasitica
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