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Rezumatul tezei de doctorat - USAMV Cluj-Napoca

Rezumatul tezei de doctorat - USAMV Cluj-Napoca

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150 bp. The electrophoretic profiles are belonging to Saprolegnia ferax species, ulterior<br />

sequenced. In the fishpond 4, the fragment length differs as it follows: the first fragment has<br />

500 bp, the second one has about 210-220 bp, and the third one has 150 bp, involving a<br />

polymorphism of another fungal species (Achlya bisexualis).<br />

In the case of the fungal species from Saprolegniaceae family, collected from Cefa<br />

fishery, Bihor county, with 10 fishponds, in the fishponds 1,2,4,5,6,7 and 9 , the 3 fragments<br />

have the following lengths: the first fragment 400 bp, the second fragment 200 bp and the<br />

third fragment between 130 and 150 bp. Those polymorphisms are belonging to Saprolegnia<br />

ferax species. In the fishponds 3,8 and 10, the fragments have different lengths, the<br />

sequencing confirming the presence of Achlya bisexualis species. The first fragment is<br />

visualized more clearly, having about 450 bp. At the samples originating from the fishponds<br />

8 and 10, it is observed an extra fragment the second one, of 250 bp, the third one having<br />

200 bp and the fourth one about 130 bp, have the same length with the second and third<br />

fragments from the other fishpond samples.<br />

At Ineu fishery, Arad county, the fragments of all the fungal samples from<br />

Saprolegniaceae family, collected from 7 fishponds analyzed, had the same lengths: the first<br />

one had 400 bp, the second one 200 bp, and the third one between 130 and 150 bp. The<br />

ulterior sequencing analyses confirmed the presence of Saprolegnia ferax species, in this<br />

location fishponds.<br />

66

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