Rezumatul tezei de doctorat - USAMV Cluj-Napoca

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150 bp. The electrophoretic profiles are belonging to Saprolegnia ferax species, ulterior sequenced. In the fishpond 4, the fragment length differs as it follows: the first fragment has 500 bp, the second one has about 210-220 bp, and the third one has 150 bp, involving a polymorphism of another fungal species (Achlya bisexualis). In the case of the fungal species from Saprolegniaceae family, collected from Cefa fishery, Bihor county, with 10 fishponds, in the fishponds 1,2,4,5,6,7 and 9 , the 3 fragments have the following lengths: the first fragment 400 bp, the second fragment 200 bp and the third fragment between 130 and 150 bp. Those polymorphisms are belonging to Saprolegnia ferax species. In the fishponds 3,8 and 10, the fragments have different lengths, the sequencing confirming the presence of Achlya bisexualis species. The first fragment is visualized more clearly, having about 450 bp. At the samples originating from the fishponds 8 and 10, it is observed an extra fragment the second one, of 250 bp, the third one having 200 bp and the fourth one about 130 bp, have the same length with the second and third fragments from the other fishpond samples. At Ineu fishery, Arad county, the fragments of all the fungal samples from Saprolegniaceae family, collected from 7 fishponds analyzed, had the same lengths: the first one had 400 bp, the second one 200 bp, and the third one between 130 and 150 bp. The ulterior sequencing analyses confirmed the presence of Saprolegnia ferax species, in this location fishponds. 66
II.7. RESULTS REGARDING THE GENETIC PHYLOGENY STUDY OF SAPROLEGNIACEAE FAMILY FUNGI Tree Diagram for 12 Variables Single Linkage Eucli<strong>de</strong>an distances Var1 Var7 Var3 Var6 Var2 Var8 Var10 Var11 Var4 Var9 Var5 Var12 0 10 20 30 40 50 60 Linkage Distance Fig.4. Phylogenetic analysis of 69 DNA samples from Saprolegniaceae family fungi, isolated from 10 locations Variant 1: Ariniş, with the samples from 1-5, 8,9 fishponds; Motiş, with the samples from the 7 fishponds; Variant 2: Ariniş, with the samples from 6 şi 7 fishponds; Variant 3: Adrian, with the samples from 1–4, 6-8, 10 fishponds; Variant 4: Adrian, with the samples from fishpond 5; Variant 5:Adrian, with the samples from fishpond 9; Variant 6: łaga, with the samples from all the 5 fishponds; Variant 7: Ciurila, with the samples from 1-3, 5-6 fishponds; Daia, with the samples from 1-4 fishponds; Iernut, with the samples from 1-3, 5 fishponds; Cefa, with the samples from 1-2, 4-7, 9 fishponds; Ineu, with the samples from all the 7 fishponds; Variant 8: Ciurila, with the samples from fishpond 4; Variant 9: Chiochiş, with the samples from 1-3 and 5 fishponds; Variant 10: Chiochiş, with the samples from fishpond 4; Variant 11: Iernut, with the samples from fishpond 4; Daia, with the samples from fishpond 5; Variant 12: Cefa, with the samples from 8 and 10 fishponds. 67
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UNIVERSITATEA DE ŞTIINłE AGRICOLE
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II.6. REZULTATELE RESTRICłIEI ENZI
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diverse specii piscicole. De aici s
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Fig.1. LocaŃiile în care s-au efe
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A fost constituit un număr de 207
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Sabouraud Dextrose Broth (SDB-bulio
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Echipament, materiale şi reactivi
Page 15 and 16: Primerii folosiŃi de către noi î
Page 17 and 18: CAPITOLUL II REZULTATELE CERCETĂRI
Page 19 and 20: Mediile diametrului coloniilor de S
Page 21 and 22: laborator specifice metodologiei de
Page 23 and 24: Deoarece am utilizat în experiment
Page 25 and 26: fragmente: primul la aproximativ 45
Page 27 and 28: II.7. REZULTATE PRIVIND STUDIUL ÎN
Page 29 and 30: mai mare genetică, indivizii despr
Page 31 and 32: MenŃionăm faptul că în toate ba
Page 33 and 34: CAPITOLUL III CONCLUZII ŞI RECOMAN
Page 35 and 36: isexualis (761 pb, o identitate de
Page 37 and 38: BIBLIOGRAFIE SELECTIVĂ 1. Colao Ma
Page 39 and 40: 19. Oroian, T.E., 2006, SelecŃia a
Page 41 and 42: UNIVERSITY OF AGRICULTURAL SCIENCES
Page 43 and 44: SPECIES FROM SAPROLEGNIACEAE FAMILY
Page 45 and 46: The specific literature presents up
Page 47 and 48: Fig.1. The locations where the rese
Page 49 and 50: estimation of the spawns infestatio
Page 51 and 52: pellets were filtred, washed with d
Page 53 and 54: The DNA rapid extraction using PBS
Page 55 and 56: We used a comparatively mixture of
Page 57 and 58: II.1. THE RESULTS OF SAPROLEGNIA CU
Page 59 and 60: Reading time 24 48 72 n Culture med
Page 61 and 62: asexual one. We can precisely concl
Page 63 and 64: 1 2 3 4 5 6 7 8 9 700pb- Fig.3. Ele
Page 65: pairs length. The sequencing result
Page 69 and 70: higher genetic distance, the indivi
Page 71 and 72: We mention the fact that in al the
Page 73 and 74: extraction have values between 1,10
Page 75 and 76: 13. In researches on the aquatic fu
Page 77 and 78: 40. Frisvad, J.C., Bridge, P.D., Ar
Page 79: 61. *** http://www.sigmaaldrich.com
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