Rezumatul tezei de doctorat - USAMV Cluj-Napoca

DNA fragments, the first one between 350 and 400 bp, the second one around 200 bp, and
the third one around 150 bp. In the samples collected from 6 and 7 fishponds, there are
visualized 4 fragments: the first one between 450 bp and 500 bp, the second one around 300
bp, the third one around 200 bp, and the fourth one at 150 pb. Those samples of amplified
DNA are presenting a fragment length polymorphism, confirmed by the sequencing
analyses. The results indicated the presence of two aquatic fungal species, each one
belonging to another genera of Saprolegniaceae family. The DNA profiles, observed in
2,3,4,5,6,9 and 10 lanes, are belonging to Saprolegnia ferax species, and the DNA
polymorphic profiles, from 7 an 8 lanes, to Achlya bisexualis species.
At the fungal samples from Saprolegniaceae family, collected from Motiş fishery,
Sălaj county, in all the 7 analyzed fishponds, there is observed the same electrophoretic
profile of the PCR products, with 3 visible fragments. The first fragment has about 400 bp,
the second one 200 bp and the third one 150 bp. In all the analyzed samples, the
polymorphisms were the same, all the fragments indicating the presence of a single fungal
species, confirmed by sequencing as Saprolegnia ferax.
At the fungal species from Saprolegniaceae family, collected from Adrian fishery,
Satu Mare county, with 10 fishponds, in the 1,2,3,4,6,7,8 and 10 fishponds, there can be
visualized 3 migration lanes, the first one at about 380-400 bp, the second one at 200 bp and
the third one between 100 and 150 bp. After the sequence analyses performing, in the
respective fishponds was identified Saprolegnia ferax species. In the fishpond 5, there are
present 2 lanes, the first one at about 450 bp, and the second one at 200 bp. In the fishpond 9
can be observed precisely 4 fragments: the first one at about 450 bp, the second one at 250
bp, the third one at 200 bp and the fourth one between 100 and 150 bp. At the samples from
6 and 10 lanes, can be observed length polymorphisns, different from the rest of fungal DNA
samples, confirmed by sequencing as belonging to Achlya bisexualis species.
At the fungal species from Saprolegniaceae family, collected from łaga fishery, Cluj
county, there are present 4 restriction fragments, in all 5 analyzed fishponds. The first
fragment has 380-400 bp, the second one has 200 bp, the third one has 150 bp, and the fouth
one has around 100 bp. The electrophoretic profiles of DNA samples are similar as base
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