Rezumatul tezei de doctorat - USAMV Cluj-Napoca

1 2 3 4 5 6 7 8 9
700pb-
Fig.3. Electrophoretic profile of a DNA sample extracted from Saprolegnia and amplified
with ITS1 and ITS4, ITS4 and ITS5 primer pairs
1-Ladder Low Range of 700 bp (Fermentas); 2-DNA sample extracted with kit (ITS1-ITS4
primers); 3-DNA sample extracted with PBS solution (ITS1-ITS4 primers); 4-DNA sample
extracted with NE solution (ITS1-ITS4 primers); 5-DNA sample purified with PCR kit
(ITS1-ITS4 primers); 6-DNA sample purified with PCR kit (ITS4-ITS5 primers); 7-DNA
sample extracted with NE solution (ITS4-ITS5 primers); 8-DNA sample extracted with PBS
solution (ITS4-ITS5 primers); 9-DNA sample extracted with kit (ITS4-ITS5 primers)
II.6. THE DNA ENZYMATIC RESTRICTION RESULTS OF FUNGAL
SPECIES FROM SAPROLEGNIACEAE FAMILY USING PCR-RFLP METHOD
When we used AluI and HindIII restriction enzymes, the restriction didn’t perform.
The enzymes couldn’t digest the amplified fragment of 700 bp, because of the restriction
site. Only the third enzyme tested (RsaI), which restricted all the samples, was used in the
experiment to analyze the electrophoretic profile of the 69 samples, from the 10 locations.
Then were performed the electrophoretic profiles of the restriction analyses, with
RsaI (Fermentas) enzyme, of the DNA amplified with ITS1 and ITS4 primer pairs, at
Saprolegniaceae family fungal species.
The samples of fungal species from Saprolegniaceae family, from Ariniş fishery,
Maramureş county, with 9 fishponds, collected from the fishponds 1,2,3,4,5,8 and 9, had 3
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