Rezumatul tezei de doctorat - USAMV Cluj-Napoca

asexual one. We can precisely conclude that in specific laboratory conditions, all fungal
isolates had flagella on primary zoospores.
Regarding the development of sexual reproduction organs (antheridia and oogonia),
there are differences from on fishpond and location to another. This presence or absence of
the sexual reproduction can be attributed to the fact that the existent species are different,
and some of them do not realyze the sexual reproduction in vitro (Fregeneda Grandes, 2000;
Dieguez Uribeondo, 2007).
A statistical analysis regarding the development of sexual reproduction organs at the
analyzed fungal species from Saprolegniaceae family (69 samples), allows us to conclude
that 41 isolates (59,42%) have sexual reproduction organs, observed at the microscope. At
28 samples (40,58%), their presence was not visualized (table 3).
Hyphal
aspect
Table 3
Synthesis regarding the development of reproduction organs
at analyzed fungal species from Saprolegniaceae family
Sexual reproduction
Asexual
Present
Absent
reproduction
(Flagella presence)
No.of
samples
Percent
(%)
No.of
samples
Percent
(%)
No.of
samples
Percent
(%)
Unsegmented 41 59,42 28 40,58% 69 100%
II.4. RESULTS REGARDING SAPROLEGNIA’S DNA
EXTRACTION AND QUANTIFICATION
II.4.1. DNA extraction
Each sample of biologic material was divided, the two samples being tested by an
extraction method, to conclude which one of the two methods is more efficient for this type
of fungi, respecting the DNA quantity and purity.
The results of the 69 samples extracted by each method, are indicating that DNA
purities and quantities performed by Nanodrop ND 1000 spectrophotometer differ from one
fishpond and method to another.
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