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Rezumatul tezei de doctorat - USAMV Cluj-Napoca

Rezumatul tezei de doctorat - USAMV Cluj-Napoca

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pairs length. The sequencing results indicated the fact that all the electrophoretic profiles<br />

belonged to Saprolegnia ferax species.<br />

At the fungal species from Saprolegniaceae family, collected from Ciurila fishery,<br />

<strong>Cluj</strong> county, with 6 fishponds, in the fishponds 1,2,3,5 and 6 there can be observed 3<br />

fragments of restricted DNA, the first one is about 400 bp, the second one has 200 bp, and<br />

the third one has 130-150 bp. In the fishpond 4 there can be seen 4 DNA fragments, the first<br />

one at 500 bp, the second one at 250 bp, the third one at 200 bp and the fouth one between<br />

130-150 bp, which indicate the presence of a polymorphism, confirmed by sequencing as<br />

belonging to Achlya bisexualis species.<br />

At the fungal species from Saprolegniaceae family, collected from Chiochiş fishery,<br />

BistriŃa-Năsăud county, with 5 fishponds, in the 1,2,3 and 5 fishponds, can be observed 3<br />

fragments, with the following lengths: the first one between 400 bp and 500 bp, the second<br />

fragment between 200 bp and 250 bp, and the third one between 150-200 bp. Those<br />

electrophoretic profiles, correlated with the sequencing data and the GeneBank comparison,<br />

indicated the presence of Saprolegnia ferax species. In the fishpond 4, the 3 fragments have<br />

different lengths: the first fragment has 500 bp, the second one has about 250 bp and the<br />

third one has 150-200 bp. After the fragment length analyses by electrophoresis, in the<br />

fishpond 4 there is observed a different polymorphism compared to the other fungal DNA<br />

samples, which indicates the presence of another species, confirmed as Achlya bisexualis.<br />

At the fungal species from Saprolegniaceae family, collected from Daia fishery, Alba<br />

county, with 5 fishponds, in the 1,2,3,4 fishponds there is remarked the presence of 3<br />

restriction lanes, the first one having 400 bp, the second one 200 bp, and the third one 130-<br />

150 bp, after the sequencing analyses being i<strong>de</strong>ntified the aquatic fungal species named<br />

Saprolegnia ferax. It can be seen a fragment length polymorphism at the fungal species<br />

collected from fishpond 5, confirmed as belonging to Achlya bisexualis species. The first<br />

fragment has 500 bp, the second one has about 200 bp. The third fragment is low visible.<br />

At the fungal species from Saprolegniaceae family, collected from Iernut fishery,<br />

Mureş county, with 5 fishponds, can be observed the same fragment lengths, in 1,2,3 and 5<br />

fishponds. The first fragment has 400 bp, the second one had 200 bp, and the third one 130-<br />

65

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